Spatiotemporal properties of cortical excitatory and inhibitory neuron activation by sustained and bursting electrical microstimulation
Summary: Intracortical microstimulation (ICMS) of sensory brain regions can create artificial sensations, yet these percepts fade with continued stimulation, suggesting dynamic changes in underlying neural activity. Using two-photon imaging in transgenic mice, this study examines how prolonged ICMS...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-06-01
|
| Series: | iScience |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S258900422500968X |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850131099568570368 |
|---|---|
| author | Christopher L. Hughes Kevin C. Stieger Keying Chen Alberto L. Vazquez Takashi D.Y. Kozai |
| author_facet | Christopher L. Hughes Kevin C. Stieger Keying Chen Alberto L. Vazquez Takashi D.Y. Kozai |
| author_sort | Christopher L. Hughes |
| collection | DOAJ |
| description | Summary: Intracortical microstimulation (ICMS) of sensory brain regions can create artificial sensations, yet these percepts fade with continued stimulation, suggesting dynamic changes in underlying neural activity. Using two-photon imaging in transgenic mice, this study examines how prolonged ICMS (30 s) shapes activity in excitatory and inhibitory neurons within the visual cortex. Inhibitory neuron activity was more likely to increase throughout the stimulation period, while excitatory neuron activity was more likely to decrease and be suppressed post-stimulation. Stimulation patterns differentially shaped neuronal engagement: theta-burst stimulation most effectively activated inhibitory neurons, whereas 10-Hz burst most effectively activated excitatory neurons. ICMS evoked more diverse responses in neurons farther from the electrode, reflecting complex synaptic interactions involving inhibition and disinhibition. These results show that ICMS affects excitatory and inhibitory neurons differently over extended durations. Modulation of stimulation patterns may allow for preferential engagement of specific neuron types and shaping of cortical activity. |
| format | Article |
| id | doaj-art-479b076bb4f144c18d7e98d43f77984c |
| institution | OA Journals |
| issn | 2589-0042 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | iScience |
| spelling | doaj-art-479b076bb4f144c18d7e98d43f77984c2025-08-20T02:32:31ZengElsevieriScience2589-00422025-06-0128611270710.1016/j.isci.2025.112707Spatiotemporal properties of cortical excitatory and inhibitory neuron activation by sustained and bursting electrical microstimulationChristopher L. Hughes0Kevin C. Stieger1Keying Chen2Alberto L. Vazquez3Takashi D.Y. Kozai4Department of Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA; Center for the Neural Basis of Cognition, Pittsburgh, PA, USADepartment of Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA; Center for the Neural Basis of Cognition, Pittsburgh, PA, USADepartment of Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA; Center for the Neural Basis of Cognition, Pittsburgh, PA, USADepartment of Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA; Center for the Neural Basis of Cognition, Pittsburgh, PA, USA; Center for Neuroscience, University of Pittsburgh, Pittsburgh, PA, USA; Department of Radiology, University of Pittsburgh, Pittsburgh, PA, USADepartment of Bioengineering, University of Pittsburgh, Pittsburgh, PA, USA; Center for the Neural Basis of Cognition, Pittsburgh, PA, USA; Center for Neuroscience, University of Pittsburgh, Pittsburgh, PA, USA; McGowan Institute for Regenerative Medicine, University of Pittsburgh, Pittsburgh, PA, USA; NeuroTech Center, University of Pittsburgh Brain Institute, Pittsburgh, PA, USA; Corresponding authorSummary: Intracortical microstimulation (ICMS) of sensory brain regions can create artificial sensations, yet these percepts fade with continued stimulation, suggesting dynamic changes in underlying neural activity. Using two-photon imaging in transgenic mice, this study examines how prolonged ICMS (30 s) shapes activity in excitatory and inhibitory neurons within the visual cortex. Inhibitory neuron activity was more likely to increase throughout the stimulation period, while excitatory neuron activity was more likely to decrease and be suppressed post-stimulation. Stimulation patterns differentially shaped neuronal engagement: theta-burst stimulation most effectively activated inhibitory neurons, whereas 10-Hz burst most effectively activated excitatory neurons. ICMS evoked more diverse responses in neurons farther from the electrode, reflecting complex synaptic interactions involving inhibition and disinhibition. These results show that ICMS affects excitatory and inhibitory neurons differently over extended durations. Modulation of stimulation patterns may allow for preferential engagement of specific neuron types and shaping of cortical activity.http://www.sciencedirect.com/science/article/pii/S258900422500968XNeuroscienceSensory neuroscience |
| spellingShingle | Christopher L. Hughes Kevin C. Stieger Keying Chen Alberto L. Vazquez Takashi D.Y. Kozai Spatiotemporal properties of cortical excitatory and inhibitory neuron activation by sustained and bursting electrical microstimulation iScience Neuroscience Sensory neuroscience |
| title | Spatiotemporal properties of cortical excitatory and inhibitory neuron activation by sustained and bursting electrical microstimulation |
| title_full | Spatiotemporal properties of cortical excitatory and inhibitory neuron activation by sustained and bursting electrical microstimulation |
| title_fullStr | Spatiotemporal properties of cortical excitatory and inhibitory neuron activation by sustained and bursting electrical microstimulation |
| title_full_unstemmed | Spatiotemporal properties of cortical excitatory and inhibitory neuron activation by sustained and bursting electrical microstimulation |
| title_short | Spatiotemporal properties of cortical excitatory and inhibitory neuron activation by sustained and bursting electrical microstimulation |
| title_sort | spatiotemporal properties of cortical excitatory and inhibitory neuron activation by sustained and bursting electrical microstimulation |
| topic | Neuroscience Sensory neuroscience |
| url | http://www.sciencedirect.com/science/article/pii/S258900422500968X |
| work_keys_str_mv | AT christopherlhughes spatiotemporalpropertiesofcorticalexcitatoryandinhibitoryneuronactivationbysustainedandburstingelectricalmicrostimulation AT kevincstieger spatiotemporalpropertiesofcorticalexcitatoryandinhibitoryneuronactivationbysustainedandburstingelectricalmicrostimulation AT keyingchen spatiotemporalpropertiesofcorticalexcitatoryandinhibitoryneuronactivationbysustainedandburstingelectricalmicrostimulation AT albertolvazquez spatiotemporalpropertiesofcorticalexcitatoryandinhibitoryneuronactivationbysustainedandburstingelectricalmicrostimulation AT takashidykozai spatiotemporalpropertiesofcorticalexcitatoryandinhibitoryneuronactivationbysustainedandburstingelectricalmicrostimulation |