Experimental study of systemic hemostatic effects of fibrin monomer in inhibition of platelet aggregation function

Aim. The study objective was to examine fibrin monomer hemostatic effects in post-traumatic parenchymal hemorrhage in the setting of pharmacologically associated platelet aggregation inhibition.Materials and methods. In the in vivo experimental study on male rabbits, the hemostatic effects of fibrin...

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Main Authors: V. M. Vdovin, A. P. Momot, D. A. Orekhov, I. G. Tolstokorov, N. A. Lycheva, V. O. Shevchenko, I. I. Shakhmatov, V. O. Krasyukova, E. V. Fogt
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Language:English
Published: Siberian State Medical University (Tomsk) 2020-04-01
Series:Бюллетень сибирской медицины
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Online Access:https://bulletin.ssmu.ru/jour/article/view/2678
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author V. M. Vdovin
A. P. Momot
D. A. Orekhov
I. G. Tolstokorov
N. A. Lycheva
V. O. Shevchenko
I. I. Shakhmatov
V. O. Krasyukova
E. V. Fogt
author_facet V. M. Vdovin
A. P. Momot
D. A. Orekhov
I. G. Tolstokorov
N. A. Lycheva
V. O. Shevchenko
I. I. Shakhmatov
V. O. Krasyukova
E. V. Fogt
author_sort V. M. Vdovin
collection DOAJ
description Aim. The study objective was to examine fibrin monomer hemostatic effects in post-traumatic parenchymal hemorrhage in the setting of pharmacologically associated platelet aggregation inhibition.Materials and methods. In the in vivo experimental study on male rabbits, the hemostatic effects of fibrin monomer (FM) (0.25 mg/kg) were evaluated in comparison with tranexamic acid (TA) (15 mg/kg) in post-traumatic parenchymal hemorrhage against the background of preliminary inhibition of platelet aggregation function with acetylsalicylic acid (2.0 mg/kg) and clopidogrel (8.0 mg/kg). Volume and rate of blood loss were estimated, as well as the parameters of the hemostatic system.Results. It has been established that FM versus placebo when administered intravenously 1 hour before the injury can prevent severe bleeding associated with taking antiplatelet agents. The volume of blood loss after FM administration decreased in median by 6.0 times, the rate of blood loss – by 5.9 times, and when using TA – by 2.4 (РFM-TA < 0.02) and 4.8 times respectively. The hemostatic effects of TA were realized when the hemostatic balance was shifted towards the increased fibrin formation (an increase in the level of D-dimer in the blood plasma). The use of FM was not accompanied by any significant changes in the blood coagulation system.Conclusion. The fibrin monomer at a dose of 0.25 mg/kg i.v. is capable of preventing severe  posttraumatic parenchymal bleeding caused by the combined use of drugs with different antiplatelet action. The phenomenon is not completely clear and needs to be analyzed in further research. The mechanism of hemostatic effects associated with FM is currently being studied.
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spelling doaj-art-473f8d2e6a324c98a9412a463eea29ab2025-08-20T03:02:03ZengSiberian State Medical University (Tomsk)Бюллетень сибирской медицины1682-03631819-36842020-04-01191364210.20538/1682-0363-2020-1-36-421594Experimental study of systemic hemostatic effects of fibrin monomer in inhibition of platelet aggregation functionV. M. Vdovin0A. P. Momot1D. A. Orekhov2I. G. Tolstokorov3N. A. Lycheva4V. O. Shevchenko5I. I. Shakhmatov6V. O. Krasyukova7E. V. Fogt8Altai State Medical University (ASMU); Research Institute of Physiology and Fundamental MedicineAltai Branch of National Research Center for Hematology; Research Institute of Physiology and Fundamental MedicineAltai Regional Cardiology Health CenterConsulting Diagnostic Center «Dobryi Doktor» Ltd.Research Institute of Physiology and Fundamental MedicineConsulting Diagnostic Center «Dobryi Doktor» Ltd.Altai State Medical University (ASMU); Research Institute of Physiology and Fundamental MedicineAltai State Medical University (ASMU)Altai State Medical University (ASMU)Aim. The study objective was to examine fibrin monomer hemostatic effects in post-traumatic parenchymal hemorrhage in the setting of pharmacologically associated platelet aggregation inhibition.Materials and methods. In the in vivo experimental study on male rabbits, the hemostatic effects of fibrin monomer (FM) (0.25 mg/kg) were evaluated in comparison with tranexamic acid (TA) (15 mg/kg) in post-traumatic parenchymal hemorrhage against the background of preliminary inhibition of platelet aggregation function with acetylsalicylic acid (2.0 mg/kg) and clopidogrel (8.0 mg/kg). Volume and rate of blood loss were estimated, as well as the parameters of the hemostatic system.Results. It has been established that FM versus placebo when administered intravenously 1 hour before the injury can prevent severe bleeding associated with taking antiplatelet agents. The volume of blood loss after FM administration decreased in median by 6.0 times, the rate of blood loss – by 5.9 times, and when using TA – by 2.4 (РFM-TA < 0.02) and 4.8 times respectively. The hemostatic effects of TA were realized when the hemostatic balance was shifted towards the increased fibrin formation (an increase in the level of D-dimer in the blood plasma). The use of FM was not accompanied by any significant changes in the blood coagulation system.Conclusion. The fibrin monomer at a dose of 0.25 mg/kg i.v. is capable of preventing severe  posttraumatic parenchymal bleeding caused by the combined use of drugs with different antiplatelet action. The phenomenon is not completely clear and needs to be analyzed in further research. The mechanism of hemostatic effects associated with FM is currently being studied.https://bulletin.ssmu.ru/jour/article/view/2678fibrin monomeracetylsalicylic acidclopidogreltranexamic acidhemostatic effect
spellingShingle V. M. Vdovin
A. P. Momot
D. A. Orekhov
I. G. Tolstokorov
N. A. Lycheva
V. O. Shevchenko
I. I. Shakhmatov
V. O. Krasyukova
E. V. Fogt
Experimental study of systemic hemostatic effects of fibrin monomer in inhibition of platelet aggregation function
Бюллетень сибирской медицины
fibrin monomer
acetylsalicylic acid
clopidogrel
tranexamic acid
hemostatic effect
title Experimental study of systemic hemostatic effects of fibrin monomer in inhibition of platelet aggregation function
title_full Experimental study of systemic hemostatic effects of fibrin monomer in inhibition of platelet aggregation function
title_fullStr Experimental study of systemic hemostatic effects of fibrin monomer in inhibition of platelet aggregation function
title_full_unstemmed Experimental study of systemic hemostatic effects of fibrin monomer in inhibition of platelet aggregation function
title_short Experimental study of systemic hemostatic effects of fibrin monomer in inhibition of platelet aggregation function
title_sort experimental study of systemic hemostatic effects of fibrin monomer in inhibition of platelet aggregation function
topic fibrin monomer
acetylsalicylic acid
clopidogrel
tranexamic acid
hemostatic effect
url https://bulletin.ssmu.ru/jour/article/view/2678
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