Comparison of fluorescent intercalating dyes for quantitative loop-mediated isothermal amplification (qLAMP)
Real-time or quantitative loop-mediated isothermal amplification (qLAMP) is a promising technique for the accurate detection of pathogens in organisms and the environment. Here we present a comparative study of the performance of six fluorescent intercalating dyes—SYTO-9, SYTO-13, SYTO-82, SYBR Gree...
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| Main Authors: | , , , , |
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| Format: | Article |
| Language: | English |
| Published: |
Taylor & Francis Group
2016-07-01
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| Series: | BioTechniques |
| Subjects: | |
| Online Access: | https://www.future-science.com/doi/10.2144/000114432 |
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| Summary: | Real-time or quantitative loop-mediated isothermal amplification (qLAMP) is a promising technique for the accurate detection of pathogens in organisms and the environment. Here we present a comparative study of the performance of six fluorescent intercalating dyes—SYTO-9, SYTO-13, SYTO-82, SYBR Green I, SYBR Gold, EvaGreen—in three different qLAMP model systems. SYTO-9 and SYTO-82, which had the best results, were used for additional enzyme and template titration studies. SYTO-82 demonstrated the best combination of time-to-threshold (Tt) and signal-to-noise ratio (SNR). |
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| ISSN: | 0736-6205 1940-9818 |