Antibiotics Resistance and Biofilm Formation Capacity of Staphylococcus spp. Strains Isolated from Surfaces and Medicotechnical Materials
Staphylococcus spp. is most often implicated in nosocomial infections. The objective of this study is to evaluate the susceptibility to antibiotics and the biofilm formation capacity of staphylococci species isolated from surfaces and medicotechnical materials at the university hospital center of Ab...
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| Format: | Article |
| Language: | English |
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Wiley
2020-01-01
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| Series: | International Journal of Microbiology |
| Online Access: | http://dx.doi.org/10.1155/2020/6512106 |
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| author | Akim Socohou Haziz Sina Cyriaque Degbey Chimène Nanoukon Kamirou Chabi-Sika Hélène Ahouandjinou Halfane Lehmane Farid Baba-Moussa Lamine Baba-Moussa |
| author_facet | Akim Socohou Haziz Sina Cyriaque Degbey Chimène Nanoukon Kamirou Chabi-Sika Hélène Ahouandjinou Halfane Lehmane Farid Baba-Moussa Lamine Baba-Moussa |
| author_sort | Akim Socohou |
| collection | DOAJ |
| description | Staphylococcus spp. is most often implicated in nosocomial infections. The objective of this study is to evaluate the susceptibility to antibiotics and the biofilm formation capacity of staphylococci species isolated from surfaces and medicotechnical materials at the university hospital center of Abomey-Calavi/Sô-Ava in Benin. Samples were collected according to ISO/DIS14698-1 standard from the surfaces and medicotechnical materials by the dry swab method. The isolation of Staphylococcus strains was performed on Chapman agar, and their identification was performed using microscopic and biochemical methods. The susceptibility of Staphylococcus isolates to antibiotics was evaluated by the disc diffusion method according to EUCAST and CLSI recommendations. The biofilm formation was qualitatively assessed using microplates. Of the 128 surfaces and medicotechnical material samples analyzed, 77% were contaminated with Staphylococcus spp. Thirteen species of Staphylococcus were isolated in different proportions but the pediatric department was the most contaminated (33%) by S. aureus. Resistance to antibiotics considerably varies according to the species of Staphylococcus. However, antibiotics such as chloramphenicol and vancomycin are the most effective on S. aureus, whereas coagulase-negative staphylococci developed less resistance to gentamycin and ciprofloxacin. The biofilm test reveals that 37% of our isolated strains were biofilm formers. Although regular monitoring of hospital hygiene is crucial, the optimal use of antibiotics is a cornerstone of reducing antimicrobial resistance. |
| format | Article |
| id | doaj-art-4783f915c6db4f4592103f94b25d7e69 |
| institution | Kabale University |
| issn | 1687-918X 1687-9198 |
| language | English |
| publishDate | 2020-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | International Journal of Microbiology |
| spelling | doaj-art-4783f915c6db4f4592103f94b25d7e692025-02-03T01:04:03ZengWileyInternational Journal of Microbiology1687-918X1687-91982020-01-01202010.1155/2020/65121066512106Antibiotics Resistance and Biofilm Formation Capacity of Staphylococcus spp. Strains Isolated from Surfaces and Medicotechnical MaterialsAkim Socohou0Haziz Sina1Cyriaque Degbey2Chimène Nanoukon3Kamirou Chabi-Sika4Hélène Ahouandjinou5Halfane Lehmane6Farid Baba-Moussa7Lamine Baba-Moussa8Laboratoire de Biologie et de Typage Moléculaire en Microbiologie, Département de Biochimie et de Biologie Cellulaire, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, Cotonou 05 BP 1604, BeninLaboratoire de Biologie et de Typage Moléculaire en Microbiologie, Département de Biochimie et de Biologie Cellulaire, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, Cotonou 05 BP 1604, BeninInstitut Régional de Santé Publique de Ouidah, Université d’Abomey-Calavi, Ouidah BP 384, BeninLaboratoire de Biologie et de Typage Moléculaire en