Molecular Characterization of Proteus spp. from Patients Admitted to Hospitals in Erbil City
The study was carried out to detect the identity of Proteus spp. isolated from various clinical specimens in Erbil City by polymerase chain reaction (PCR) technique. Specimens were of urine, wounds swabs, burn swabs, vaginal swabs, ear swabs, eye swabs, and sputum. Fifty-one Proteus isolates, (47) P...
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| Main Authors: | , |
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| Format: | Article |
| Language: | English |
| Published: |
Erbil Polytechnic University
2021-12-01
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| Series: | Polytechnic Journal |
| Subjects: | |
| Online Access: | https://polytechnic-journal.epu.edu.iq/home/vol11/iss2/15 |
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| Summary: | The study was carried out to detect the identity of Proteus spp. isolated from various clinical specimens
in Erbil City by polymerase chain reaction (PCR) technique. Specimens were of urine, wounds swabs,
burn swabs, vaginal swabs, ear swabs, eye swabs, and sputum. Fifty-one Proteus isolates, (47)
Proteus mirabilis, and (4) Proteus vulgaris, were undergone PCR assay using specific primers, targeting
the genes ureR and Urease C (ureC), that encode for urease enzyme as a virulence factor of Proteus
species. It was found that all isolates of Proteus mirabilis yielded positive result for ureR gene with an
amplicon length of 225 bp, as well as, all isolates of Proteus vulgaris exhibited positive PCR products
on gel for ureC gene with an amplicon length of 263 bp. Our results indicate that ureR and ureC based
PCR method seems to be an appropriate method for characterization of Proteus mirabilis and Proteus
vulgaris respectively. |
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| ISSN: | 2707-7799 |