Real Time Detection and Association of Helicobacter Pylori in Oral Squamous Cell Carcinoma

Background: Helicobacter pylori is a recognized pathogen associated with gastric carcinoma, but its potential role in oral squamous cell carcinoma (OSCC) remains underinvestigated. Objective: To evaluate the prevalence of H. pylori in OSCC patients and compare it with healthy controls, assessing its...

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Main Authors: Kandasamy Murugan, Kesavaram Padmavathy, Philips Mathew, Nalini Ashwath, R C Neil Ananth, T Saravanan
Format: Article
Language:English
Published: Wolters Kluwer Medknow Publications 2025-04-01
Series:Journal of Indian Academy of Oral Medicine and Radiology
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Online Access:https://journals.lww.com/10.4103/jiaomr.jiaomr_25_25
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author Kandasamy Murugan
Kesavaram Padmavathy
Philips Mathew
Nalini Ashwath
R C Neil Ananth
T Saravanan
author_facet Kandasamy Murugan
Kesavaram Padmavathy
Philips Mathew
Nalini Ashwath
R C Neil Ananth
T Saravanan
author_sort Kandasamy Murugan
collection DOAJ
description Background: Helicobacter pylori is a recognized pathogen associated with gastric carcinoma, but its potential role in oral squamous cell carcinoma (OSCC) remains underinvestigated. Objective: To evaluate the prevalence of H. pylori in OSCC patients and compare it with healthy controls, assessing its potential etiological role in OSCC. Methods: This study included 30 participants, OSCC (n = 15) patients and healthy controls (n = 15). Unstimulated saliva was collected from all the study participants, and DNA was extracted, aliquoted, and stored. H. pylori HPUreA gene was detected in the saliva samples by real-time PCR. Histopathological confirmation of the clinical diagnosis of OSCC was performed for the patient group. Statistical analysis was performed to compare the prevalence of H. pylori between the groups and the associations with clinical variables, including lifestyle factors such as smoking and betel nut chewing. Results: Statistically significant difference (P < 0.05) was observed in the prevalence of H. pylori between OSCC patients (73.33%) and the healthy controls (26.67%) who tested positive. Nevertheless, no difference in the mean CT value for the positive samples in the control group Vs OSCC group (36.6 ± 1.26 Vs 37.19 ± 1.31) was not statistically significant (P > 0.05). Conclusion: The findings suggest a strong association between H. pylori and OSCC, highlighting the potential role of the bacterium in oral carcinogenesis and warranting further research to understand the underlying mechanisms.
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issn 0972-1363
0975-1572
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spelling doaj-art-472f0e2fa5ee436aa51e145be2d7e23b2025-08-20T03:28:55ZengWolters Kluwer Medknow PublicationsJournal of Indian Academy of Oral Medicine and Radiology0972-13630975-15722025-04-0137217618010.4103/jiaomr.jiaomr_25_25Real Time Detection and Association of Helicobacter Pylori in Oral Squamous Cell CarcinomaKandasamy MuruganKesavaram PadmavathyPhilips MathewNalini AshwathR C Neil AnanthT SaravananBackground: Helicobacter pylori is a recognized pathogen associated with gastric carcinoma, but its potential role in oral squamous cell carcinoma (OSCC) remains underinvestigated. Objective: To evaluate the prevalence of H. pylori in OSCC patients and compare it with healthy controls, assessing its potential etiological role in OSCC. Methods: This study included 30 participants, OSCC (n = 15) patients and healthy controls (n = 15). Unstimulated saliva was collected from all the study participants, and DNA was extracted, aliquoted, and stored. H. pylori HPUreA gene was detected in the saliva samples by real-time PCR. Histopathological confirmation of the clinical diagnosis of OSCC was performed for the patient group. Statistical analysis was performed to compare the prevalence of H. pylori between the groups and the associations with clinical variables, including lifestyle factors such as smoking and betel nut chewing. Results: Statistically significant difference (P < 0.05) was observed in the prevalence of H. pylori between OSCC patients (73.33%) and the healthy controls (26.67%) who tested positive. Nevertheless, no difference in the mean CT value for the positive samples in the control group Vs OSCC group (36.6 ± 1.26 Vs 37.19 ± 1.31) was not statistically significant (P > 0.05). Conclusion: The findings suggest a strong association between H. pylori and OSCC, highlighting the potential role of the bacterium in oral carcinogenesis and warranting further research to understand the underlying mechanisms.https://journals.lww.com/10.4103/jiaomr.jiaomr_25_25biomarkerstumorhelicobacter pylorioral squamous cell carcinomareal-time polymerase chain reactionsquamous cell carcinoma of head and neckurease
spellingShingle Kandasamy Murugan
Kesavaram Padmavathy
Philips Mathew
Nalini Ashwath
R C Neil Ananth
T Saravanan
Real Time Detection and Association of Helicobacter Pylori in Oral Squamous Cell Carcinoma
Journal of Indian Academy of Oral Medicine and Radiology
biomarkers
tumor
helicobacter pylori
oral squamous cell carcinoma
real-time polymerase chain reaction
squamous cell carcinoma of head and neck
urease
title Real Time Detection and Association of Helicobacter Pylori in Oral Squamous Cell Carcinoma
title_full Real Time Detection and Association of Helicobacter Pylori in Oral Squamous Cell Carcinoma
title_fullStr Real Time Detection and Association of Helicobacter Pylori in Oral Squamous Cell Carcinoma
title_full_unstemmed Real Time Detection and Association of Helicobacter Pylori in Oral Squamous Cell Carcinoma
title_short Real Time Detection and Association of Helicobacter Pylori in Oral Squamous Cell Carcinoma
title_sort real time detection and association of helicobacter pylori in oral squamous cell carcinoma
topic biomarkers
tumor
helicobacter pylori
oral squamous cell carcinoma
real-time polymerase chain reaction
squamous cell carcinoma of head and neck
urease
url https://journals.lww.com/10.4103/jiaomr.jiaomr_25_25
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