Mapping pesticide-induced metabolic alterations in human gut bacteria

Abstract Pesticides can modulate gut microbiota composition, but their specific effects on it remain largely elusive. In our study, we show that pesticides inhibit or promote the growth of various gut microbial species and can be accumulated to prolong their presence in the host. Pesticide exposure...

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Main Authors: Li Chen, Hong Yan, Shanshan Di, Chao Guo, Huan Zhang, Shiqi Zhang, Andrew Gold, Yu Wang, Ming Hu, Dayong Wu, Caroline H. Johnson, Xinquan Wang, Jiangjiang Zhu
Format: Article
Language:English
Published: Nature Portfolio 2025-05-01
Series:Nature Communications
Online Access:https://doi.org/10.1038/s41467-025-59747-6
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author Li Chen
Hong Yan
Shanshan Di
Chao Guo
Huan Zhang
Shiqi Zhang
Andrew Gold
Yu Wang
Ming Hu
Dayong Wu
Caroline H. Johnson
Xinquan Wang
Jiangjiang Zhu
author_facet Li Chen
Hong Yan
Shanshan Di
Chao Guo
Huan Zhang
Shiqi Zhang
Andrew Gold
Yu Wang
Ming Hu
Dayong Wu
Caroline H. Johnson
Xinquan Wang
Jiangjiang Zhu
author_sort Li Chen
collection DOAJ
description Abstract Pesticides can modulate gut microbiota composition, but their specific effects on it remain largely elusive. In our study, we show that pesticides inhibit or promote the growth of various gut microbial species and can be accumulated to prolong their presence in the host. Pesticide exposure also induces significant alterations in gut bacterial metabolism, as reflected by changes in hundreds of metabolites. We generate a pesticide-gut microbiota-metabolite network that not only reveals pesticide-sensitive gut bacteria species but also reports specific metabolic changes in 306 pesticide-gut microbiota pairs. Using an in vivo mouse model, we further demonstrate the interactions of a representative pesticide-gut microbiota pair and verify the inflammation-inducing effects of pesticide exposure on the host, mediated by microbially dysregulated lipid metabolism. Taken together, our findings generate a comprehensive atlas of pesticide-gut microbiota-metabolite interactions atlas and shed light on the molecular mechanisms by which pesticides affect host health via gut microbiota-modulated metabolism.
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spelling doaj-art-478a64b8a15546408235aa4da2f8d2942025-08-20T01:49:48ZengNature PortfolioNature Communications2041-17232025-05-0116111510.1038/s41467-025-59747-6Mapping pesticide-induced metabolic alterations in human gut bacteriaLi Chen0Hong Yan1Shanshan Di2Chao Guo3Huan Zhang4Shiqi Zhang5Andrew Gold6Yu Wang7Ming Hu8Dayong Wu9Caroline H. Johnson10Xinquan Wang11Jiangjiang Zhu12Human Nutrition Program, Department of Human Sciences, The Ohio State UniversityDepartment of Environmental Health Sciences, Yale School of Public HealthState Key Laboratory of Agricultural Products Safety/ Key Laboratory of Detection for Pesticide Residues and Control of Zhejiang, Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural SciencesHuman Nutrition Program, Department of Human Sciences, The Ohio State UniversityHuman Nutrition Program, Department of Human Sciences, The Ohio State UniversityHuman Nutrition Program, Department of Human Sciences, The Ohio State UniversityHuman Nutrition Program, Department of Human Sciences, The Ohio State UniversityDepartment of Physiology, Johns Hopkins University School of MedicineHuman Nutrition Program, Department of Human Sciences, The Ohio State UniversityDepartment of Cancer Biology and Genetics, The Ohio State University College of MedicineDepartment of Environmental Health Sciences, Yale School of Public HealthState Key Laboratory of Agricultural Products Safety/ Key Laboratory of Detection for Pesticide Residues and Control of Zhejiang, Institute of Agro-product Safety and Nutrition, Zhejiang Academy of Agricultural SciencesHuman Nutrition Program, Department of Human Sciences, The Ohio State UniversityAbstract Pesticides can modulate gut microbiota composition, but their specific effects on it remain largely elusive. In our study, we show that pesticides inhibit or promote the growth of various gut microbial species and can be accumulated to prolong their presence in the host. Pesticide exposure also induces significant alterations in gut bacterial metabolism, as reflected by changes in hundreds of metabolites. We generate a pesticide-gut microbiota-metabolite network that not only reveals pesticide-sensitive gut bacteria species but also reports specific metabolic changes in 306 pesticide-gut microbiota pairs. Using an in vivo mouse model, we further demonstrate the interactions of a representative pesticide-gut microbiota pair and verify the inflammation-inducing effects of pesticide exposure on the host, mediated by microbially dysregulated lipid metabolism. Taken together, our findings generate a comprehensive atlas of pesticide-gut microbiota-metabolite interactions atlas and shed light on the molecular mechanisms by which pesticides affect host health via gut microbiota-modulated metabolism.https://doi.org/10.1038/s41467-025-59747-6
spellingShingle Li Chen
Hong Yan
Shanshan Di
Chao Guo
Huan Zhang
Shiqi Zhang
Andrew Gold
Yu Wang
Ming Hu
Dayong Wu
Caroline H. Johnson
Xinquan Wang
Jiangjiang Zhu
Mapping pesticide-induced metabolic alterations in human gut bacteria
Nature Communications
title Mapping pesticide-induced metabolic alterations in human gut bacteria
title_full Mapping pesticide-induced metabolic alterations in human gut bacteria
title_fullStr Mapping pesticide-induced metabolic alterations in human gut bacteria
title_full_unstemmed Mapping pesticide-induced metabolic alterations in human gut bacteria
title_short Mapping pesticide-induced metabolic alterations in human gut bacteria
title_sort mapping pesticide induced metabolic alterations in human gut bacteria
url https://doi.org/10.1038/s41467-025-59747-6
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