Chromosome-level genome assembly of the Durum wheat cultivar Langdon
Abstract Durum wheat (Triticum turgidum ssp. durum, 2n = 4x = 28, BBAA) is the second most cultivated lineage of wheat after hexaploid common wheat. Langdon is a prominent durum wheat cultivar, highly valued for its crucial role in the development of synthetic hexaploid wheat and its importance in a...
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Nature Portfolio
2025-08-01
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| Series: | Scientific Data |
| Online Access: | https://doi.org/10.1038/s41597-025-05724-z |
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| author | Mengen Chen Junlong Li Yue Zhang Yanmeng Ding Wenjie Qin Mengyuan Wu Na Li Cuicui Jin Huili Wang Shuo Chen Dun Niu Xiao Sun Bingbing Zhang Shulin Chen Zhiyong Wang Cuilan Shi Kunpu Zhang Daowen Wang Shoufen Dai Zehong Yan Guangwei Li |
| author_facet | Mengen Chen Junlong Li Yue Zhang Yanmeng Ding Wenjie Qin Mengyuan Wu Na Li Cuicui Jin Huili Wang Shuo Chen Dun Niu Xiao Sun Bingbing Zhang Shulin Chen Zhiyong Wang Cuilan Shi Kunpu Zhang Daowen Wang Shoufen Dai Zehong Yan Guangwei Li |
| author_sort | Mengen Chen |
| collection | DOAJ |
| description | Abstract Durum wheat (Triticum turgidum ssp. durum, 2n = 4x = 28, BBAA) is the second most cultivated lineage of wheat after hexaploid common wheat. Langdon is a prominent durum wheat cultivar, highly valued for its crucial role in the development of synthetic hexaploid wheat and its importance in agricultural research. Here, we present a high-quality chromosome-level genome assembly of Langdon using a combination of HiFi sequencing and high-throughput chromosome conformation capture (Hi-C) techniques. The total genome assembly size was 10.47 Gbp, with a benchmarking universal single-copy orthologs (BUSCO) score of 98.80%, consensus nucleotide quality value (QV) score of 50.31, long terminal repeat assembly index (LAI) value of 19.39, a scaffold N50 reached to 751.30 Mbp and a contig N50 value of 41.18 Mbp. Repetitive elements constituted 88.22% of the assembly, and 68,342 high confidence (HC) genes were annotated. The high-quality chromosome-level Langdon assembly can serve as a valuable reference for durum wheat, aiding gene research and comparative genomics, and leveraging synthetic hexaploid wheat’s genetic resources to advance global wheat improvement. |
| format | Article |
| id | doaj-art-47bf9f0cd6344f5e8a08caa4045667ec |
| institution | Kabale University |
| issn | 2052-4463 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | Nature Portfolio |
| record_format | Article |
| series | Scientific Data |
| spelling | doaj-art-47bf9f0cd6344f5e8a08caa4045667ec2025-08-20T03:45:45ZengNature PortfolioScientific Data2052-44632025-08-0112111110.1038/s41597-025-05724-zChromosome-level genome assembly of the Durum wheat cultivar LangdonMengen Chen0Junlong Li1Yue Zhang2Yanmeng Ding3Wenjie Qin4Mengyuan Wu5Na Li6Cuicui Jin7Huili Wang8Shuo Chen9Dun Niu10Xiao Sun11Bingbing Zhang12Shulin Chen13Zhiyong Wang14Cuilan Shi15Kunpu Zhang16Daowen Wang17Shoufen Dai18Zehong Yan19Guangwei Li20State Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Henan Center for Crop genomics and Rice Engineering, College of Agronomy, Longzi Lake Campus, Henan Agricultural UniversityState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Henan Center for Crop genomics and Rice Engineering, College of Agronomy, Longzi Lake Campus, Henan Agricultural UniversityState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Henan Center for Crop genomics and Rice Engineering, College of Agronomy, Longzi Lake Campus, Henan Agricultural UniversityState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Henan Center for Crop genomics and Rice Engineering, College of Agronomy, Longzi Lake Campus, Henan Agricultural UniversityState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Henan Center for Crop genomics and Rice Engineering, College of Agronomy, Longzi Lake Campus, Henan Agricultural UniversityState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Henan Center for Crop genomics and Rice Engineering, College of Agronomy, Longzi Lake Campus, Henan Agricultural UniversityState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Henan Center for Crop genomics and Rice Engineering, College of Agronomy, Longzi Lake Campus, Henan Agricultural UniversityState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Henan