Comparison of the Effects of Retraining After Lower Limb Suspension on the Expression of MURF1 and TRAF6 Genes in Slow- and Fast-Twitch Muscles of Male Rats
Background and purpose: Exercise interventions are recognized as effective strategies for mitigating muscle atrophy. The present study aimed to investigate the effects of retraining, administered both before and after a period of lower limb suspension, on the expression of MURF1 and TRAF6 genes in s...
Saved in:
| Main Authors: | , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Mazandaran University of Medical Sciences
2025-07-01
|
| Series: | Journal of Mazandaran University of Medical Sciences |
| Subjects: | |
| Online Access: | http://jmums.mazums.ac.ir/article-1-21821-en.pdf |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849470634669637632 |
|---|---|
| author | Sudabeh Hassanshahi Abdolreza Kazemi Ayat Kaeidi |
| author_facet | Sudabeh Hassanshahi Abdolreza Kazemi Ayat Kaeidi |
| author_sort | Sudabeh Hassanshahi |
| collection | DOAJ |
| description | Background and purpose: Exercise interventions are recognized as effective strategies for mitigating muscle atrophy. The present study aimed to investigate the effects of retraining, administered both before and after a period of lower limb suspension, on the expression of MURF1 and TRAF6 genes in slow- and fast-twitch muscles of male rats.
Materials and methods: In this experimental study, 32 rats were subjected to an exhausting treadmill test 48 hours after the last familiarization session which lasted one week, running at a speed of 10 to 18 meters per minute for ten minutes. The animals were divided into four groups of eight animals based on their maximum speed. The duration of the training protocol was five weeks, and the duration of the lower limb suspension was two weeks. Forty-eight hours after the last training session, the soleus muscle and the abductor digitorum longus muscle were extracted, and the expression levels of MURF1 and TRAF6 genes were measured using real-time PCR.
Results: MURF1 and TRAF6 gene expression was significantly reduced by high-intensity interval training (HIIT) in slow- and fast-twitch muscles of male rats compared to the control group (P< 0.05). However, in the slow-twitch muscle (soleus), there was no significant difference in gene expression between the training groups (P> 0.05). In contrast, in the fast-twitch muscle (extensor digitorum longus), there was a significant difference in MURF1 gene expression in the retraining group compared to other groups (P< 0.05). TRAF6 gene expression in the training groups was also significantly different only between the retraining and non-training groups (P< 0.05)
Conclusion: HIIT training before and after lower limb suspension appears to reduce atrophy in slow- and fast-twitch muscles of male rats; however, fast-twitch muscles in the retraining group demonstrated faster recovery than slow-twitch muscles. |
| format | Article |
| id | doaj-art-47a5cec192af4e489e38277a7b5c311d |
| institution | Kabale University |
| issn | 1735-9260 1735-9279 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Mazandaran University of Medical Sciences |
| record_format | Article |
| series | Journal of Mazandaran University of Medical Sciences |
| spelling | doaj-art-47a5cec192af4e489e38277a7b5c311d2025-08-20T03:25:07ZengMazandaran University of Medical SciencesJournal of Mazandaran University of Medical Sciences1735-92601735-92792025-07-01352462235Comparison of the Effects of Retraining After Lower Limb Suspension on the Expression of MURF1 and TRAF6 Genes in Slow- and Fast-Twitch Muscles of Male RatsSudabeh Hassanshahi0Abdolreza Kazemi1Ayat Kaeidi2 MSc in Exercise Physiology, Department of Sport Sciences, Faculty of Literature and Humanities, Vali-E-Asr University of Rafsanjan, Rafsanjan, Iran Associate Professor, Department of Sport Sciences, Faculty of Literature and Humanities, Vali-E-Asr University of Rafsanjan, Rafsanjan, Iran Associate Professor, Department of Physiology and Pharmacology, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran