The Effect of MSCs Derived from the Human Umbilical Cord Transduced by Fibroblast Growth Factor-20 on Parkinson’s Disease
Cell therapy is a potential therapeutic approach for Parkinson’s disease (PD). Mesenchymal stem cells derived from the human umbilical cord (hUC-MSCs) give priority to PD patients because of multiple advantages. The appropriate gene transduction of hUC-MSC before transplantation is a promising proce...
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| Main Authors: | , , , , , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2016-01-01
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| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2016/5016768 |
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| author | Li Jinfeng Wang Yunliang Liu Xinshan Wang Shanshan Xu Chunyang Xue Peng Yang Xiaopeng Xu Zhixiu Yin Honglei Cao Xia Duan Haifeng Cao Bingzhen |
| author_facet | Li Jinfeng Wang Yunliang Liu Xinshan Wang Shanshan Xu Chunyang Xue Peng Yang Xiaopeng Xu Zhixiu Yin Honglei Cao Xia Duan Haifeng Cao Bingzhen |
| author_sort | Li Jinfeng |
| collection | DOAJ |
| description | Cell therapy is a potential therapeutic approach for Parkinson’s disease (PD). Mesenchymal stem cells derived from the human umbilical cord (hUC-MSCs) give priority to PD patients because of multiple advantages. The appropriate gene transduction of hUC-MSC before transplantation is a promising procedure for cell therapy. Fibroblast growth factor-20 (FGF-20) has been shown to protect dopaminergic neurons against a range of toxic insults in vitro. In this study, the hUC-MSCs were gene transduced with FGF-20, and then we transplanted them into the PD mice model. The results showed that MSC-FGF-20 treatment obviously improved the behavior of PD, accompanied by the increase of tyrosine carboxylase- (TH-) positive cell and dopamine (DA). Furtherly, immunohistochemistry disclosed that MSC-FGF-20 obviously promoted the degradation of nuclear factor-κB (NF-κB), a transcription factor that controls genes encoding proinflammatory cytokines, highly expressed in the nigrostriatal dopaminergic regions in PD patients. Therefore, MSC-FGF-20 has a potential for improving PD, closely related to the degradation of NF-κB. |
| format | Article |
| id | doaj-art-46f4710e00c14b4c8d05fb76b523e6d7 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2016-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-46f4710e00c14b4c8d05fb76b523e6d72025-08-20T03:36:45ZengWileyStem Cells International1687-966X1687-96782016-01-01201610.1155/2016/50167685016768The Effect of MSCs Derived from the Human Umbilical Cord Transduced by Fibroblast Growth Factor-20 on Parkinson’s DiseaseLi Jinfeng0Wang Yunliang1Liu Xinshan2Wang Shanshan3Xu Chunyang4Xue Peng5Yang Xiaopeng6Xu Zhixiu7Yin Honglei8Cao Xia9Duan Haifeng10Cao Bingzhen11Neurology Department, General Hospital of Jinan Military Region, Jinan, Shandong 250031, ChinaNeurology Department, The Second Hospital Affiliated to Zhengzhou University, Zhengzhou, Henan 450014, ChinaSanbo Brain Hospital, Capital Medical University, Haidian District, Beijing 100093, ChinaNeurology Department, The 148th Hospital, Zibo, Shandong 255300, ChinaNeurology Department, The Second Hospital Affiliated to Zhengzhou University, Zhengzhou, Henan 450014, ChinaNeurology Department, The Second Hospital Affiliated to Zhengzhou University, Zhengzhou, Henan 450014, ChinaNeurology Department, The Second Hospital Affiliated to Zhengzhou University, Zhengzhou, Henan 450014, ChinaNeurology Department, The Second Hospital Affiliated to Zhengzhou University, Zhengzhou, Henan 450014, ChinaNeurology Department, The 148th Hospital, Zibo, Shandong 255300, ChinaNeurology Department, General Hospital of Jinan Military Region, Jinan, Shandong 250031, ChinaDepartment of Experimental Hematology, Beijing Institute of Radiation Medicine, 27 Taiping Road, Beijing 100850, ChinaNeurology Department, General Hospital of Jinan Military Region, Jinan, Shandong 250031, ChinaCell therapy is a potential therapeutic approach for Parkinson’s disease (PD). Mesenchymal stem cells derived from the human umbilical cord (hUC-MSCs) give priority to PD patients because of multiple advantages. The appropriate gene transduction of hUC-MSC before transplantation is a promising procedure for cell therapy. Fibroblast growth factor-20 (FGF-20) has been shown to protect dopaminergic neurons against a range of toxic insults in vitro. In this study, the hUC-MSCs were gene transduced with FGF-20, and then we transplanted them into the PD mice model. The results showed that MSC-FGF-20 treatment obviously improved the behavior of PD, accompanied by the increase of tyrosine carboxylase- (TH-) positive cell and dopamine (DA). Furtherly, immunohistochemistry disclosed that MSC-FGF-20 obviously promoted the degradation of nuclear factor-κB (NF-κB), a transcription factor that controls genes encoding proinflammatory cytokines, highly expressed in the nigrostriatal dopaminergic regions in PD patients. Therefore, MSC-FGF-20 has a potential for improving PD, closely related to the degradation of NF-κB.http://dx.doi.org/10.1155/2016/5016768 |
| spellingShingle | Li Jinfeng Wang Yunliang Liu Xinshan Wang Shanshan Xu Chunyang Xue Peng Yang Xiaopeng Xu Zhixiu Yin Honglei Cao Xia Duan Haifeng Cao Bingzhen The Effect of MSCs Derived from the Human Umbilical Cord Transduced by Fibroblast Growth Factor-20 on Parkinson’s Disease Stem Cells International |
| title | The Effect of MSCs Derived from the Human Umbilical Cord Transduced by Fibroblast Growth Factor-20 on Parkinson’s Disease |
| title_full | The Effect of MSCs Derived from the Human Umbilical Cord Transduced by Fibroblast Growth Factor-20 on Parkinson’s Disease |
| title_fullStr | The Effect of MSCs Derived from the Human Umbilical Cord Transduced by Fibroblast Growth Factor-20 on Parkinson’s Disease |
| title_full_unstemmed | The Effect of MSCs Derived from the Human Umbilical Cord Transduced by Fibroblast Growth Factor-20 on Parkinson’s Disease |
| title_short | The Effect of MSCs Derived from the Human Umbilical Cord Transduced by Fibroblast Growth Factor-20 on Parkinson’s Disease |
| title_sort | effect of mscs derived from the human umbilical cord transduced by fibroblast growth factor 20 on parkinson s disease |
| url | http://dx.doi.org/10.1155/2016/5016768 |
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