The effect of five versus two personnel on bacterial air contamination during preparation of sterile surgical goods in the operating room: a randomised controlled trial
Abstract Background Surgical site infection (SSI) and antimicrobial resistance are a worldwide problem affecting patient safety. It is lacking randomised controlled trials (RCT) regarding how the number of personnel in the operating room (OR) affects the air quality. We aimed to investigate the effe...
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| Main Authors: | , , |
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| Format: | Article |
| Language: | English |
| Published: |
BMC
2025-06-01
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| Series: | Antimicrobial Resistance and Infection Control |
| Subjects: | |
| Online Access: | https://doi.org/10.1186/s13756-025-01589-4 |
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| Summary: | Abstract Background Surgical site infection (SSI) and antimicrobial resistance are a worldwide problem affecting patient safety. It is lacking randomised controlled trials (RCT) regarding how the number of personnel in the operating room (OR) affects the air quality. We aimed to investigate the effect the number of personnel in the OR have on bacterial air contamination during the preparation of sterile surgical goods, to identify the species and antibiotic susceptibility of the bacteria isolated, and to describe the number of SSIs together with causative microorganisms. Methods This RCT used an intervention group in which two individuals prepared the surgical goods and a control group in which five individuals prepared the goods. Bacteria were isolated on aerobic and anaerobic plates, and bacterial growth was measured as colony forming units (CFU). All isolates were typed, and types known to cause SSI were tested for susceptibility to eight antibiotics. Data were analysed with the Mann-Whitney U test, the chi-square test, or Fisher’s exact test. Results Results were based on 69 open-heart surgeries and 414 plates. When sterile surgical goods were prepared with two personnel, the median CFU was 2 with an IQR of 2, compared with five personnel, the median CFU was 5, with an IQR of 5 (p < 0.001). The 272 CFU represented 45 different bacterial species, with 38 species isolated in the control group and 21 in the intervention group. The most frequently isolated bacteria were Cutibacterium acnes (82/272, 30%), and Staphylococcus epidermidis (36/272, 13%). Of the 36 S. epidermidis isolates, 11 (31%) were drug-resistant, including three multidrug-resistant. One patient in the control group was infected by Staphyloccocus aureus and Staphylococcus lugdunensis, neither of which was isolated during the preparation of sterile goods. One patient in the intervention group developed an SSI caused by C. acnes, Corynebacterium kroppenstedtii, and S. epidermidis. C. acnes and S. epidermidis were isolated during the preparation. Conclusions Minimising the number of personnel in the OR during preparation of sterile surgical goods is important to reduce the bacterial load. Trial registration Prospectively 15 May 2022 at FoU Sweden (275659) and retrospectively 22 October 2022 at ClinicalTrials.Gov (NCT05597072). |
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| ISSN: | 2047-2994 |