Concentration-dependent effect of norfloxacin on iron toxicity in the intestine of large yellow croaker Larimichthys crocea (Richardson, 1846)
This study explores the effects of norfloxacin (NOR) on oxidative damage, iron (Fe) transport, energy metabolism, and immunotoxicity in the intestine of large yellow croaker under Fe stress. The fish were subjected to Fe (180 μg/L), low-dose NOR (1.8 μg/L, LNOR), high-dose NOR (180 μg/L, HNOR), Fe p...
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Frontiers Media S.A.
2025-01-01
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| Series: | Frontiers in Marine Science |
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| Online Access: | https://www.frontiersin.org/articles/10.3389/fmars.2024.1530807/full |
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| author | Lin Zeng Yong-Hong Wang Chun-Xiang Ai Bin Liu Bin Liu Min-Hui Yu Hui Zhang Fei Li |
| author_facet | Lin Zeng Yong-Hong Wang Chun-Xiang Ai Bin Liu Bin Liu Min-Hui Yu Hui Zhang Fei Li |
| author_sort | Lin Zeng |
| collection | DOAJ |
| description | This study explores the effects of norfloxacin (NOR) on oxidative damage, iron (Fe) transport, energy metabolism, and immunotoxicity in the intestine of large yellow croaker under Fe stress. The fish were subjected to Fe (180 μg/L), low-dose NOR (1.8 μg/L, LNOR), high-dose NOR (180 μg/L, HNOR), Fe plus LNOR, and Fe plus HNOR for 60 days. These results demonstrated that Fe alone exposure increased malondialdehyde (MDA), protein carboxylation (PC), and mortality rate, and impaired intestinal tissue, which was related to the increment of Fe accumulation. Compared to Fe alone exposure, Fe plus LNOR exposure decreased MDA, PC, and mortality rate, and alleviated intestinal malformations by improving Fe transport, energy metabolism, anti-inflammatory response, and protein folding protective effect, and reducing pro-inflammatory response, indicating that LNOR had an antagonistic effect on Fe toxicity. Compared to Fe alone exposure, Fe plus HNOR exposure elevated MDA, PC, and mortality rate, and deteriorated intestinal malformations by inhibiting Fe excretion, energy metabolism, anti-inflammatory response, and protein folding protective effect, and enhancing pro-inflammatory response, indicating a synergetic effect between HNOR and Fe stress. These findings suggested that NOR had a dose-dependent effect on Fe-toxicity to large yellow croaker, which contributes to revealing the molecular mechanisms behind their interaction and its ecological implications. |
| format | Article |
| id | doaj-art-47105afc4ca3437da04ac352dbaec508 |
| institution | Kabale University |
| issn | 2296-7745 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Frontiers Media S.A. |
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| series | Frontiers in Marine Science |
| spelling | doaj-art-47105afc4ca3437da04ac352dbaec5082025-01-22T05:19:28ZengFrontiers Media S.A.Frontiers in Marine Science2296-77452025-01-011110.3389/fmars.2024.15308071530807Concentration-dependent effect of norfloxacin on iron toxicity in the intestine of large yellow croaker Larimichthys crocea (Richardson, 1846)Lin Zeng0Yong-Hong Wang1Chun-Xiang Ai2Bin Liu3Bin Liu4Min-Hui Yu5Hui Zhang6Fei Li7College of Food and Biological Engineering, Bengbu University, Bengbu, ChinaCollege of Food and Biological Engineering, Bengbu University, Bengbu, ChinaCollege of Ocean and Earth Sciences, Xiamen University, Xiamen, ChinaState Key Laboratory of Mariculture Biobreeding and Sustainable Goods, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, ChinaLaboratory for Marine Fisheries Science and Food Production Processes, Qingdao Marine Science and Technology Center, Qingdao, ChinaCollege of Ocean and Earth Sciences, Xiamen University, Xiamen, ChinaNational Engineering Research Center of Marine Facilities Aquaculture, Zhejiang Ocean University, Zhoushan, ChinaCollege of Fishery Economics, Tangshan Maritime Institute, Tangshan, ChinaThis study explores the effects of norfloxacin (NOR) on oxidative damage, iron (Fe) transport, energy metabolism, and immunotoxicity in the intestine of large yellow croaker under Fe stress. The fish were subjected to Fe (180 μg/L), low-dose NOR (1.8 μg/L, LNOR), high-dose NOR (180 μg/L, HNOR), Fe plus LNOR, and Fe plus HNOR for 60 days. These results demonstrated that Fe alone exposure increased malondialdehyde (MDA), protein carboxylation (PC), and mortality rate, and impaired intestinal tissue, which was related to the increment of Fe accumulation. Compared to Fe alone exposure, Fe plus LNOR exposure decreased MDA, PC, and mortality rate, and alleviated intestinal malformations by improving Fe transport, energy metabolism, anti-inflammatory response, and protein folding protective effect, and reducing pro-inflammatory response, indicating that LNOR had an antagonistic effect on Fe toxicity. Compared to Fe alone exposure, Fe plus HNOR exposure elevated MDA, PC, and mortality rate, and deteriorated intestinal malformations by inhibiting Fe excretion, energy metabolism, anti-inflammatory response, and protein folding protective effect, and enhancing pro-inflammatory response, indicating a synergetic effect between HNOR and Fe stress. These findings suggested that NOR had a dose-dependent effect on Fe-toxicity to large yellow croaker, which contributes to revealing the molecular mechanisms behind their interaction and its ecological implications.https://www.frontiersin.org/articles/10.3389/fmars.2024.1530807/fulliron exposurenorfloxacinNF-κBHSPLarimichthys crocea |
| spellingShingle | Lin Zeng Yong-Hong Wang Chun-Xiang Ai Bin Liu Bin Liu Min-Hui Yu Hui Zhang Fei Li Concentration-dependent effect of norfloxacin on iron toxicity in the intestine of large yellow croaker Larimichthys crocea (Richardson, 1846) Frontiers in Marine Science iron exposure norfloxacin NF-κB HSP Larimichthys crocea |
| title | Concentration-dependent effect of norfloxacin on iron toxicity in the intestine of large yellow croaker Larimichthys crocea (Richardson, 1846) |
| title_full | Concentration-dependent effect of norfloxacin on iron toxicity in the intestine of large yellow croaker Larimichthys crocea (Richardson, 1846) |
| title_fullStr | Concentration-dependent effect of norfloxacin on iron toxicity in the intestine of large yellow croaker Larimichthys crocea (Richardson, 1846) |
| title_full_unstemmed | Concentration-dependent effect of norfloxacin on iron toxicity in the intestine of large yellow croaker Larimichthys crocea (Richardson, 1846) |
| title_short | Concentration-dependent effect of norfloxacin on iron toxicity in the intestine of large yellow croaker Larimichthys crocea (Richardson, 1846) |
| title_sort | concentration dependent effect of norfloxacin on iron toxicity in the intestine of large yellow croaker larimichthys crocea richardson 1846 |
| topic | iron exposure norfloxacin NF-κB HSP Larimichthys crocea |
| url | https://www.frontiersin.org/articles/10.3389/fmars.2024.1530807/full |
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