Transcriptomic analysis identifies key genes associated with nutrient deprivation responses in infectious bursal disease virus infection
Abstract Infectious bursal disease virus (IBDV) causes a highly contagious and immunosuppressive disease that severely impacts the poultry industry. However, the molecular basis of IBDV-induced immunosuppression remains poorly understood. In this study, we investigate key genes implicated in this pr...
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| Format: | Article |
| Language: | English |
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BMC
2025-08-01
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| Series: | BMC Genomics |
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| Online Access: | https://doi.org/10.1186/s12864-025-11918-x |
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| author | Jiaxing Wang Qiangzhou Wang Yuyu Ping Xuan Huang Ting Yang Zongping Liu Hao Bai Guobin Chang Shihao Chen |
| author_facet | Jiaxing Wang Qiangzhou Wang Yuyu Ping Xuan Huang Ting Yang Zongping Liu Hao Bai Guobin Chang Shihao Chen |
| author_sort | Jiaxing Wang |
| collection | DOAJ |
| description | Abstract Infectious bursal disease virus (IBDV) causes a highly contagious and immunosuppressive disease that severely impacts the poultry industry. However, the molecular basis of IBDV-induced immunosuppression remains poorly understood. In this study, we investigate key genes implicated in this process, focusing on the interplay between metabolism and immune regulation. IBDV infection enhanced glucose and glutamine uptake in HD11 cells. Transcriptome analysis across glucose-starved, amino acid-starved, IBDV-infected, and control groups identified key differentially expressed genes (DEGs) linked to immune and metabolic pathways. Specifically, 612 DEGs were associated with glucose starvation, 998 with amino acid starvation, and 496 shared between both conditions. Enrichment analysis revealed involvement in cytokine signalling, amino acid biosynthesis, and antiviral responses. qPCR validated ENO2 and MAT1A expression changes, and a regulatory network analysis highlighted their potential roles in nutrient stress and IBDV pathogenesis. This study presents a novel strategy to identify genes involved in nutrient deprivation during IBDV infection, highlighting ENO2 and MAT1A as key contributors to immune cell dysfunction. |
| format | Article |
| id | doaj-art-471005c2bd0b43c0a77cb00db28b419b |
| institution | Kabale University |
| issn | 1471-2164 |
| language | English |
| publishDate | 2025-08-01 |
| publisher | BMC |
| record_format | Article |
| series | BMC Genomics |
| spelling | doaj-art-471005c2bd0b43c0a77cb00db28b419b2025-08-20T03:43:57ZengBMCBMC Genomics1471-21642025-08-0126111210.1186/s12864-025-11918-xTranscriptomic analysis identifies key genes associated with nutrient deprivation responses in infectious bursal disease virus infectionJiaxing Wang0Qiangzhou Wang1Yuyu Ping2Xuan Huang3Ting Yang4Zongping Liu5Hao Bai6Guobin Chang7Shihao Chen8College of Animal Science and Technology, Yangzhou UniversityCollege of Animal Science and Technology, Yangzhou UniversityCollege of Veterinary Medicine, Yangzhou UniversityCollege of Animal Science and Technology, Yangzhou UniversityCollege of Animal Science and Technology, Yangzhou UniversityCollege of Veterinary Medicine, Yangzhou UniversityCollege of Animal Science and Technology, Yangzhou UniversityCollege of Animal Science and Technology, Yangzhou UniversityCollege of Animal Science and Technology, Yangzhou UniversityAbstract Infectious bursal disease virus (IBDV) causes a highly contagious and immunosuppressive disease that severely impacts the poultry industry. However, the molecular basis of IBDV-induced immunosuppression remains poorly understood. In this study, we investigate key genes implicated in this process, focusing on the interplay between metabolism and immune regulation. IBDV infection enhanced glucose and glutamine uptake in HD11 cells. Transcriptome analysis across glucose-starved, amino acid-starved, IBDV-infected, and control groups identified key differentially expressed genes (DEGs) linked to immune and metabolic pathways. Specifically, 612 DEGs were associated with glucose starvation, 998 with amino acid starvation, and 496 shared between both conditions. Enrichment analysis revealed involvement in cytokine signalling, amino acid biosynthesis, and antiviral responses. qPCR validated ENO2 and MAT1A expression changes, and a regulatory network analysis highlighted their potential roles in nutrient stress and IBDV pathogenesis. This study presents a novel strategy to identify genes involved in nutrient deprivation during IBDV infection, highlighting ENO2 and MAT1A as key contributors to immune cell dysfunction.https://doi.org/10.1186/s12864-025-11918-xTranscriptomicsInfectious bursal disease virusNutrient deprivation responseMacrophages |
| spellingShingle | Jiaxing Wang Qiangzhou Wang Yuyu Ping Xuan Huang Ting Yang Zongping Liu Hao Bai Guobin Chang Shihao Chen Transcriptomic analysis identifies key genes associated with nutrient deprivation responses in infectious bursal disease virus infection BMC Genomics Transcriptomics Infectious bursal disease virus Nutrient deprivation response Macrophages |
| title | Transcriptomic analysis identifies key genes associated with nutrient deprivation responses in infectious bursal disease virus infection |
| title_full | Transcriptomic analysis identifies key genes associated with nutrient deprivation responses in infectious bursal disease virus infection |
| title_fullStr | Transcriptomic analysis identifies key genes associated with nutrient deprivation responses in infectious bursal disease virus infection |
| title_full_unstemmed | Transcriptomic analysis identifies key genes associated with nutrient deprivation responses in infectious bursal disease virus infection |
| title_short | Transcriptomic analysis identifies key genes associated with nutrient deprivation responses in infectious bursal disease virus infection |
| title_sort | transcriptomic analysis identifies key genes associated with nutrient deprivation responses in infectious bursal disease virus infection |
| topic | Transcriptomics Infectious bursal disease virus Nutrient deprivation response Macrophages |
| url | https://doi.org/10.1186/s12864-025-11918-x |
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