Nucleic Acid, Antibody, and Virus Culture Methods to Detect Xenotropic MLV-Related Virus in Human Blood Samples
The MLV-related retrovirus, XMRV, was recently identified and reported to be associated with both prostate cancer and chronic fatigue syndrome. At the National Cancer Institute-Frederick, MD (NCI-Frederick), we developed highly sensitive methods to detect XMRV nucleic acids, antibodies, and replicat...
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| Format: | Article |
| Language: | English |
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Wiley
2011-01-01
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| Series: | Advances in Virology |
| Online Access: | http://dx.doi.org/10.1155/2011/272193 |
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| author | M. F. Kearney K. Lee R. K. Bagni A. Wiegand J. Spindler F. Maldarelli P. A. Pinto W. M. Linehan C. D. Vocke K. A. Delviks-Frankenberry R. W. deVere White G. Q. Del Prete J. W. Mellors J. D. Lifson V. N. KewalRamani V. K. Pathak J. M. Coffin S. F. J. Le Grice |
| author_facet | M. F. Kearney K. Lee R. K. Bagni A. Wiegand J. Spindler F. Maldarelli P. A. Pinto W. M. Linehan C. D. Vocke K. A. Delviks-Frankenberry R. W. deVere White G. Q. Del Prete J. W. Mellors J. D. Lifson V. N. KewalRamani V. K. Pathak J. M. Coffin S. F. J. Le Grice |
| author_sort | M. F. Kearney |
| collection | DOAJ |
| description | The MLV-related retrovirus, XMRV, was recently identified and reported to be associated with both prostate cancer and chronic fatigue syndrome. At the National Cancer Institute-Frederick, MD (NCI-Frederick), we developed highly sensitive methods to detect XMRV nucleic acids, antibodies, and replication competent virus. Analysis of XMRV-spiked samples and/or specimens from two pigtail macaques experimentally inoculated with 22Rv1 cell-derived XMRV confirmed the ability of the assays used to detect XMRV RNA and DNA, and culture isolatable virus when present, along with XMRV reactive antibody responses. Using these assays, we did not detect evidence of XMRV in blood samples () or prostate specimens () from two independent cohorts of patients with prostate cancer. Previous studies detected XMRV in prostate tissues. In the present study, we primarily investigated the levels of XMRV in blood plasma samples collected from patients with prostate cancer. These results demonstrate that while XMRV-related assays developed at the NCI-Frederick can readily measure XMRV nucleic acids, antibodies, and replication competent virus, no evidence of XMRV was found in the blood of patients with prostate cancer. |
| format | Article |
| id | doaj-art-46eedb94a7d148eb9da1e544bb67561d |
| institution | Kabale University |
| issn | 1687-8639 1687-8647 |
| language | English |
| publishDate | 2011-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Virology |
| spelling | doaj-art-46eedb94a7d148eb9da1e544bb67561d2025-02-03T01:26:41ZengWileyAdvances in Virology1687-86391687-86472011-01-01201110.1155/2011/272193272193Nucleic Acid, Antibody, and Virus Culture Methods to Detect Xenotropic MLV-Related Virus in Human Blood SamplesM. F. Kearney0K. Lee1R. K. Bagni2A. Wiegand3J. Spindler4F. Maldarelli5P. A. Pinto6W. M. Linehan7C. D. Vocke8K. A. Delviks-Frankenberry9R. W. deVere White10G. Q. Del Prete11J. W. Mellors12J. D. Lifson13V. N. KewalRamani14V. K. Pathak15J. M. Coffin16S. F. J. Le Grice17HIV Drug Resistance Program, National Cancer Institute at Frederick, Frederick, MD 21702-1201, USAHIV Drug Resistance Program, National Cancer Institute at Frederick, Frederick, MD 21702-1201, USAProtein Expression Laboratory, SAIC-Frederick, Inc., NCI-Frederick, Frederick, MD 21702, USAHIV Drug Resistance Program, National Cancer Institute at Frederick, Frederick, MD 21702-1201, USAHIV