Center for Crop genomics and Rice Engineering, College of Agronomy, Longzi Lake Campus, Henan Agricultural UniversityState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Henan Center for Crop genomics and Rice Engineering, College of Agronomy, Longzi Lake Campus, Henan Agricultural UniversityState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Henan Center for Crop genomics and Rice Engineering, College of Agronomy, Longzi Lake Campus, Henan Agricultural UniversityState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Henan Center for Crop genomics and Rice Engineering, College of Agronomy, Longzi Lake Campus, Henan Agricultural UniversityState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Henan Center for Crop genomics and Rice Engineering, College of Agronomy, Longzi Lake Campus, Henan Agricultural UniversityState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Henan Center for Crop genomics and Rice Engineering, College of Agronomy, Longzi Lake Campus, Henan Agricultural UniversityState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Henan Center for Crop genomics and Rice Engineering, College of Agronomy, Longzi Lake Campus, Henan Agricultural UniversityState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Henan Center for Crop genomics and Rice Engineering, College of Agronomy, Longzi Lake Campus, Henan Agricultural UniversityState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Henan Center for Crop genomics and Rice Engineering, College of Agronomy, Longzi Lake Campus, Henan Agricultural UniversityState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Henan Center for Crop genomics and Rice Engineering, College of Agronomy, Longzi Lake Campus, Henan Agricultural UniversityState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Henan Center for Crop genomics and Rice Engineering, College of Agronomy, Longzi Lake Campus, Henan Agricultural UniversityTriticeae Research Institute, Sichuan Agricultural UniversityTriticeae Research Institute, Sichuan Agricultural UniversityState Key Laboratory of High-Efficiency Production of Wheat-Maize Double Cropping, Henan Center for Crop genomics and Rice Engineering, College of Agronomy, Longzi Lake Campus, Henan Agricultural UniversityAbstract Durum wheat (Triticum turgidum ssp. durum, 2n = 4x = 28, BBAA) is the second most cultivated lineage of wheat after hexaploid common wheat. Langdon is a prominent durum wheat cultivar, highly valued for its crucial role in the development of synthetic hexaploid wheat and its importance in agricultural research. Here, we present a high-quality chromosome-level genome assembly of Langdon using a combination of HiFi sequencing and high-throughput chromosome conformation capture (Hi-C) techniques. The total genome assembly size was 10.47 Gbp, with a benchmarking universal single-copy orthologs (BUSCO) score of 98.80%, consensus nucleotide quality value (QV) score of 50.31, long terminal repeat assembly index (LAI) value of 19.39, a scaffold N50 reached to 751.30 Mbp and a contig N50 value of 41.18 Mbp. Repetitive elements constituted 88.22% of the assembly, and 68,342 high confidence (HC) genes were annotated. The high-quality chromosome-level Langdon assembly can serve as a valuable reference for durum wheat, aiding gene research and comparative genomics, and leveraging synthetic hexaploid wheat’s genetic resources to advance global wheat improvement.https://doi.org/10.1038/s41597-025-05724-z |
| spellingShingle | Mengen Chen Junlong Li Yue Zhang Yanmeng Ding Wenjie Qin Mengyuan Wu Na Li Cuicui Jin Huili Wang Shuo Chen Dun Niu Xiao Sun Bingbing Zhang Shulin Chen Zhiyong Wang Cuilan Shi Kunpu Zhang Daowen Wang Shoufen Dai Zehong Yan Guangwei Li Chromosome-level genome assembly of the Durum wheat cultivar Langdon Scientific Data |
| title | Chromosome-level genome assembly of the Durum wheat cultivar Langdon |
| title_full | Chromosome-level genome assembly of the Durum wheat cultivar Langdon |
| title_fullStr | Chromosome-level genome assembly of the Durum wheat cultivar Langdon |
| title_full_unstemmed | Chromosome-level genome assembly of the Durum wheat cultivar Langdon |
| title_short | Chromosome-level genome assembly of the Durum wheat cultivar Langdon |
| title_sort | chromosome level genome assembly of the durum wheat cultivar langdon |
| url | https://doi.org/10.1038/s41597-025-05724-z |
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