A global view of protein expression in human cells, tissues, and organs
Abstract Defining the protein profiles of tissues and organs is critical to understanding the unique characteristics of the various cell types in the human body. In this study, we report on an anatomically comprehensive analysis of 4842 protein profiles in 48 human tissues and 45 human cell lines. A...
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| Main Authors: | , , , , , , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Springer Nature
2009-12-01
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| Series: | Molecular Systems Biology |
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| Online Access: | https://doi.org/10.1038/msb.2009.93 |
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| _version_ | 1849225758296244224 |
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| author | Fredrik Pontén Marcus Gry Linn Fagerberg Emma Lundberg Anna Asplund Lisa Berglund Per Oksvold Erik Björling Sophia Hober Caroline Kampf Sanjay Navani Peter Nilsson Jenny Ottosson Anja Persson Henrik Wernérus Kenneth Wester Mathias Uhlén |
| author_facet | Fredrik Pontén Marcus Gry Linn Fagerberg Emma Lundberg Anna Asplund Lisa Berglund Per Oksvold Erik Björling Sophia Hober Caroline Kampf Sanjay Navani Peter Nilsson Jenny Ottosson Anja Persson Henrik Wernérus Kenneth Wester Mathias Uhlén |
| author_sort | Fredrik Pontén |
| collection | DOAJ |
| description | Abstract Defining the protein profiles of tissues and organs is critical to understanding the unique characteristics of the various cell types in the human body. In this study, we report on an anatomically comprehensive analysis of 4842 protein profiles in 48 human tissues and 45 human cell lines. A detailed analysis of over 2 million manually annotated, high‐resolution, immunohistochemistry‐based images showed a high fraction (>65%) of expressed proteins in most cells and tissues, with very few proteins (<2%) detected in any single cell type. Similarly, confocal microscopy in three human cell lines detected expression of more than 70% of the analyzed proteins. Despite this ubiquitous expression, hierarchical clustering analysis, based on global protein expression patterns, shows that the analyzed cells can be still subdivided into groups according to the current concepts of histology and cellular differentiation. This study suggests that tissue specificity is achieved by precise regulation of protein levels in space and time, and that different tissues in the body acquire their unique characteristics by controlling not which proteins are expressed but how much of each is produced. |
| format | Article |
| id | doaj-art-4749a62b2e984395b4aaaad454dbaed4 |
| institution | Kabale University |
| issn | 1744-4292 |
| language | English |
| publishDate | 2009-12-01 |
| publisher | Springer Nature |
| record_format | Article |
| series | Molecular Systems Biology |
| spelling | doaj-art-4749a62b2e984395b4aaaad454dbaed42025-08-24T11:58:53ZengSpringer NatureMolecular Systems Biology1744-42922009-12-01511910.1038/msb.2009.93A global view of protein expression in human cells, tissues, and organsFredrik Pontén0Marcus Gry1Linn Fagerberg2Emma Lundberg3Anna Asplund4Lisa Berglund5Per Oksvold6Erik Björling7Sophia Hober8Caroline Kampf9Sanjay Navani10Peter Nilsson11Jenny Ottosson12Anja Persson13Henrik Wernérus14Kenneth Wester15Mathias Uhlén16Department of Genetics and Pathology, The Rudbeck Laboratory, Uppsala UniversityDepartment of Genetics and Pathology, The Rudbeck Laboratory, Uppsala UniversityDepartment of Proteomics, School of Biotechnology, AlbaNova University Center, Royal Institute of Technology (KTH)Department of Proteomics, School of Biotechnology, AlbaNova University Center, Royal Institute of Technology (KTH)Department of Genetics and Pathology, The Rudbeck Laboratory, Uppsala UniversityDepartment of Proteomics, School of Biotechnology, AlbaNova University Center, Royal Institute of Technology (KTH)Department of Proteomics, School of Biotechnology, AlbaNova University Center, Royal Institute of Technology (KTH)Department of Proteomics, School of Biotechnology, AlbaNova University Center, Royal Institute of Technology (KTH)Department of Proteomics, School of Biotechnology, AlbaNova University Center, Royal Institute of Technology (KTH)Department of Genetics and Pathology, The Rudbeck Laboratory, Uppsala UniversityLab SurgpathDepartment of Proteomics, School of Biotechnology, AlbaNova University Center, Royal Institute of Technology (KTH)Department of Proteomics, School of Biotechnology, AlbaNova University Center, Royal Institute of Technology (KTH)Department of Proteomics, School of Biotechnology, AlbaNova University Center, Royal Institute of Technology (KTH)Department of Proteomics, School of Biotechnology, AlbaNova University Center, Royal Institute of Technology (KTH)Department of Genetics and Pathology, The Rudbeck Laboratory, Uppsala UniversityDepartment of Proteomics, School of Biotechnology, AlbaNova University Center, Royal Institute of Technology (KTH)Abstract Defining the protein profiles of tissues and organs is critical to understanding the unique characteristics of the various cell types in the human body. In this study, we report on an anatomically comprehensive analysis of 4842 protein profiles in 48 human tissues and 45 human cell lines. A detailed analysis of over 2 million manually annotated, high‐resolution, immunohistochemistry‐based images showed a high fraction (>65%) of expressed proteins in most cells and tissues, with very few proteins (<2%) detected in any single cell type. Similarly, confocal microscopy in three human cell lines detected expression of more than 70% of the analyzed proteins. Despite this ubiquitous expression, hierarchical clustering analysis, based on global protein expression patterns, shows that the analyzed cells can be still subdivided into groups according to the current concepts of histology and cellular differentiation. This study suggests that tissue specificity is achieved by precise regulation of protein levels in space and time, and that different tissues in the body acquire their unique characteristics by controlling not which proteins are expressed but how much of each is produced.https://doi.org/10.1038/msb.2009.93antibody‐based analysisbioimagingglobal protein expressionimmunofluorescenceimmunohistochemistry |
| spellingShingle | Fredrik Pontén Marcus Gry Linn Fagerberg Emma Lundberg Anna Asplund Lisa Berglund Per Oksvold Erik Björling Sophia Hober Caroline Kampf Sanjay Navani Peter Nilsson Jenny Ottosson Anja Persson Henrik Wernérus Kenneth Wester Mathias Uhlén A global view of protein expression in human cells, tissues, and organs Molecular Systems Biology antibody‐based analysis bioimaging global protein expression immunofluorescence immunohistochemistry |
| title | A global view of protein expression in human cells, tissues, and organs |
| title_full | A global view of protein expression in human cells, tissues, and organs |
| title_fullStr | A global view of protein expression in human cells, tissues, and organs |
| title_full_unstemmed | A global view of protein expression in human cells, tissues, and organs |
| title_short | A global view of protein expression in human cells, tissues, and organs |
| title_sort | global view of protein expression in human cells tissues and organs |
| topic | antibody‐based analysis bioimaging global protein expression immunofluorescence immunohistochemistry |
| url | https://doi.org/10.1038/msb.2009.93 |
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