Chromosome-level genome assembly of the Durum wheat cultivar Langdon

Abstract Durum wheat (Triticum turgidum ssp. durum, 2n = 4x = 28, BBAA) is the second most cultivated lineage of wheat after hexaploid common wheat. Langdon is a prominent durum wheat cultivar, highly valued for its crucial role in the development of synthetic hexaploid wheat and its importance in a...

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Main Authors: Mengen Chen, Junlong Li, Yue Zhang, Yanmeng Ding, Wenjie Qin, Mengyuan Wu, Na Li, Cuicui Jin, Huili Wang, Shuo Chen, Dun Niu, Xiao Sun, Bingbing Zhang, Shulin Chen, Zhiyong Wang, Cuilan Shi, Kunpu Zhang, Daowen Wang, Shoufen Dai, Zehong Yan, Guangwei Li
Format: Article
Language:English
Published: Nature Portfolio 2025-08-01
Series:Scientific Data
Online Access:https://doi.org/10.1038/s41597-025-05724-z
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Summary:Abstract Durum wheat (Triticum turgidum ssp. durum, 2n = 4x = 28, BBAA) is the second most cultivated lineage of wheat after hexaploid common wheat. Langdon is a prominent durum wheat cultivar, highly valued for its crucial role in the development of synthetic hexaploid wheat and its importance in agricultural research. Here, we present a high-quality chromosome-level genome assembly of Langdon using a combination of HiFi sequencing and high-throughput chromosome conformation capture (Hi-C) techniques. The total genome assembly size was 10.47 Gbp, with a benchmarking universal single-copy orthologs (BUSCO) score of 98.80%, consensus nucleotide quality value (QV) score of 50.31, long terminal repeat assembly index (LAI) value of 19.39, a scaffold N50 reached to 751.30 Mbp and a contig N50 value of 41.18 Mbp. Repetitive elements constituted 88.22% of the assembly, and 68,342 high confidence (HC) genes were annotated. The high-quality chromosome-level Langdon assembly can serve as a valuable reference for durum wheat, aiding gene research and comparative genomics, and leveraging synthetic hexaploid wheat’s genetic resources to advance global wheat improvement.
ISSN:2052-4463