Background and purpose: Exercise interventions are recognized as effective strategies for mitigating muscle atrophy. The present study aimed to investigate the effects of retraining, administered both before and after a period of lower limb suspension, on the expression of MURF1 and TRAF6 genes in slow- and fast-twitch muscles of male rats. Materials and methods: In this experimental study, 32 rats were subjected to an exhausting treadmill test 48 hours after the last familiarization session which lasted one week, running at a speed of 10 to 18 meters per minute for ten minutes. The animals were divided into four groups of eight animals based on their maximum speed. The duration of the training protocol was five weeks, and the duration of the lower limb suspension was two weeks. Forty-eight hours after the last training session, the soleus muscle and the abductor digitorum longus muscle were extracted, and the expression levels of MURF1 and TRAF6 genes were measured using real-time PCR. Results: MURF1 and TRAF6 gene expression was significantly reduced by high-intensity interval training (HIIT) in slow- and fast-twitch muscles of male rats compared to the control group (P< 0.05). However, in the slow-twitch muscle (soleus), there was no significant difference in gene expression between the training groups (P> 0.05). In contrast, in the fast-twitch muscle (extensor digitorum longus), there was a significant difference in MURF1 gene expression in the retraining group compared to other groups (P< 0.05). TRAF6 gene expression in the training groups was also significantly different only between the retraining and non-training groups (P< 0.05) Conclusion: HIIT training before and after lower limb suspension appears to reduce atrophy in slow- and fast-twitch muscles of male rats; however, fast-twitch muscles in the retraining group demonstrated faster recovery than slow-twitch muscles.http://jmums.mazums.ac.ir/article-1-21821-en.pdfhiit trainingretrainingfast and slow twitch muscleslower limb suspensionmuscle atrophy |
| spellingShingle | Sudabeh Hassanshahi Abdolreza Kazemi Ayat Kaeidi Comparison of the Effects of Retraining After Lower Limb Suspension on the Expression of MURF1 and TRAF6 Genes in Slow- and Fast-Twitch Muscles of Male Rats Journal of Mazandaran University of Medical Sciences hiit training retraining fast and slow twitch muscles lower limb suspension muscle atrophy |
| title | Comparison of the Effects of Retraining After Lower Limb Suspension on the Expression of MURF1 and TRAF6 Genes in Slow- and Fast-Twitch Muscles of Male Rats |
| title_full | Comparison of the Effects of Retraining After Lower Limb Suspension on the Expression of MURF1 and TRAF6 Genes in Slow- and Fast-Twitch Muscles of Male Rats |
| title_fullStr | Comparison of the Effects of Retraining After Lower Limb Suspension on the Expression of MURF1 and TRAF6 Genes in Slow- and Fast-Twitch Muscles of Male Rats |
| title_full_unstemmed | Comparison of the Effects of Retraining After Lower Limb Suspension on the Expression of MURF1 and TRAF6 Genes in Slow- and Fast-Twitch Muscles of Male Rats |
| title_short | Comparison of the Effects of Retraining After Lower Limb Suspension on the Expression of MURF1 and TRAF6 Genes in Slow- and Fast-Twitch Muscles of Male Rats |
| title_sort | comparison of the effects of retraining after lower limb suspension on the expression of murf1 and traf6 genes in slow and fast twitch muscles of male rats |
| topic | hiit training retraining fast and slow twitch muscles lower limb suspension muscle atrophy |
| url | http://jmums.mazums.ac.ir/article-1-21821-en.pdf |
| work_keys_str_mv | AT sudabehhassanshahi comparisonoftheeffectsofretrainingafterlowerlimbsuspensionontheexpressionofmurf1andtraf6genesinslowandfasttwitchmusclesofmalerats AT abdolrezakazemi comparisonoftheeffectsofretrainingafterlowerlimbsuspensionontheexpressionofmurf1andtraf6genesinslowandfasttwitchmusclesofmalerats AT ayatkaeidi comparisonoftheeffectsofretrainingafterlowerlimbsuspensionontheexpressionofmurf1andtraf6genesinslowandfasttwitchmusclesofmalerats |