Inhibition of Ultraviolet B-induced Apoptosis in Fibroblasts by Human Umbilical Cord Blood Mesenchymal Stem Cell Conditioned Media via the Phosphatidylinositol-3-Kinase/Akt Pathway
Background: Ultraviolet B (UVB) irradiation induces photoaging and apoptosis in various cell types. Inhibition of UVB-induced apoptotic pathways has been explored in different apoptotic cascades. Conditioned media from human umbilical cord blood mesenchymal stem cells (CM-hUCB-MSC) contain important...
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Shiraz University of Medical Sciences
2025-01-01
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| Series: | Iranian Journal of Medical Sciences |
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| Online Access: | https://ijms.sums.ac.ir/article_50416_52b8ced2b8913447d1ebc69d6a8afe9a.pdf |
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| author | Ferry Sandra Dian Andriani Ratna Dewi I Nyoman Mantik Astawa Wimpie Pangkahila Angliana Chouw Maria Celinna Nurrani Mustika Dewi |
| author_facet | Ferry Sandra Dian Andriani Ratna Dewi I Nyoman Mantik Astawa Wimpie Pangkahila Angliana Chouw Maria Celinna Nurrani Mustika Dewi |
| author_sort | Ferry Sandra |
| collection | DOAJ |
| description | Background: Ultraviolet B (UVB) irradiation induces photoaging and apoptosis in various cell types. Inhibition of UVB-induced apoptotic pathways has been explored in different apoptotic cascades. Conditioned media from human umbilical cord blood mesenchymal stem cells (CM-hUCB-MSC) contain important substances for cell regeneration. However, the potential of CM-hUCB-MSC in preventing UVB-induced apoptosis has not been clearly elucidated. Therefore, the current research was conducted to investigate the potential of CM-hUCB-MSC in inhibiting UVB-induced apoptosis and its role in the antiapoptotic signaling pathway.Methods: An experimental in vitro study was conducted at PT. Prodia StemCell Indonesia, Jakarta, Indonesia, 2019-2022. Initially, hUCB-MSCs were isolated and cultured to produce CM-hUCB-MSC. NIH3T3 cells were pretreated with/without 50 μM LY294002, treated with/without 10% CM-hUCB-MSC, and then irradiated with/without UVB. Subsequently, the cells were analyzed using sub-G1, immunofluorescence, and immunoblotting assays. One-way analysis of variance (ANOVA) was used for data analysis, followed by Tukey’s honest significant difference (HSD) test or the Kruskal-Wallis test, followed by the Dunn-Bonferroni test using IBM SPSS Statistics software version 21. Statistical significance was determined at P<0.05.Results: CM-hUCB-MSC significantly inhibited UVB-induced apoptosis in NIH3T3 cells (P=0.002, Dunn-Bonferroni test). CM-hUCB-MSC significantly induced Akt phosphorylation at Ser 473 in UVB-irradiated NIH3T3 cells (P<0.001, Tukey’s HSD test). The CM-hUCB-MSC-induced phosphorylation of Akt was significantly inhibited by LY294002 (P<0.001, Tukey’s HSD test).Conclusion: Taken together, it can be concluded that CM-hUCB-MSC inhibits UVB-induced NIH3T3 cell apoptosis via the activation of phosphatidylinositol-3-kinase (PI3K)/Akt signaling cascades. |
| format | Article |
| id | doaj-art-4753dd89f8584a8da1c969edd7b6e1ab |
| institution | Kabale University |
| issn | 0253-0716 1735-3688 |
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| publishDate | 2025-01-01 |
| publisher | Shiraz University of Medical Sciences |
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| series | Iranian Journal of Medical Sciences |
| spelling | doaj-art-4753dd89f8584a8da1c969edd7b6e1ab2025-02-11T07:30:14ZengShiraz University of Medical SciencesIranian Journal of Medical Sciences0253-07161735-36882025-01-01501445310.30476/ijms.2024.100856.334250416Inhibition of Ultraviolet B-induced Apoptosis in Fibroblasts by Human Umbilical Cord Blood Mesenchymal Stem Cell Conditioned Media via the Phosphatidylinositol-3-Kinase/Akt PathwayFerry Sandra0Dian Andriani Ratna Dewi1I Nyoman Mantik Astawa2Wimpie Pangkahila3Angliana Chouw4Maria Celinna5Nurrani Mustika Dewi6Department of Biochemistry and Molecular Biology, Division of Oral Biology, Faculty of Dentistry, Universitas Trisakti, Jakarta, IndonesiaGatot Soebroto Army Hospital, Jakarta, IndonesiaFaculty of Veterinary Medicine, Universitas