Estrogen-mediated TRPV5 modulates proliferation and apoptosis of rat cochlear hair cells via the PI3K/Akt pathway
Objective: Transient Receptor Potential Vanilloid 5 (TRPV5) is an essential Ca2+ transporter mediated by estrogen in osteoclast differentiation and bone resorption. However, the mechanism of how estrogen mediates TRPV5 expression in Benign Paroxysmal Positional Vertigo (BPPV) remains unclear. Method...
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| Format: | Article |
| Language: | English |
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Elsevier
2025-11-01
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| Series: | Brazilian Journal of Otorhinolaryngology |
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| Online Access: | http://www.sciencedirect.com/science/article/pii/S1808869425001430 |
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| _version_ | 1849229299772555264 |
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| author | Yao Zhou Shouju Huang Lijuan Zhao Chengzhen Pan Jianguo Wang Shuxia Qian |
| author_facet | Yao Zhou Shouju Huang Lijuan Zhao Chengzhen Pan Jianguo Wang Shuxia Qian |
| author_sort | Yao Zhou |
| collection | DOAJ |
| description | Objective: Transient Receptor Potential Vanilloid 5 (TRPV5) is an essential Ca2+ transporter mediated by estrogen in osteoclast differentiation and bone resorption. However, the mechanism of how estrogen mediates TRPV5 expression in Benign Paroxysmal Positional Vertigo (BPPV) remains unclear. Methods: In this study, the Ovariectomized (OVX) rats were established to explore whether TRPV5 was regulated by estrogen. Results: Lower protein expression level of TRPV5 showed in OVX rats were found to be restored by injection of Estradiol (E2). The same result was observed when TRPV5 expression was disturbed by si-TRPV5 fragment transfection. In addition, overexpression of TRPV5 up-regulated the expression of pho-Akt (Ser473) and promoted proliferation and apoptosis of cochlear hair cells. Conclusion: Estrogen may contribute to BPPV by stimulating TRPV5 overexpression to further up-regulate the expression of pho-Akt (Ser473) and promote cell proliferation and apoptosis. Our study provides preliminary insights into the pathogenesis of BPPV. |
| format | Article |
| id | doaj-art-46f540d608d84ad1b321a5de8d03ed21 |
| institution | Kabale University |
| issn | 1808-8694 |
| language | English |
| publishDate | 2025-11-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Brazilian Journal of Otorhinolaryngology |
| spelling | doaj-art-46f540d608d84ad1b321a5de8d03ed212025-08-22T04:55:59ZengElsevierBrazilian Journal of Otorhinolaryngology1808-86942025-11-0191610169910.1016/j.bjorl.2025.101699Estrogen-mediated TRPV5 modulates proliferation and apoptosis of rat cochlear hair cells via the PI3K/Akt pathwayYao Zhou0Shouju Huang1Lijuan Zhao2Chengzhen Pan3Jianguo Wang4Shuxia Qian5Zhejiang Chinese Medical University, Zhejiang, China; Department of Neurology, the Second Affiliated Hospital of Jiaxing University, Zhejiang, ChinaDepartment of Neurology, Lu'an Hospital of Anhui Medical University, Anhui, ChinaZhejiang Chinese Medical University, Zhejiang, ChinaDepartment of Neurology, the Second Affiliated Hospital of Guizhou Medical University, Guizhou, ChinaCentral Laboratory, Jiaxing Maternity and Child Health Care Hospital, Zhejiang, China; Corresponding authors.Department of Neurology, the Second Affiliated Hospital of Jiaxing University, Zhejiang, China; Corresponding authors.Objective: Transient Receptor Potential Vanilloid 5 (TRPV5) is an essential Ca2+ transporter mediated by estrogen in osteoclast differentiation and bone resorption. However, the mechanism of how estrogen mediates TRPV5 expression in Benign Paroxysmal Positional Vertigo (BPPV) remains unclear. Methods: In this study, the Ovariectomized (OVX) rats were established to explore whether TRPV5 was regulated by estrogen. Results: Lower protein expression level of TRPV5 showed in OVX rats were found to be restored by injection of Estradiol (E2). The same result was observed when TRPV5 expression was disturbed by si-TRPV5 fragment transfection. In addition, overexpression of TRPV5 up-regulated the expression of pho-Akt (Ser473) and promoted proliferation and apoptosis of cochlear hair cells. Conclusion: Estrogen may contribute to BPPV by stimulating TRPV5 overexpression to further up-regulate the expression of pho-Akt (Ser473) and promote cell proliferation and apoptosis. Our study provides preliminary insights into the pathogenesis of BPPV.http://www.sciencedirect.com/science/article/pii/S1808869425001430BPPVEstrogenE2TRPV5Akt |
| spellingShingle | Yao Zhou Shouju Huang Lijuan Zhao Chengzhen Pan Jianguo Wang Shuxia Qian Estrogen-mediated TRPV5 modulates proliferation and apoptosis of rat cochlear hair cells via the PI3K/Akt pathway Brazilian Journal of Otorhinolaryngology BPPV Estrogen E2 TRPV5 Akt |
| title | Estrogen-mediated TRPV5 modulates proliferation and apoptosis of rat cochlear hair cells via the PI3K/Akt pathway |
| title_full | Estrogen-mediated TRPV5 modulates proliferation and apoptosis of rat cochlear hair cells via the PI3K/Akt pathway |
| title_fullStr | Estrogen-mediated TRPV5 modulates proliferation and apoptosis of rat cochlear hair cells via the PI3K/Akt pathway |
| title_full_unstemmed | Estrogen-mediated TRPV5 modulates proliferation and apoptosis of rat cochlear hair cells via the PI3K/Akt pathway |
| title_short | Estrogen-mediated TRPV5 modulates proliferation and apoptosis of rat cochlear hair cells via the PI3K/Akt pathway |
| title_sort | estrogen mediated trpv5 modulates proliferation and apoptosis of rat cochlear hair cells via the pi3k akt pathway |
| topic | BPPV Estrogen E2 TRPV5 Akt |
| url | http://www.sciencedirect.com/science/article/pii/S1808869425001430 |
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