Effect of Different Concentrations of Topically Applied Boswellia Serrata Extract on Excisional Wound Healing in Rats

Wound is known as one of the serious health issue. Boswellia serrata (BS) contains a wide range of phytochemicals with different pharmacological properties. The study aimed to assess the wound healing potential of Boswellia Serrata Extract (BSE) against an experimental model of excision wound. A to...

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Main Authors: Bushra Hassan Marouf, Dana Omer Ismaeel, Goran Mohammad Raouf Abdulqader
Format: Article
Language:English
Published: College of Pharmacy University of Baghdad 2025-03-01
Series:Iraqi Journal of Pharmaceutical Sciences
Subjects:
Online Access:https://bijps.uobaghdad.edu.iq/index.php/bijps/article/view/3069
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author Bushra Hassan Marouf
Dana Omer Ismaeel
Goran Mohammad Raouf Abdulqader
author_facet Bushra Hassan Marouf
Dana Omer Ismaeel
Goran Mohammad Raouf Abdulqader
author_sort Bushra Hassan Marouf
collection DOAJ
description Wound is known as one of the serious health issue. Boswellia serrata (BS) contains a wide range of phytochemicals with different pharmacological properties. The study aimed to assess the wound healing potential of Boswellia Serrata Extract (BSE) against an experimental model of excision wound. A total of 45 rats in five equal groups were used in this investigation. The wound was created in the cervical dorsal region of each rat as a two-symmetrical circle pattern. All groups were being treated as follow: control group with ointment base, β-Sitosterol group with 0.25% w/w β-Sitosterol-containing ointment, three BSE ointment groups; treated with BSE ointment containing 5%, 10%, and 20% boswellic acid (BA) respectively. The wounds were followed up for 15 days. On days 5, 10, and 15, three rats from each group were sacrificed, the wounds and scars were sent for histopathological study. The ointments containing 5% and 10% BA exerted a significantly increased wound contraction rate, lesser surface area and shorter re-epithelialization period in the macroscopical analysis in compare to the control group and the β-Sitosterol group. However, the microscopical results exhibited a significant healing progress in a concentration dependent-manner. This was reflected by formation of clear dermal granulation tissue, collagen deposition and angiogenesis in quantitative histological evaluation. In conclusion, this study provided a macroscopical and microscopical evidences for a potential wound healing effect of the topical application of BSE in concentration dependent manner. Although the highest BA concentration in macroscopical analysis exhibited lesser efficacy.
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spelling doaj-art-476e486156b34bc2bbc2a37c879c496f2025-08-20T02:10:17ZengCollege of Pharmacy University of BaghdadIraqi Journal of Pharmaceutical Sciences1683-35972521-35122025-03-0134110.31351/vol34iss1pp72-84Effect of Different Concentrations of Topically Applied Boswellia Serrata Extract on Excisional Wound Healing in RatsBushra Hassan Marouf0Dana Omer Ismaeel1https://orcid.org/0000-0003-0221-1541Goran Mohammad Raouf Abdulqader2Department of Pharmacology and Toxicology-College of Pharmacy-University of Sulaimani-Sulaimani-Kurdistan Region-IraqDepartment of Surgery and Theriogenology, College of Veterinary Medicine, University of Sulaimani, Sulaimani, Kurdistan Region, Iraq.3Department of Pathology and Forensic Pathology, College of Medicine, University of Sulaimani, Sulaimani, Kurdistan Region, Iraq. Wound is known as one of the serious health issue. Boswellia serrata (BS) contains a wide range of phytochemicals with different pharmacological properties. The study aimed to assess the wound healing potential of Boswellia Serrata Extract (BSE) against an experimental model of excision wound. A total of 45 rats in five equal groups were used in this investigation. The wound was created in the cervical dorsal region of each rat as a two-symmetrical circle pattern. All groups were being treated as follow: control group with ointment base, β-Sitosterol group with 0.25% w/w β-Sitosterol-containing ointment, three BSE ointment groups; treated with BSE ointment containing 5%, 10%, and 20% boswellic acid (BA) respectively. The wounds were followed up for 15 days. On days 5, 10, and 15, three rats from each group were sacrificed, the wounds and scars were sent for histopathological study. The ointments containing 5% and 10% BA exerted a significantly increased wound contraction rate, lesser surface area and shorter re-epithelialization period in the macroscopical analysis in compare to the control group and the β-Sitosterol group. However, the microscopical results exhibited a significant healing progress in a concentration dependent-manner. This was reflected by formation of clear dermal granulation tissue, collagen deposition and angiogenesis in quantitative histological evaluation. In conclusion, this study provided a macroscopical and microscopical evidences for a potential wound healing effect of the topical application of BSE in concentration dependent manner. Although the highest BA concentration in macroscopical analysis exhibited lesser efficacy. https://bijps.uobaghdad.edu.iq/index.php/bijps/article/view/3069boswellic acidepithelializationexcision woundwound healing
spellingShingle Bushra Hassan Marouf
Dana Omer Ismaeel
Goran Mohammad Raouf Abdulqader
Effect of Different Concentrations of Topically Applied Boswellia Serrata Extract on Excisional Wound Healing in Rats
Iraqi Journal of Pharmaceutical Sciences
boswellic acid
epithelialization
excision wound
wound healing
title Effect of Different Concentrations of Topically Applied Boswellia Serrata Extract on Excisional Wound Healing in Rats
title_full Effect of Different Concentrations of Topically Applied Boswellia Serrata Extract on Excisional Wound Healing in Rats
title_fullStr Effect of Different Concentrations of Topically Applied Boswellia Serrata Extract on Excisional Wound Healing in Rats
title_full_unstemmed Effect of Different Concentrations of Topically Applied Boswellia Serrata Extract on Excisional Wound Healing in Rats
title_short Effect of Different Concentrations of Topically Applied Boswellia Serrata Extract on Excisional Wound Healing in Rats
title_sort effect of different concentrations of topically applied boswellia serrata extract on excisional wound healing in rats
topic boswellic acid
epithelialization
excision wound
wound healing
url https://bijps.uobaghdad.edu.iq/index.php/bijps/article/view/3069
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