Udayana, Denpasar, IndonesiaDepartment of Andrology and Sexology, Faculty of Medicine, Universitas Udayana, Denpasar, IndonesiaPT Prodia StemCell Indonesia, Jakarta, IndonesiaThe Prodia Education and Research Institute, Jakarta, IndonesiaThe Prodia Education and Research Institute, Jakarta, IndonesiaBackground: Ultraviolet B (UVB) irradiation induces photoaging and apoptosis in various cell types. Inhibition of UVB-induced apoptotic pathways has been explored in different apoptotic cascades. Conditioned media from human umbilical cord blood mesenchymal stem cells (CM-hUCB-MSC) contain important substances for cell regeneration. However, the potential of CM-hUCB-MSC in preventing UVB-induced apoptosis has not been clearly elucidated. Therefore, the current research was conducted to investigate the potential of CM-hUCB-MSC in inhibiting UVB-induced apoptosis and its role in the antiapoptotic signaling pathway.Methods: An experimental in vitro study was conducted at PT. Prodia StemCell Indonesia, Jakarta, Indonesia, 2019-2022. Initially, hUCB-MSCs were isolated and cultured to produce CM-hUCB-MSC. NIH3T3 cells were pretreated with/without 50 μM LY294002, treated with/without 10% CM-hUCB-MSC, and then irradiated with/without UVB. Subsequently, the cells were analyzed using sub-G1, immunofluorescence, and immunoblotting assays. One-way analysis of variance (ANOVA) was used for data analysis, followed by Tukey’s honest significant difference (HSD) test or the Kruskal-Wallis test, followed by the Dunn-Bonferroni test using IBM SPSS Statistics software version 21. Statistical significance was determined at P<0.05.Results: CM-hUCB-MSC significantly inhibited UVB-induced apoptosis in NIH3T3 cells (P=0.002, Dunn-Bonferroni test). CM-hUCB-MSC significantly induced Akt phosphorylation at Ser 473 in UVB-irradiated NIH3T3 cells (P<0.001, Tukey’s HSD test). The CM-hUCB-MSC-induced phosphorylation of Akt was significantly inhibited by LY294002 (P<0.001, Tukey’s HSD test).Conclusion: Taken together, it can be concluded that CM-hUCB-MSC inhibits UVB-induced NIH3T3 cell apoptosis via the activation of phosphatidylinositol-3-kinase (PI3K)/Akt signaling cascades.https://ijms.sums.ac.ir/article_50416_52b8ced2b8913447d1ebc69d6a8afe9a.pdfultraviolet raysapoptosisculture media, conditionedmesenchymal stem cellsproto-oncogene proteins c-akt |
| spellingShingle | Ferry Sandra Dian Andriani Ratna Dewi I Nyoman Mantik Astawa Wimpie Pangkahila Angliana Chouw Maria Celinna Nurrani Mustika Dewi Inhibition of Ultraviolet B-induced Apoptosis in Fibroblasts by Human Umbilical Cord Blood Mesenchymal Stem Cell Conditioned Media via the Phosphatidylinositol-3-Kinase/Akt Pathway Iranian Journal of Medical Sciences ultraviolet rays apoptosis culture media, conditioned mesenchymal stem cells proto-oncogene proteins c-akt |
| title | Inhibition of Ultraviolet B-induced Apoptosis in Fibroblasts by Human Umbilical Cord Blood Mesenchymal Stem Cell Conditioned Media via the Phosphatidylinositol-3-Kinase/Akt Pathway |
| title_full | Inhibition of Ultraviolet B-induced Apoptosis in Fibroblasts by Human Umbilical Cord Blood Mesenchymal Stem Cell Conditioned Media via the Phosphatidylinositol-3-Kinase/Akt Pathway |
| title_fullStr | Inhibition of Ultraviolet B-induced Apoptosis in Fibroblasts by Human Umbilical Cord Blood Mesenchymal Stem Cell Conditioned Media via the Phosphatidylinositol-3-Kinase/Akt Pathway |
| title_full_unstemmed | Inhibition of Ultraviolet B-induced Apoptosis in Fibroblasts by Human Umbilical Cord Blood Mesenchymal Stem Cell Conditioned Media via the Phosphatidylinositol-3-Kinase/Akt Pathway |
| title_short | Inhibition of Ultraviolet B-induced Apoptosis in Fibroblasts by Human Umbilical Cord Blood Mesenchymal Stem Cell Conditioned Media via the Phosphatidylinositol-3-Kinase/Akt Pathway |
| title_sort | inhibition of ultraviolet b induced apoptosis in fibroblasts by human umbilical cord blood mesenchymal stem cell conditioned media via the phosphatidylinositol 3 kinase akt pathway |
| topic | ultraviolet rays apoptosis culture media, conditioned mesenchymal stem cells proto-oncogene proteins c-akt |
| url | https://ijms.sums.ac.ir/article_50416_52b8ced2b8913447d1ebc69d6a8afe9a.pdf |
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