Interaction Effect of PEG6000 and Brassinosteroid on Biochemical Characteristics of in Vitro Date Palm (Barhi cultivar)
Somatic embryogenesis is an essential method of date palm micropropagation that produces large biomass of plants. The callus is the primary plant tissue that goes through the indirect propagation way, which is the mainstay in plant formation that resists environmental stresses. Polyethylene Glycol (...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
University of Basrah
2025-08-01
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| Series: | Maǧallaẗ al-baṣraẗ al-ʻulūm al-zirāʻiyyaẗ |
| Subjects: | |
| Online Access: | https://bjas.bajas.edu.iq/index.php/bjas/article/view/2646 |
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| Summary: | Somatic embryogenesis is an essential method of date palm micropropagation that produces large biomass of plants. The callus is the primary plant tissue that goes through the indirect propagation way, which is the mainstay in plant formation that resists environmental stresses. Polyethylene Glycol (PEG) is used in vitro experiments to induce osmotic stress, while Brassinosteroid is a promising hormone used to overcome or reduce water stress in a nutrition medium. The experiment aims to study the effect of PEG and BR on the biochemical characteristics of date palm tissues. Four concentrations of PEG (0, 10, 20, and 30g.L-1) were combined with four concentrations of the hormone (0, 0.2, 0.5, and 1µmol.L-1) in the nutritional media of date palm tissues at the callus and embryo stages. The results showed significant increases in proline, catalase enzyme, and membrane stability index tissue content, which are stress resistance indicators of plant tissues. Conversely, there is a decrease in the MDA content, conceded as an indicator of plant degradation by stress. These are valuable results because they prove the essential role of BR in the stress resistance of plant tissues, and by increasing this resistance; a strong plant can be produced that is resistant to environmental stresses.
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| ISSN: | 1814-5868 2520-0860 |