Route of Application and Dose Evaluation of Dental Pulp Stem Cells for the Treatment of Sialadenitis Caused by Sjögren’s Syndrome: A Preclinical Study
<b>Background:</b> Sjögren’s syndrome (SS) is an autoimmune disorder characterized by sicca syndrome and/or systemic manifestations. In this study, non-obese diabetic (<i>NOD</i>) mice were used as an animal model for studying SS, to evaluate the optimal administration route...
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2025-04-01
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| author | Zhihao Du Lifang Feng Yu Zhang Xin Peng Shan Zhang Rui Zhao Jia Lei Xiaotong Li Guangyan Yu Chong Ding |
| author_facet | Zhihao Du Lifang Feng Yu Zhang Xin Peng Shan Zhang Rui Zhao Jia Lei Xiaotong Li Guangyan Yu Chong Ding |
| author_sort | Zhihao Du |
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| description | <b>Background:</b> Sjögren’s syndrome (SS) is an autoimmune disorder characterized by sicca syndrome and/or systemic manifestations. In this study, non-obese diabetic (<i>NOD</i>) mice were used as an animal model for studying SS, to evaluate the optimal administration route and dose range of dental pulp stem cells (DPSCs) in the treatment of sialadenitis caused by SS. <b>Methods:</b> Different doses of DPSCs were transplanted into the submandibular glands (SMGs) of 14-week-old <i>NOD</i> mice through two different methods: injection or retrograde perfusion through the catheter orifice into the SMG. At 21 weeks of age, the saliva flow rate (SFR), ectopic lymphocytes, and CD4<sup>+</sup> T-cell infiltration were measured. Tumor necrosis factor-alpha (TNF-α) and interferon-gamma (IFN-γ) in the glandular tissues were also quantitatively detected. <b>Results:</b> Compared with untreated and PBS-injected controls, different-dose groups of the two administration methods showed an increased saliva flow rate of <i>NOD</i> mice to varying degrees, reduced infiltration of lymphocytes and CD4<sup>+</sup> T cells in the SMG, and decreased IFN-γ/TNF-α levels. Finally, we compared these two administration routes and found that the perfusion of 2 × 10<sup>5</sup> DPSCs presents good therapeutic effects. <b>Conclusions:</b> DPSC perfusion through the catheter orifice is a simple and effective treatment method, which is worthy of further investigation through clinical trials. |
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| institution | Kabale University |
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| language | English |
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| series | Biomedicines |
| spelling | doaj-art-47a0a80dc8794e38a49805eaa00a759d2025-08-20T03:47:53ZengMDPI AGBiomedicines2227-90592025-04-01135106810.3390/biomedicines13051068Route of Application and Dose Evaluation of Dental Pulp Stem Cells for the Treatment of Sialadenitis Caused by Sjögren’s Syndrome: A Preclinical StudyZhihao Du0Lifang Feng1Yu Zhang2Xin Peng3Shan Zhang4Rui Zhao5Jia Lei6Xiaotong Li7Guangyan Yu8Chong Ding9Department of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, No. 22, Zhongguancun South Avenue, Haidian District, Beijing 100081, ChinaDepartment of Prosthodontics, North China University of Science and Technology, Stomatology, Tangshan Bay Ecological Zone No. 21 Bohai Avenue, Tangshan 063210, ChinaDepartment of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, No. 22, Zhongguancun South Avenue, Haidian District, Beijing 100081, ChinaDepartment of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, No. 22, Zhongguancun South Avenue, Haidian District, Beijing 100081, ChinaCenter Laboratory, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Beijing Key Laboratory of Digital Stomatology & NHC Key Laboratory of Digital Stomatology & NMPA Key Laboratory for Dental Materials, No. 22, Zhongguancun South Avenue, Haidian District, Beijing 100081, ChinaCenter Laboratory, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Beijing Key Laboratory of Digital Stomatology & NHC Key Laboratory of Digital Stomatology & NMPA Key Laboratory for Dental Materials, No. 22, Zhongguancun South Avenue, Haidian District, Beijing 100081, ChinaCenter Laboratory, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Beijing Key Laboratory of Digital Stomatology & NHC Key Laboratory