High Antennal Expression of <i>CYP6K1</i> and <i>CYP4V2</i> Participate in the Recognition of Alarm Pheromones by <i>Solenopsis invicta</i> Buren
Insects have highly developed olfactory systems in which cytochrome P450s (CYPs) were involved as odor-degrading enzymes throughout the olfactory recognition of odor compounds by insects to avoid continuous stimulation of signaling molecules and thus damage to the olfactory nervous. To understand wh...
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MDPI AG
2025-01-01
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| Series: | Insects |
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| author | Xinyi Jiang Jiacheng Shen Peng Lin Youming Hou |
| author_facet | Xinyi Jiang Jiacheng Shen Peng Lin Youming Hou |
| author_sort | Xinyi Jiang |
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| description | Insects have highly developed olfactory systems in which cytochrome P450s (CYPs) were involved as odor-degrading enzymes throughout the olfactory recognition of odor compounds by insects to avoid continuous stimulation of signaling molecules and thus damage to the olfactory nervous. To understand whether the highly expressed CYPs in the antennae play an olfactory function in <i>Solenopsis invicta</i> worker, in this study, we find six highly expressed antennal CYPs from the transcriptome of <i>S. invicta</i>. Multiple sequence alignment and phylogenetic analysis divided them into two families: the CYP3 family (<i>SinvCYP6K1</i>, <i>SinvCYP6K1-1</i>) and the CYP4 family (<i>SinvCYP4C1</i>, <i>SinvCYP4C1-1</i>, <i>SinvCYP4C1-2</i>, <i>SinvCYP4V2</i>). The expression patterns of these six CYPs were analyzed by RT-qPCR, which revealed that <i>SinvCYP6K1</i> and <i>SinvCYP4V2</i> were only highly expressed in the antennae of adult workers. The expression of <i>SinvCYP6K1</i> and <i>SinvCYP4V2</i> in workers was markedly diminished after feeding with dsRNA. The electroantennography (EAG) assay demonstrated that the silencing of either <i>SinvCYP6K1</i> or <i>SinvCYP4V2</i> resulted in a notable reduction in the EAG response of workers to 2-ethyl-3,6(5)-dimethylpyrazine (EDMP). Furthermore, the trajectory behavior assay showed that the worker’s range and speed of movement in response to EDMP significant decreased after the silencing of <i>SinvCYP6K1</i> and <i>SinvCYP4V2</i>. The findings indicated that both <i>SinvCYP6K1</i> and <i>SinvCYP4V2</i> were implicated in the recognition of EDMP by <i>S. invicta</i>. |
| format | Article |
| id | doaj-art-4780d729ed29451a9c4bc17e610d6437 |
| institution | Kabale University |
| issn | 2075-4450 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | MDPI AG |
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| series | Insects |
| spelling | doaj-art-4780d729ed29451a9c4bc17e610d64372025-01-24T13:35:41ZengMDPI AGInsects2075-44502025-01-011614310.3390/insects16010043High Antennal Expression of <i>CYP6K1</i> and <i>CYP4V2</i> Participate in the Recognition of Alarm Pheromones by <i>Solenopsis invicta</i> BurenXinyi Jiang0Jiacheng Shen1Peng Lin2Youming Hou3State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaState Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops, Fujian Agriculture and Forestry University, Fuzhou 350002, ChinaInsects have highly developed olfactory systems in which cytochrome P450s (CYPs) were involved as odor-degrading enzymes throughout the olfactory recognition of odor compounds by insects to avoid continuous stimulation of signaling molecules and thus damage to the olfactory nervous. To understand whether the highly expressed CYPs in the antennae play an olfactory function in <i>Solenopsis invicta</i> worker, in this study, we find six highly expressed antennal CYPs from the transcriptome of <i>S. invicta</i>. Multiple sequence alignment and phylogenetic analysis divided them into two families: the CYP3 family (<i>SinvCYP6K1</i>, <i>SinvCYP6K1-1</i>) and the CYP4 family (<i>SinvCYP4C1</i>, <i>SinvCYP4C1-1</i>, <i>SinvCYP4C1-2</i>, <i>SinvCYP4V2</i>). The expression patterns of these six CYPs were analyzed by RT-qPCR, which revealed that <i>SinvCYP6K1</i> and <i>SinvCYP4V2</i> were only highly expressed in the antennae of adult workers. The expression of <i>SinvCYP6K1</i> and <i>SinvCYP4V2</i> in workers was markedly diminished after feeding with dsRNA. The electroantennography (EAG) assay demonstrated that the silencing of either <i>SinvCYP6K1</i> or <i>SinvCYP4V2</i> resulted in a notable reduction in the EAG response of workers to 2-ethyl-3,6(5)-dimethylpyrazine (EDMP). Furthermore, the trajectory behavior assay showed that the worker’s range and speed of movement in response to EDMP significant decreased after the silencing of <i>SinvCYP6K1</i> and <i>SinvCYP4V2</i>. The findings indicated that both <i>SinvCYP6K1</i> and <i>SinvCYP4V2</i> were implicated in the recognition of EDMP by <i>S. invicta</i>.https://www.mdpi.com/2075-4450/16/1/43red imported fire antalarm pheromonecytochrome P450sRNA interferenceelectroantennography |
| spellingShingle | Xinyi Jiang Jiacheng Shen Peng Lin Youming Hou High Antennal Expression of <i>CYP6K1</i> and <i>CYP4V2</i> Participate in the Recognition of Alarm Pheromones by <i>Solenopsis invicta</i> Buren Insects red imported fire ant alarm pheromone cytochrome P450s RNA interference electroantennography |
| title | High Antennal Expression of <i>CYP6K1</i> and <i>CYP4V2</i> Participate in the Recognition of Alarm Pheromones by <i>Solenopsis invicta</i> Buren |
| title_full | High Antennal Expression of <i>CYP6K1</i> and <i>CYP4V2</i> Participate in the Recognition of Alarm Pheromones by <i>Solenopsis invicta</i> Buren |
| title_fullStr | High Antennal Expression of <i>CYP6K1</i> and <i>CYP4V2</i> Participate in the Recognition of Alarm Pheromones by <i>Solenopsis invicta</i> Buren |
| title_full_unstemmed | High Antennal Expression of <i>CYP6K1</i> and <i>CYP4V2</i> Participate in the Recognition of Alarm Pheromones by <i>Solenopsis invicta</i> Buren |
| title_short | High Antennal Expression of <i>CYP6K1</i> and <i>CYP4V2</i> Participate in the Recognition of Alarm Pheromones by <i>Solenopsis invicta</i> Buren |
| title_sort | high antennal expression of i cyp6k1 i and i cyp4v2 i participate in the recognition of alarm pheromones by i solenopsis invicta i buren |
| topic | red imported fire ant alarm pheromone cytochrome P450s RNA interference electroantennography |
| url | https://www.mdpi.com/2075-4450/16/1/43 |
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