Drug Resistance Program, National Cancer Institute at Frederick, Frederick, MD 21702-1201, USAHIV Drug Resistance Program, National Cancer Institute at Frederick, Frederick, MD 21702-1201, USAUrologic Oncology Branch, National Cancer Institute, Bethesda, MD 20892, USAUrologic Oncology Branch, National Cancer Institute, Bethesda, MD 20892, USAUrologic Oncology Branch, National Cancer Institute, Bethesda, MD 20892, USAHIV Drug Resistance Program, National Cancer Institute at Frederick, Frederick, MD 21702-1201, USAUC Davis Cancer Center, Sacramento, CA 95817, USAAIDS and Cancer Virus Program, SAIC-Frederick, Inc., National Cancer Institute, Frederick, MD 21702, USADepartment of Medicine, University of Pittsburgh, Pittsburgh, PA 15260, USAAIDS and Cancer Virus Program, SAIC-Frederick, Inc., National Cancer Institute, Frederick, MD 21702, USAHIV Drug Resistance Program, National Cancer Institute at Frederick, Frederick, MD 21702-1201, USAHIV Drug Resistance Program, National Cancer Institute at Frederick, Frederick, MD 21702-1201, USADepartment of Molecular Biology and Microbiology, Tufts University, Boston, MA 02155, USAHIV Drug Resistance Program, National Cancer Institute at Frederick, Frederick, MD 21702-1201, USAThe MLV-related retrovirus, XMRV, was recently identified and reported to be associated with both prostate cancer and chronic fatigue syndrome. At the National Cancer Institute-Frederick, MD (NCI-Frederick), we developed highly sensitive methods to detect XMRV nucleic acids, antibodies, and replication competent virus. Analysis of XMRV-spiked samples and/or specimens from two pigtail macaques experimentally inoculated with 22Rv1 cell-derived XMRV confirmed the ability of the assays used to detect XMRV RNA and DNA, and culture isolatable virus when present, along with XMRV reactive antibody responses. Using these assays, we did not detect evidence of XMRV in blood samples () or prostate specimens () from two independent cohorts of patients with prostate cancer. Previous studies detected XMRV in prostate tissues. In the present study, we primarily investigated the levels of XMRV in blood plasma samples collected from patients with prostate cancer. These results demonstrate that while XMRV-related assays developed at the NCI-Frederick can readily measure XMRV nucleic acids, antibodies, and replication competent virus, no evidence of XMRV was found in the blood of patients with prostate cancer.http://dx.doi.org/10.1155/2011/272193 |
| spellingShingle | M. F. Kearney K. Lee R. K. Bagni A. Wiegand J. Spindler F. Maldarelli P. A. Pinto W. M. Linehan C. D. Vocke K. A. Delviks-Frankenberry R. W. deVere White G. Q. Del Prete J. W. Mellors J. D. Lifson V. N. KewalRamani V. K. Pathak J. M. Coffin S. F. J. Le Grice Nucleic Acid, Antibody, and Virus Culture Methods to Detect Xenotropic MLV-Related Virus in Human Blood Samples Advances in Virology |
| title | Nucleic Acid, Antibody, and Virus Culture Methods to Detect Xenotropic MLV-Related Virus in Human Blood Samples |
| title_full | Nucleic Acid, Antibody, and Virus Culture Methods to Detect Xenotropic MLV-Related Virus in Human Blood Samples |
| title_fullStr | Nucleic Acid, Antibody, and Virus Culture Methods to Detect Xenotropic MLV-Related Virus in Human Blood Samples |
| title_full_unstemmed | Nucleic Acid, Antibody, and Virus Culture Methods to Detect Xenotropic MLV-Related Virus in Human Blood Samples |
| title_short | Nucleic Acid, Antibody, and Virus Culture Methods to Detect Xenotropic MLV-Related Virus in Human Blood Samples |
| title_sort | nucleic acid antibody and virus culture methods to detect xenotropic mlv related virus in human blood samples |
| url | http://dx.doi.org/10.1155/2011/272193 |
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