Expression and prognostic value of estrogen-related receptor β alternative splicing isoforms in esophageal squamous cell carcinoma
Abstract Background Estrogen-related receptor β (ERRβ) alternative splicing isoforms, including ERRβsf, ERRβ2, and ERRβΔ10, have been implicated in the pathogenesis of malignant tumors. Nevertheless, their specific impact on esophageal squamous cell carcinoma (ESCC) remains unclear. The study aimed...
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BMC
2025-05-01
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| Series: | European Journal of Medical Research |
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| Online Access: | https://doi.org/10.1186/s40001-025-02638-9 |
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| author | Kai-Yuan Jiang Jia-Xin Wan Hong-Yun Li Wei-Yang Chen Chun-Mei Shen Ke-Xuan Guo Xiang-Yun Zheng Hong-Ying Wen Dong Tian |
| author_facet | Kai-Yuan Jiang Jia-Xin Wan Hong-Yun Li Wei-Yang Chen Chun-Mei Shen Ke-Xuan Guo Xiang-Yun Zheng Hong-Ying Wen Dong Tian |
| author_sort | Kai-Yuan Jiang |
| collection | DOAJ |
| description | Abstract Background Estrogen-related receptor β (ERRβ) alternative splicing isoforms, including ERRβsf, ERRβ2, and ERRβΔ10, have been implicated in the pathogenesis of malignant tumors. Nevertheless, their specific impact on esophageal squamous cell carcinoma (ESCC) remains unclear. The study aimed to investigate the expression and prognostic value of ERRβ alternative splicing isoforms in ESCC. Methods This study prospectively collected ESCC tissues and paired normal tissues of 54 patients with ESCC who underwent esophagectomy without neoadjuvant therapy. The protein expression levels of ERRβ alternative splicing isoforms in ESCC and normal tissues were detected by Western blot. The Kaplan–Meier method with a log-rank test was used to estimate overall survival (OS). The Cox proportional hazards regression analysis was used to evaluate the independent prognostic factors. Results In ESCC tissues, the ERRβsf/ERRβ ratio was significantly higher (P = 0.017) compared to paired normal tissues. Based on a cut-off value of 0.24, there were 18 and 36 cases in the high ERRβsf/ERRβ expression group and low ERRβsf/ERRβ expression group, respectively. Patients with high ERRβsf/ERRβ ratios had significantly better OS than those patients with low ERRβsf/ERRβ ratios (77.1% vs 50.8%, P = 0.024). The multivariate analysis revealed that ERRβsf/ERRβ (hazard ratio [HR] = 0.219, 95% confidence interval [CI] 0.063–0.767, P = 0.018) and N stage (HR = 7.892, 95% CI 1.328–46.911, P = 0.023) were independent prognostic factors for ESCC patients. Conclusions This study is the first to demonstrate the relationship between ERRβ alternative splicing isoforms in ESCC. A high ERRβsf/ERRβ ratio was associated with a better prognosis, indicating that ERRβ alternative splicing isoforms may serve as potential prognostic biomarkers for ESCC. |
| format | Article |
| id | doaj-art-470f83e2bdfb4804bac81d5ea3e92b66 |
| institution | OA Journals |
| issn | 2047-783X |
| language | English |
| publishDate | 2025-05-01 |
| publisher | BMC |
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| series | European Journal of Medical Research |
| spelling | doaj-art-470f83e2bdfb4804bac81d5ea3e92b662025-08-20T02:15:00ZengBMCEuropean Journal of Medical Research2047-783X2025-05-013011810.1186/s40001-025-02638-9Expression and prognostic value of estrogen-related receptor β alternative splicing isoforms in esophageal squamous cell carcinomaKai-Yuan Jiang0Jia-Xin Wan1Hong-Yun Li2Wei-Yang Chen3Chun-Mei Shen4Ke-Xuan Guo5Xiang-Yun Zheng6Hong-Ying Wen7Dong Tian8Department of Cardiothoracic Intensive Care Unit, Affiliated Hospital of North Sichuan Medical CollegeDepartment of Thoracic Surgery, West China Hospital, Sichuan UniversityDepartment of Cardiothoracic Intensive Care Unit, Affiliated Hospital of North Sichuan Medical CollegeDepartment of Thoracic Surgery, The