Cyclocarya paliurus extract activates insulin signaling via Sirtuin1 in C2C12 myotubes and decreases blood glucose level in mice with impaired insulin secretion.
Diabetes is caused by the lack of release or action of insulin. Some foods and supplements can compensate for this deficiency; thus, they can aid in the prevention or treatment of diabetes. The aim of this study was to investigate the effects of Cyclocarya paliurus extract (CPE) on insulin signaling...
Saved in:
| Main Authors: | , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Public Library of Science (PLoS)
2017-01-01
|
| Series: | PLoS ONE |
| Online Access: | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0183988&type=printable |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849715563788500992 |
|---|---|
| author | Hisae Yoshitomi Rika Tsuru Linyi Li Jingxin Zhou Maya Kudo Tonghua Liu Ming Gao |
| author_facet | Hisae Yoshitomi Rika Tsuru Linyi Li Jingxin Zhou Maya Kudo Tonghua Liu Ming Gao |
| author_sort | Hisae Yoshitomi |
| collection | DOAJ |
| description | Diabetes is caused by the lack of release or action of insulin. Some foods and supplements can compensate for this deficiency; thus, they can aid in the prevention or treatment of diabetes. The aim of this study was to investigate the effects of Cyclocarya paliurus extract (CPE) on insulin signaling and its capacity to correct hyperglycemia in the absence of insulin. To investigate the hypoglycemic effects of CPE, C2C12 cells were exposed to CPE (50 and 100 μg/mL). CPE promoted 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2NBDG) uptake into the cells via translocation of glucose transporter 4 (Glut4) to the plasma membrane. In addition, CPE enhanced tyrosine phosphorylation of insulin receptor substrate and activated phosphatidylinositol 3-kinase and protein kinase B (Akt) via sirtuin1 in C2C12 cells. Moreover, we found that oral administration of CPE (1 g/kg) to streptozotocin-induced hyperglycemic mice produced a progressive decrease in plasma glucose levels at 1 h after single dosing. At that point, CPE significantly increased the expression of skeletal muscle membrane Glut4 and enhanced the phosphorylation of Akt. These results suggest that CPE exerts antidiabetic effects similar to those of insulin, and may be an oral therapeutic alternative for the management of diabetes. |
| format | Article |
| id | doaj-art-470b77b728b04270acfbb72e4b8e96e2 |
| institution | DOAJ |
| issn | 1932-6203 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Public Library of Science (PLoS) |
| record_format | Article |
| series | PLoS ONE |
| spelling | doaj-art-470b77b728b04270acfbb72e4b8e96e22025-08-20T03:13:19ZengPublic Library of Science (PLoS)PLoS ONE1932-62032017-01-01128e018398810.1371/journal.pone.0183988Cyclocarya paliurus extract activates insulin signaling via Sirtuin1 in C2C12 myotubes and decreases blood glucose level in mice with impaired insulin secretion.Hisae YoshitomiRika TsuruLinyi LiJingxin ZhouMaya KudoTonghua LiuMing GaoDiabetes is caused by the lack of release or action of insulin. Some foods and supplements can compensate for this deficiency; thus, they can aid in the prevention or treatment of diabetes. The aim of this study was to investigate the effects of Cyclocarya paliurus extract (CPE) on insulin signaling and its capacity to correct hyperglycemia in the absence of insulin. To investigate the hypoglycemic effects of CPE, C2C12 cells were exposed to CPE (50 and 100 μg/mL). CPE promoted 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2NBDG) uptake into the cells via translocation of glucose transporter 4 (Glut4) to the plasma membrane. In addition, CPE enhanced tyrosine phosphorylation of insulin receptor substrate and activated phosphatidylinositol 3-kinase and protein kinase B (Akt) via sirtuin1 in C2C12 cells. Moreover, we found that oral administration of CPE (1 g/kg) to streptozotocin-induced hyperglycemic mice produced a progressive decrease in plasma glucose levels at 1 h after single dosing. At that point, CPE significantly increased the expression of skeletal muscle membrane Glut4 and enhanced the phosphorylation of Akt. These results suggest that CPE exerts antidiabetic effects similar to those of insulin, and may be an oral therapeutic alternative for the management of diabetes.https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0183988&type=printable |
| spellingShingle | Hisae Yoshitomi Rika Tsuru Linyi Li Jingxin Zhou Maya Kudo Tonghua Liu Ming Gao Cyclocarya paliurus extract activates insulin signaling via Sirtuin1 in C2C12 myotubes and decreases blood glucose level in mice with impaired insulin secretion. PLoS ONE |
| title | Cyclocarya paliurus extract activates insulin signaling via Sirtuin1 in C2C12 myotubes and decreases blood glucose level in mice with impaired insulin secretion. |
| title_full | Cyclocarya paliurus extract activates insulin signaling via Sirtuin1 in C2C12 myotubes and decreases blood glucose level in mice with impaired insulin secretion. |
| title_fullStr | Cyclocarya paliurus extract activates insulin signaling via Sirtuin1 in C2C12 myotubes and decreases blood glucose level in mice with impaired insulin secretion. |
| title_full_unstemmed | Cyclocarya paliurus extract activates insulin signaling via Sirtuin1 in C2C12 myotubes and decreases blood glucose level in mice with impaired insulin secretion. |
| title_short | Cyclocarya paliurus extract activates insulin signaling via Sirtuin1 in C2C12 myotubes and decreases blood glucose level in mice with impaired insulin secretion. |
| title_sort | cyclocarya paliurus extract activates insulin signaling via sirtuin1 in c2c12 myotubes and decreases blood glucose level in mice with impaired insulin secretion |
| url | https://journals.plos.org/plosone/article/file?id=10.1371/journal.pone.0183988&type=printable |
| work_keys_str_mv | AT hisaeyoshitomi cyclocaryapaliurusextractactivatesinsulinsignalingviasirtuin1inc2c12myotubesanddecreasesbloodglucoselevelinmicewithimpairedinsulinsecretion AT rikatsuru cyclocaryapaliurusextractactivatesinsulinsignalingviasirtuin1inc2c12myotubesanddecreasesbloodglucoselevelinmicewithimpairedinsulinsecretion AT linyili cyclocaryapaliurusextractactivatesinsulinsignalingviasirtuin1inc2c12myotubesanddecreasesbloodglucoselevelinmicewithimpairedinsulinsecretion AT jingxinzhou cyclocaryapaliurusextractactivatesinsulinsignalingviasirtuin1inc2c12myotubesanddecreasesbloodglucoselevelinmicewithimpairedinsulinsecretion AT mayakudo cyclocaryapaliurusextractactivatesinsulinsignalingviasirtuin1inc2c12myotubesanddecreasesbloodglucoselevelinmicewithimpairedinsulinsecretion AT tonghualiu cyclocaryapaliurusextractactivatesinsulinsignalingviasirtuin1inc2c12myotubesanddecreasesbloodglucoselevelinmicewithimpairedinsulinsecretion AT minggao cyclocaryapaliurusextractactivatesinsulinsignalingviasirtuin1inc2c12myotubesanddecreasesbloodglucoselevelinmicewithimpairedinsulinsecretion |