Fabrication of Gliadin–Carboxymethyl Chitosan Composite Nanoparticles to Improve the Stability and Antioxidant Activity of Curcumin

The antisolvent precipitation method was employed to synthesize curcumin-loaded gliadin–carboxymethyl chitosan (CMCS) composite nanoparticles (GCC NPs). When the gliadin/CMCS weight ratio was 2:1, the GCC NPs with an ideal negative charge (−27.57 ± 1.07 mV) and the minimum particle size (184.13 ± 5....

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Main Authors: Xinyue Zhang, Mengdie Mo, Haiqi Yu, Hua Yang, Xu Liu, Yaping Xu, Xiaohui Zheng, Jie Wei, Fei Yu, Xiaodong Chen
Format: Article
Language:English
Published: MDPI AG 2025-05-01
Series:Molecules
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Online Access:https://www.mdpi.com/1420-3049/30/11/2414
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author Xinyue Zhang
Mengdie Mo
Haiqi Yu
Hua Yang
Xu Liu
Yaping Xu
Xiaohui Zheng
Jie Wei
Fei Yu
Xiaodong Chen
author_facet Xinyue Zhang
Mengdie Mo
Haiqi Yu
Hua Yang
Xu Liu
Yaping Xu
Xiaohui Zheng
Jie Wei
Fei Yu
Xiaodong Chen
author_sort Xinyue Zhang
collection DOAJ
description The antisolvent precipitation method was employed to synthesize curcumin-loaded gliadin–carboxymethyl chitosan (CMCS) composite nanoparticles (GCC NPs). When the gliadin/CMCS weight ratio was 2:1, the GCC NPs with an ideal negative charge (−27.57 ± 1.07 mV) and the minimum particle size (184.13 ± 5.49 nm) were obtained. With the addition of CMCS, the encapsulation efficiency (EE) of Cur was markedly improved from 77.46 ± 1.54% to 93.88 ± 1.31%. Under various pH values and salt concentrations, the GCC NPs displayed excellent colloidal stability. Specifically, after encapsulation within the GCC NPs, the antioxidant activity of Cur was markedly improved. In ABTS and DPPH assays, the SC<sub data-eusoft-scrollable-element="1">50</sub> values of the GCC NPs were 4.98 ± 0.07 µg/mL and 9.86 ± 0.29 µg/mL, respectively. In summary, the GCC NPs would be an effective platform for the delivery of Cur in food and pharmaceutical preparations.
format Article
id doaj-art-47b0da751c174d44b7a534a63690cb10
institution OA Journals
issn 1420-3049
language English
publishDate 2025-05-01
publisher MDPI AG
record_format Article
series Molecules
spelling doaj-art-47b0da751c174d44b7a534a63690cb102025-08-20T02:23:08ZengMDPI AGMolecules1420-30492025-05-013011241410.3390/molecules30112414Fabrication of Gliadin–Carboxymethyl Chitosan Composite Nanoparticles to Improve the Stability and Antioxidant Activity of CurcuminXinyue Zhang0Mengdie Mo1Haiqi Yu2Hua Yang3Xu Liu4Yaping Xu5Xiaohui Zheng6Jie Wei7Fei Yu8Xiaodong Chen9Medical College, Guangxi University, Nanning 530004, ChinaMedical College, Guangxi University, Nanning 530004, ChinaMedical College, Guangxi University, Nanning 530004, ChinaMedical College, Guangxi University, Nanning 530004, ChinaMedical College, Guangxi University, Nanning 530004, ChinaKey Laboratory of Functional and Clinical Translational Medicine, Xiamen Medical College, Fujian Provincial University, Xiamen 361023, ChinaKey Laboratory of Functional and Clinical Translational Medicine, Xiamen Medical College, Fujian Provincial University, Xiamen 361023, ChinaCollege of Agriculture, Guangxi University, Nanning 530004, ChinaMedical College, Guangxi University, Nanning 530004, ChinaSuzhou Key Lab of Green Chemical Engineering, School of Chemical and Environmental Engineering, College of Chemistry, Chemical Engineering and Materials Science, Soochow University, Suzhou 215123, ChinaThe antisolvent precipitation method was employed to synthesize curcumin-loaded gliadin–carboxymethyl chitosan (CMCS) composite nanoparticles (GCC NPs). When the gliadin/CMCS weight ratio was 2:1, the GCC NPs with an ideal negative charge (−27.57 ± 1.07 mV) and the minimum particle size (184.13 ± 5.49 nm) were obtained. With the addition of CMCS, the encapsulation efficiency (EE) of Cur was markedly improved from 77.46 ± 1.54% to 93.88 ± 1.31%. Under various pH values and salt concentrations, the GCC NPs displayed excellent colloidal stability. Specifically, after encapsulation within the GCC NPs, the antioxidant activity of Cur was markedly improved. In ABTS and DPPH assays, the SC<sub data-eusoft-scrollable-element="1">50</sub> values of the GCC NPs were 4.98 ± 0.07 µg/mL and 9.86 ± 0.29 µg/mL, respectively. In summary, the GCC NPs would be an effective platform for the delivery of Cur in food and pharmaceutical preparations.https://www.mdpi.com/1420-3049/30/11/2414gliadincurcumincarboxymethyl chitosannanoparticlesantioxidant activityfood
spellingShingle Xinyue Zhang
Mengdie Mo
Haiqi Yu
Hua Yang
Xu Liu
Yaping Xu
Xiaohui Zheng
Jie Wei
Fei Yu
Xiaodong Chen
Fabrication of Gliadin–Carboxymethyl Chitosan Composite Nanoparticles to Improve the Stability and Antioxidant Activity of Curcumin
Molecules
gliadin
curcumin
carboxymethyl chitosan
nanoparticles
antioxidant activity
food
title Fabrication of Gliadin–Carboxymethyl Chitosan Composite Nanoparticles to Improve the Stability and Antioxidant Activity of Curcumin
title_full Fabrication of Gliadin–Carboxymethyl Chitosan Composite Nanoparticles to Improve the Stability and Antioxidant Activity of Curcumin
title_fullStr Fabrication of Gliadin–Carboxymethyl Chitosan Composite Nanoparticles to Improve the Stability and Antioxidant Activity of Curcumin
title_full_unstemmed Fabrication of Gliadin–Carboxymethyl Chitosan Composite Nanoparticles to Improve the Stability and Antioxidant Activity of Curcumin
title_short Fabrication of Gliadin–Carboxymethyl Chitosan Composite Nanoparticles to Improve the Stability and Antioxidant Activity of Curcumin
title_sort fabrication of gliadin carboxymethyl chitosan composite nanoparticles to improve the stability and antioxidant activity of curcumin
topic gliadin
curcumin
carboxymethyl chitosan
nanoparticles
antioxidant activity
food
url https://www.mdpi.com/1420-3049/30/11/2414
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