First Hospital of China Medical UniversityDepartment of Cardiothoracic Intensive Care Unit, Affiliated Hospital of North Sichuan Medical CollegeDepartment of Cardiothoracic Intensive Care Unit, Affiliated Hospital of North Sichuan Medical CollegeDepartment of Thoracic Surgery, West China Hospital, Sichuan UniversityDepartment of Cardiothoracic Intensive Care Unit, Affiliated Hospital of North Sichuan Medical CollegeDepartment of Thoracic Surgery, West China Hospital, Sichuan UniversityAbstract Background Estrogen-related receptor β (ERRβ) alternative splicing isoforms, including ERRβsf, ERRβ2, and ERRβΔ10, have been implicated in the pathogenesis of malignant tumors. Nevertheless, their specific impact on esophageal squamous cell carcinoma (ESCC) remains unclear. The study aimed to investigate the expression and prognostic value of ERRβ alternative splicing isoforms in ESCC. Methods This study prospectively collected ESCC tissues and paired normal tissues of 54 patients with ESCC who underwent esophagectomy without neoadjuvant therapy. The protein expression levels of ERRβ alternative splicing isoforms in ESCC and normal tissues were detected by Western blot. The Kaplan–Meier method with a log-rank test was used to estimate overall survival (OS). The Cox proportional hazards regression analysis was used to evaluate the independent prognostic factors. Results In ESCC tissues, the ERRβsf/ERRβ ratio was significantly higher (P = 0.017) compared to paired normal tissues. Based on a cut-off value of 0.24, there were 18 and 36 cases in the high ERRβsf/ERRβ expression group and low ERRβsf/ERRβ expression group, respectively. Patients with high ERRβsf/ERRβ ratios had significantly better OS than those patients with low ERRβsf/ERRβ ratios (77.1% vs 50.8%, P = 0.024). The multivariate analysis revealed that ERRβsf/ERRβ (hazard ratio [HR] = 0.219, 95% confidence interval [CI] 0.063–0.767, P = 0.018) and N stage (HR = 7.892, 95% CI 1.328–46.911, P = 0.023) were independent prognostic factors for ESCC patients. Conclusions This study is the first to demonstrate the relationship between ERRβ alternative splicing isoforms in ESCC. A high ERRβsf/ERRβ ratio was associated with a better prognosis, indicating that ERRβ alternative splicing isoforms may serve as potential prognostic biomarkers for ESCC.https://doi.org/10.1186/s40001-025-02638-9Esophageal squamous cell carcinomaEstrogen-related receptor βAlternative splicingPrognosis |
| spellingShingle | Kai-Yuan Jiang Jia-Xin Wan Hong-Yun Li Wei-Yang Chen Chun-Mei Shen Ke-Xuan Guo Xiang-Yun Zheng Hong-Ying Wen Dong Tian Expression and prognostic value of estrogen-related receptor β alternative splicing isoforms in esophageal squamous cell carcinoma European Journal of Medical Research Esophageal squamous cell carcinoma Estrogen-related receptor β Alternative splicing Prognosis |
| title | Expression and prognostic value of estrogen-related receptor β alternative splicing isoforms in esophageal squamous cell carcinoma |
| title_full | Expression and prognostic value of estrogen-related receptor β alternative splicing isoforms in esophageal squamous cell carcinoma |
| title_fullStr | Expression and prognostic value of estrogen-related receptor β alternative splicing isoforms in esophageal squamous cell carcinoma |
| title_full_unstemmed | Expression and prognostic value of estrogen-related receptor β alternative splicing isoforms in esophageal squamous cell carcinoma |
| title_short | Expression and prognostic value of estrogen-related receptor β alternative splicing isoforms in esophageal squamous cell carcinoma |
| title_sort | expression and prognostic value of estrogen related receptor β alternative splicing isoforms in esophageal squamous cell carcinoma |
| topic | Esophageal squamous cell carcinoma Estrogen-related receptor β Alternative splicing Prognosis |
| url | https://doi.org/10.1186/s40001-025-02638-9 |
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