The Effect of Glutathione on The Quality of Aceh Local Catfish (Clarias gariepinus) Spermatozoa After Cryopreservation
The cryopreservation process might reduce the quality of spermatozoa due to an increase in the production of reactive oxygen species (ROS) compounds during cooling, freezing, and thawing. The quality of spermatozoa can be maintained by adding glutathione as an exogenous antioxidant into cryo-diluent...
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Universitas Negeri Semarang
2020-04-01
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| Series: | Biosaintifika: Journal of Biology & Biology Education |
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| Online Access: | https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/22353 |
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| author | Raudhah Mahfudhah Kartini Eriani Zainal Abidin Muchlisin Cut Ruhul Muthmainnah |
| author_facet | Raudhah Mahfudhah Kartini Eriani Zainal Abidin Muchlisin Cut Ruhul Muthmainnah |
| author_sort | Raudhah Mahfudhah |
| collection | DOAJ |
| description | The cryopreservation process might reduce the quality of spermatozoa due to an increase in the production of reactive oxygen species (ROS) compounds during cooling, freezing, and thawing. The quality of spermatozoa can be maintained by adding glutathione as an exogenous antioxidant into cryo-diluent. This study aimed to examine the effect of the addition of different doses of glutathione in cryopreservation of Aceh Local catfish (Clarias gariepinus) spermatozoa after freezing. The method used was a completely randomized design (CRD) with four treatments and four replications. Fresh semen was diluted in Ringer, 15% DMSO, and 20% Fetal Bovine Serum (FBS) and then added with glutathione antioxidants of 0.0 mgL-1, 0.5 mgL-1, 1.0 mgL-1, and 2.0 mgL-1. The parameters observed in this study were motility, integrity of the plasma membrane, fertility, and DNA integrity. The results showed that the concentration of glutathione had no effect on motility, integrity ofthe plasma membrane, or fertility, but had an effect on DNA integrity. The highest percentage of motility and plasma membrane integrity respectively was 40.50% (P3) and 70.87% (P2). Furthermore, the assessment of DNA integrity showed that there was no DNA fragmentation both treatments and fresh spermatozoa. This research is the first study regarding glutathione supplementation in cryo-diluent of Aceh Local catfish spermatozoa. Finally, the results obtained can provide information about the exact concentration of glutathione in the extender on the quality of spermatozoa of Aceh Local catfish (C. gariepinus) after the cryopreservation process. These results can also increase the success of fertility be used by the seed hall unit and the aquaculture industry to increase the productivity and supply high quality seeds. |
| format | Article |
| id | doaj-art-47040792bcb24d45bc3f9faf82d3241b |
| institution | DOAJ |
| issn | 2085-191X 2338-7610 |
| language | English |
| publishDate | 2020-04-01 |
| publisher | Universitas Negeri Semarang |
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| series | Biosaintifika: Journal of Biology & Biology Education |
| spelling | doaj-art-47040792bcb24d45bc3f9faf82d3241b2025-08-20T02:39:19ZengUniversitas Negeri SemarangBiosaintifika: Journal of Biology & Biology Education2085-191X2338-76102020-04-0112112513110.15294/biosaintifika.v12i1.223539661The Effect of Glutathione on The Quality of Aceh Local Catfish (Clarias gariepinus) Spermatozoa After CryopreservationRaudhah Mahfudhah0Kartini Eriani1Zainal Abidin Muchlisin2Cut Ruhul Muthmainnah3Master Program of Biology, Faculty of Mathematics and Natural Sciences, Universitas Syiah KualaDepartment of Biology, Faculty of Mathematics and Natural Sciences, Universitas Syiah KualaDepartment of Aquaculture, Faculty of Marine and Fisheries, Universitas Syiah KualaMaster Program of Biology, Faculty of Mathematics and Natural Sciences, Universitas Syiah Kuala, IndonesiaThe cryopreservation process might reduce the quality of spermatozoa due to an increase in the production of reactive oxygen species (ROS) compounds during cooling, freezing, and thawing. The quality of spermatozoa can be maintained by adding glutathione as an exogenous antioxidant into cryo-diluent. This study aimed to examine the effect of the addition of different doses of glutathione in cryopreservation of Aceh Local catfish (Clarias gariepinus) spermatozoa after freezing. The method used was a completely randomized design (CRD) with four treatments and four replications. Fresh semen was diluted in Ringer, 15% DMSO, and 20% Fetal Bovine Serum (FBS) and then added with glutathione antioxidants of 0.0 mgL-1, 0.5 mgL-1, 1.0 mgL-1, and 2.0 mgL-1. The parameters observed in this study were motility, integrity of the plasma membrane, fertility, and DNA integrity. The results showed that the concentration of glutathione had no effect on motility, integrity ofthe plasma membrane, or fertility, but had an effect on DNA integrity. The highest percentage of motility and plasma membrane integrity respectively was 40.50% (P3) and 70.87% (P2). Furthermore, the assessment of DNA integrity showed that there was no DNA fragmentation both treatments and fresh spermatozoa. This research is the first study regarding glutathione supplementation in cryo-diluent of Aceh Local catfish spermatozoa. Finally, the results obtained can provide information about the exact concentration of glutathione in the extender on the quality of spermatozoa of Aceh Local catfish (C. gariepinus) after the cryopreservation process. These results can also increase the success of fertility be used by the seed hall unit and the aquaculture industry to increase the productivity and supply high quality seeds.https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/22353antioxidantsglutathionecryopreservationspermatozoa |
| spellingShingle | Raudhah Mahfudhah Kartini Eriani Zainal Abidin Muchlisin Cut Ruhul Muthmainnah The Effect of Glutathione on The Quality of Aceh Local Catfish (Clarias gariepinus) Spermatozoa After Cryopreservation Biosaintifika: Journal of Biology & Biology Education antioxidants glutathione cryopreservation spermatozoa |
| title | The Effect of Glutathione on The Quality of Aceh Local Catfish (Clarias gariepinus) Spermatozoa After Cryopreservation |
| title_full | The Effect of Glutathione on The Quality of Aceh Local Catfish (Clarias gariepinus) Spermatozoa After Cryopreservation |
| title_fullStr | The Effect of Glutathione on The Quality of Aceh Local Catfish (Clarias gariepinus) Spermatozoa After Cryopreservation |
| title_full_unstemmed | The Effect of Glutathione on The Quality of Aceh Local Catfish (Clarias gariepinus) Spermatozoa After Cryopreservation |
| title_short | The Effect of Glutathione on The Quality of Aceh Local Catfish (Clarias gariepinus) Spermatozoa After Cryopreservation |
| title_sort | effect of glutathione on the quality of aceh local catfish clarias gariepinus spermatozoa after cryopreservation |
| topic | antioxidants glutathione cryopreservation spermatozoa |
| url | https://journal.unnes.ac.id/nju/index.php/biosaintifika/article/view/22353 |
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