Microbiologie, Département de Biochimie et de Biologie Cellulaire, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, Cotonou 05 BP 1604, BeninLaboratoire de Biologie et de Typage Moléculaire en Microbiologie, Département de Biochimie et de Biologie Cellulaire, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, Cotonou 05 BP 1604, BeninLaboratoire de Microbiologie et des Technologies Alimentaires, Département de Biologie Végétale, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, Cotonou 01 BP 526, BeninLaboratoire de Biologie et de Typage Moléculaire en Microbiologie, Département de Biochimie et de Biologie Cellulaire, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, Cotonou 05 BP 1604, BeninLaboratoire de Microbiologie et des Technologies Alimentaires, Département de Biologie Végétale, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, Cotonou 01 BP 526, BeninLaboratoire de Biologie et de Typage Moléculaire en Microbiologie, Département de Biochimie et de Biologie Cellulaire, Faculté des Sciences et Techniques, Université d’Abomey-Calavi, Cotonou 05 BP 1604, BeninStaphylococcus spp. is most often implicated in nosocomial infections. The objective of this study is to evaluate the susceptibility to antibiotics and the biofilm formation capacity of staphylococci species isolated from surfaces and medicotechnical materials at the university hospital center of Abomey-Calavi/Sô-Ava in Benin. Samples were collected according to ISO/DIS14698-1 standard from the surfaces and medicotechnical materials by the dry swab method. The isolation of Staphylococcus strains was performed on Chapman agar, and their identification was performed using microscopic and biochemical methods. The susceptibility of Staphylococcus isolates to antibiotics was evaluated by the disc diffusion method according to EUCAST and CLSI recommendations. The biofilm formation was qualitatively assessed using microplates. Of the 128 surfaces and medicotechnical material samples analyzed, 77% were contaminated with Staphylococcus spp. Thirteen species of Staphylococcus were isolated in different proportions but the pediatric department was the most contaminated (33%) by S. aureus. Resistance to antibiotics considerably varies according to the species of Staphylococcus. However, antibiotics such as chloramphenicol and vancomycin are the most effective on S. aureus, whereas coagulase-negative staphylococci developed less resistance to gentamycin and ciprofloxacin. The biofilm test reveals that 37% of our isolated strains were biofilm formers. Although regular monitoring of hospital hygiene is crucial, the optimal use of antibiotics is a cornerstone of reducing antimicrobial resistance.http://dx.doi.org/10.1155/2020/6512106 |
| spellingShingle | Akim Socohou Haziz Sina Cyriaque Degbey Chimène Nanoukon Kamirou Chabi-Sika Hélène Ahouandjinou Halfane Lehmane Farid Baba-Moussa Lamine Baba-Moussa Antibiotics Resistance and Biofilm Formation Capacity of Staphylococcus spp. Strains Isolated from Surfaces and Medicotechnical Materials International Journal of Microbiology |
| title | Antibiotics Resistance and Biofilm Formation Capacity of Staphylococcus spp. Strains Isolated from Surfaces and Medicotechnical Materials |
| title_full | Antibiotics Resistance and Biofilm Formation Capacity of Staphylococcus spp. Strains Isolated from Surfaces and Medicotechnical Materials |
| title_fullStr | Antibiotics Resistance and Biofilm Formation Capacity of Staphylococcus spp. Strains Isolated from Surfaces and Medicotechnical Materials |
| title_full_unstemmed | Antibiotics Resistance and Biofilm Formation Capacity of Staphylococcus spp. Strains Isolated from Surfaces and Medicotechnical Materials |
| title_short | Antibiotics Resistance and Biofilm Formation Capacity of Staphylococcus spp. Strains Isolated from Surfaces and Medicotechnical Materials |
| title_sort | antibiotics resistance and biofilm formation capacity of staphylococcus spp strains isolated from surfaces and medicotechnical materials |
| url | http://dx.doi.org/10.1155/2020/6512106 |
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