Piezoelectricity of hexagonal boron nitrides improves bone tissue generation as tested on osteoblasts

Bone tissue, also known as bone, is a hard and specialized connective tissue consisting of various bone cells. Internally, it has a honeycomb-like matrix providing rigidity to the bone and a piezoelectric feature contributing to bone remodeling. Bone remodeling is a crucial process involving osteobl...

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Main Authors: Sevin Adiguzel, Nilay Cicek, Zehra Cobandede, Feray B. Misirlioglu, Hulya Yilmaz, Mustafa Culha
Format: Article
Language:English
Published: Beilstein-Institut 2025-07-01
Series:Beilstein Journal of Nanotechnology
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Online Access:https://doi.org/10.3762/bjnano.16.78
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author Sevin Adiguzel
Nilay Cicek
Zehra Cobandede
Feray B. Misirlioglu
Hulya Yilmaz
Mustafa Culha
author_facet Sevin Adiguzel
Nilay Cicek
Zehra Cobandede
Feray B. Misirlioglu
Hulya Yilmaz
Mustafa Culha
author_sort Sevin Adiguzel
collection DOAJ
description Bone tissue, also known as bone, is a hard and specialized connective tissue consisting of various bone cells. Internally, it has a honeycomb-like matrix providing rigidity to the bone and a piezoelectric feature contributing to bone remodeling. Bone remodeling is a crucial process involving osteoblastic replacement and resorption by osteoclastic cells to maintain structural integrity and mechanical properties of the bone tissue as it grows. However, in cases of fracture or degeneration, the natural self-regeneration process or inherent piezoelectricity of the body may not be sufficient to repair the damage. To address this, the use of piezoelectric nanomaterials (NMs) in bone tissue engineering was investigated. In this study, the influence of the piezoelectric hexagonal boron nitrides (hBNs) and barium titanate (BaTiO3) on human osteoblasts (HOb) was comparatively evaluated. The synthesized hBNs and purchased BaTiO3 were used after their full characterization by imaging and spectroscopic techniques. The piezoelectric behavior of both NMs was evaluated using piezoresponse force microscopy (PRFM). During in vitro studies, the piezoelectricity of the NMs was stimulated with ultrasound (US) exposure. The results showed that the NMs are not cytotoxic at the concentrations tested and the migration ability and calcium deposit formation of the cells treated with the NMs and upon US exposure were significantly increased. These results demonstrate that the hBNs have the potential to accelerate bone tissue regeneration and promote bone healing. These findings offer a promising avenue for developing new therapies for bone-related injuries and conditions requiring significant bone remodeling.
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spelling doaj-art-47886f5558844fbbbe8d6c1b0b2ffa1e2025-08-20T02:40:35ZengBeilstein-InstitutBeilstein Journal of Nanotechnology2190-42862025-07-011611068108110.3762/bjnano.16.782190-4286-16-78Piezoelectricity of hexagonal boron nitrides improves bone tissue generation as tested on osteoblastsSevin Adiguzel0Nilay Cicek1Zehra Cobandede2Feray B. Misirlioglu3Hulya Yilmaz4Mustafa Culha5Sabanci University Nanotechnology Research and Application Center (SUNUM), Istanbul, Turkey Sabanci University Nanotechnology Research and Application Center (SUNUM), Istanbul, Turkey Sabanci University Nanotechnology Research and Application Center (SUNUM), Istanbul, Turkey Sabanci University Nanotechnology Research and Application Center (SUNUM), Istanbul, Turkey Sabanci University Nanotechnology Research and Application Center (SUNUM), Istanbul, Turkey Sabanci University Nanotechnology Research and Application Center (SUNUM), Istanbul, Turkey Bone tissue, also known as bone, is a hard and specialized connective tissue consisting of various bone cells. Internally, it has a honeycomb-like matrix providing rigidity to the bone and a piezoelectric feature contributing to bone remodeling. Bone remodeling is a crucial process involving osteoblastic replacement and resorption by osteoclastic cells to maintain structural integrity and mechanical properties of the bone tissue as it grows. However, in cases of fracture or degeneration, the natural self-regeneration process or inherent piezoelectricity of the body may not be sufficient to repair the damage. To address this, the use of piezoelectric nanomaterials (NMs) in bone tissue engineering was investigated. In this study, the influence of the piezoelectric hexagonal boron nitrides (hBNs) and barium titanate (BaTiO3) on human osteoblasts (HOb) was comparatively evaluated. The synthesized hBNs and purchased BaTiO3 were used after their full characterization by imaging and spectroscopic techniques. The piezoelectric behavior of both NMs was evaluated using piezoresponse force microscopy (PRFM). During in vitro studies, the piezoelectricity of the NMs was stimulated with ultrasound (US) exposure. The results showed that the NMs are not cytotoxic at the concentrations tested and the migration ability and calcium deposit formation of the cells treated with the NMs and upon US exposure were significantly increased. These results demonstrate that the hBNs have the potential to accelerate bone tissue regeneration and promote bone healing. These findings offer a promising avenue for developing new therapies for bone-related injuries and conditions requiring significant bone remodeling.https://doi.org/10.3762/bjnano.16.78bone healinghexagonal boron nitrideshuman osteoblastsnanomaterialspiezoelectricity
spellingShingle Sevin Adiguzel
Nilay Cicek
Zehra Cobandede
Feray B. Misirlioglu
Hulya Yilmaz
Mustafa Culha
Piezoelectricity of hexagonal boron nitrides improves bone tissue generation as tested on osteoblasts
Beilstein Journal of Nanotechnology
bone healing
hexagonal boron nitrides
human osteoblasts
nanomaterials
piezoelectricity
title Piezoelectricity of hexagonal boron nitrides improves bone tissue generation as tested on osteoblasts
title_full Piezoelectricity of hexagonal boron nitrides improves bone tissue generation as tested on osteoblasts
title_fullStr Piezoelectricity of hexagonal boron nitrides improves bone tissue generation as tested on osteoblasts
title_full_unstemmed Piezoelectricity of hexagonal boron nitrides improves bone tissue generation as tested on osteoblasts
title_short Piezoelectricity of hexagonal boron nitrides improves bone tissue generation as tested on osteoblasts
title_sort piezoelectricity of hexagonal boron nitrides improves bone tissue generation as tested on osteoblasts
topic bone healing
hexagonal boron nitrides
human osteoblasts
nanomaterials
piezoelectricity
url https://doi.org/10.3762/bjnano.16.78
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