of Digital Stomatology & NMPA Key Laboratory for Dental Materials, No. 22, Zhongguancun South Avenue, Haidian District, Beijing 100081, ChinaCenter Laboratory, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Beijing Key Laboratory of Digital Stomatology & NHC Key Laboratory of Digital Stomatology & NMPA Key Laboratory for Dental Materials, No. 22, Zhongguancun South Avenue, Haidian District, Beijing 100081, ChinaDepartment of Oral and Maxillofacial Surgery, Peking University School and Hospital of Stomatology, No. 22, Zhongguancun South Avenue, Haidian District, Beijing 100081, ChinaCenter Laboratory, Peking University School and Hospital of Stomatology & National Center for Stomatology & National Clinical Research Center for Oral Diseases & National Engineering Research Center of Oral Biomaterials and Digital Medical Devices & Beijing Key Laboratory of Digital Stomatology & NHC Key Laboratory of Digital Stomatology & NMPA Key Laboratory for Dental Materials, No. 22, Zhongguancun South Avenue, Haidian District, Beijing 100081, China<b>Background:</b> Sjögren’s syndrome (SS) is an autoimmune disorder characterized by sicca syndrome and/or systemic manifestations. In this study, non-obese diabetic (<i>NOD</i>) mice were used as an animal model for studying SS, to evaluate the optimal administration route and dose range of dental pulp stem cells (DPSCs) in the treatment of sialadenitis caused by SS. <b>Methods:</b> Different doses of DPSCs were transplanted into the submandibular glands (SMGs) of 14-week-old <i>NOD</i> mice through two different methods: injection or retrograde perfusion through the catheter orifice into the SMG. At 21 weeks of age, the saliva flow rate (SFR), ectopic lymphocytes, and CD4<sup>+</sup> T-cell infiltration were measured. Tumor necrosis factor-alpha (TNF-α) and interferon-gamma (IFN-γ) in the glandular tissues were also quantitatively detected. <b>Results:</b> Compared with untreated and PBS-injected controls, different-dose groups of the two administration methods showed an increased saliva flow rate of <i>NOD</i> mice to varying degrees, reduced infiltration of lymphocytes and CD4<sup>+</sup> T cells in the SMG, and decreased IFN-γ/TNF-α levels. Finally, we compared these two administration routes and found that the perfusion of 2 × 10<sup>5</sup> DPSCs presents good therapeutic effects. <b>Conclusions:</b> DPSC perfusion through the catheter orifice is a simple and effective treatment method, which is worthy of further investigation through clinical trials.https://www.mdpi.com/2227-9059/13/5/1068Sjögren’s syndromestem cell therapydental pulp stem cellstreatment strategiesprecision medicine |
| spellingShingle | Zhihao Du Lifang Feng Yu Zhang Xin Peng Shan Zhang Rui Zhao Jia Lei Xiaotong Li Guangyan Yu Chong Ding Route of Application and Dose Evaluation of Dental Pulp Stem Cells for the Treatment of Sialadenitis Caused by Sjögren’s Syndrome: A Preclinical Study Biomedicines Sjögren’s syndrome stem cell therapy dental pulp stem cells treatment strategies precision medicine |
| title | Route of Application and Dose Evaluation of Dental Pulp Stem Cells for the Treatment of Sialadenitis Caused by Sjögren’s Syndrome: A Preclinical Study |
| title_full | Route of Application and Dose Evaluation of Dental Pulp Stem Cells for the Treatment of Sialadenitis Caused by Sjögren’s Syndrome: A Preclinical Study |
| title_fullStr | Route of Application and Dose Evaluation of Dental Pulp Stem Cells for the Treatment of Sialadenitis Caused by Sjögren’s Syndrome: A Preclinical Study |
| title_full_unstemmed | Route of Application and Dose Evaluation of Dental Pulp Stem Cells for the Treatment of Sialadenitis Caused by Sjögren’s Syndrome: A Preclinical Study |
| title_short | Route of Application and Dose Evaluation of Dental Pulp Stem Cells for the Treatment of Sialadenitis Caused by Sjögren’s Syndrome: A Preclinical Study |
| title_sort | route of application and dose evaluation of dental pulp stem cells for the treatment of sialadenitis caused by sjogren s syndrome a preclinical study |
| topic | Sjögren’s syndrome stem cell therapy dental pulp stem cells treatment strategies precision medicine |
| url | https://www.mdpi.com/2227-9059/13/5/1068 |
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