<i>Paenibacillus hubeiensis</i> sp. nov.: A Novel Selenium-Resistant Bacterium Isolated from the Rhizosphere of <i>Galinsoga parviflora</i> in a Selenium-Rich Region of Enshi, Hubei Province
ES5-4<sup>T</sup>, a Gram-positive, motile, aerobic, and rod-shaped strain, was isolated from the rhizosphere of <i>Galinsoga parviflora</i> growing in the selenium-rich ore area of Enshi, Hubei Province, China. This strain can grow at pH levels of 5.0–10.0 and temperatures o...
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| Main Authors: | , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
MDPI AG
2025-07-01
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| Series: | Microorganisms |
| Subjects: | |
| Online Access: | https://www.mdpi.com/2076-2607/13/7/1559 |
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| Summary: | ES5-4<sup>T</sup>, a Gram-positive, motile, aerobic, and rod-shaped strain, was isolated from the rhizosphere of <i>Galinsoga parviflora</i> growing in the selenium-rich ore area of Enshi, Hubei Province, China. This strain can grow at pH levels of 5.0–10.0 and temperatures of 4–42 °C, with optimal growth at pH 7.0 and 28 °C. It was found to resist NaCl up to 5% (<i>w</i>/<i>v</i>), with an optimal growth condition of 0.5–1.0%. The strain exhibited tolerance to selenite (Se<sup>4+</sup>) concentrations up to 5000 mg/L. The major fatty acids of the ES5-4<sup>T</sup> strain were anteiso-C<sub>15:0</sub> (46.5%) and C<sub>16:0</sub> (21.7%), its predominant respiratory quinone was MK-7, and its polar lipids included diphosphatidylglycerol (DPG), phosphatidylethanolamine (PE), phosphatidylglycerol (PG), and an unidentified phospholipid (PL). The presence of the 16S rRNA gene sequence implies that ES5-4<sup>T</sup> belongs to a member of the genus <i>Paenibacillus</i>, with the highest sequence similarity of 98.4% to <i>Paenibacillus pabuli</i> NBRC 13638<sup>T</sup>. The bac120 tree also confirmed that the strain is within the genus <i>Paenibacillus</i>. The average nucleotide identity (ANI) and digital DNA–DNA hybridization (dDDH) values between ES5-4<sup>T</sup> and closely related members of the genus <i>Paenibacillus</i> were all below the cutoff levels of 95–96% and 70%, respectively. Based on a polyphasic approach, including phenotypic, chemotaxonomic, and phylogenetic analyses, the ES5-4<sup>T</sup> strain is proposed as a novel species of the genus <i>Paenibacillus</i>, for which the name <i>Paenibacillus hubeiensis</i> sp. nov. is proposed. This type strain is designated as ES5-4<sup>T</sup> (=GDMCC 1.3540<sup>T</sup> = KCTC 43478<sup>T</sup>). |
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| ISSN: | 2076-2607 |