Ionic liquid ultrasound-assisted extraction (IL-UAE) for duck feather keratin and in silico evaluation as a potential procollagen n-endopeptidase inhibitor
This research aims to optimize keratin extraction from duck feathers using an eco-friendly ionic liquid-ultrasound-assisted extraction (IL-UAE) method and evaluate its potential applications in tissue engineering. It investigated the effects of deposition pH (1-6), ultrasonication temperature (40-60...
Saved in:
| Main Authors: | , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Komunitas Ilmuwan dan Profesional Muslim Indonesia
2025-07-01
|
| Series: | Communications in Science and Technology |
| Subjects: | |
| Online Access: | https://cst.kipmi.or.id/journal/article/view/1669 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850036767225282560 |
|---|---|
| author | Maria Monica Sianita Nita Kusumawati Mahanani Tri Asri Shod Abdurrachman Dzulkarnain Achmad Naufal Al Hafidl Ashabul Kahfi Mutiara Azfa Nabila Ferdiansyah Setiawan Luthfiyah Isyrak Khofifatul Rahmawati |
| author_facet | Maria Monica Sianita Nita Kusumawati Mahanani Tri Asri Shod Abdurrachman Dzulkarnain Achmad Naufal Al Hafidl Ashabul Kahfi Mutiara Azfa Nabila Ferdiansyah Setiawan Luthfiyah Isyrak Khofifatul Rahmawati |
| author_sort | Maria Monica Sianita |
| collection | DOAJ |
| description | This research aims to optimize keratin extraction from duck feathers using an eco-friendly ionic liquid-ultrasound-assisted extraction (IL-UAE) method and evaluate its potential applications in tissue engineering. It investigated the effects of deposition pH (1-6), ultrasonication temperature (40-60°C), and time (60-180 min) on extraction yield and physicochemical properties. The results demonstrated the optimal extraction conditions at pH 3, 40°C, and 60 minutes, yielding 82% keratin with a 0.50 mg/mL concentration, while the lowest yield production was found at pH 6 (33%, 0.20 mg/mL). Meanwhile, characterization via FTIR confirmed predominant ?-sheet structures with characteristic peaks at 3250-3300 cm?¹ (N-H/O-H stretching) and 1700-1500 cm?¹ (C=O stretching). SDS-PAGE revealed pure keratin bands (10-15 kDa), while SEM showed layered, porous morphology suitable for biomaterial applications. Thermogravimetric analysis, furthermore, identified three degradation stages occurred at 0-200°C (3.05% loss), 200-400°C (39.37% loss), and 400-700°C (31.13% loss). Amino acid profiling revealed high L-cystine content (153,064.90-156,926.33 mg/kg) with the significant amounts of glycine (63,958.25-64,064.73 mg/kg), L-proline (77,631.16-77,717.42 mg/kg), and L-leucine (59,111.43-59,198.60 mg/kg). In silico molecular docking studies identified leucine as a promising procollagen N-endopeptidase inhibitor (binding energy -5.0 kcal/mol), which controlled the collagen-breaking and forming process. This ability makes keratin potential to be developed as a scaffold for bone tissue regeneration in medical industry. |
| format | Article |
| id | doaj-art-46e59c207e5e414f8747e2dae170fbbd |
| institution | DOAJ |
| issn | 2502-9258 2502-9266 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Komunitas Ilmuwan dan Profesional Muslim Indonesia |
| record_format | Article |
| series | Communications in Science and Technology |
| spelling | doaj-art-46e59c207e5e414f8747e2dae170fbbd2025-08-20T02:57:04ZengKomunitas Ilmuwan dan Profesional Muslim IndonesiaCommunications in Science and Technology2502-92582502-92662025-07-01101758610.21924/cst.10.1.2025.16691669Ionic liquid ultrasound-assisted extraction (IL-UAE) for duck feather keratin and in silico evaluation as a potential procollagen n-endopeptidase inhibitorMaria Monica SianitaNita Kusumawati0Mahanani Tri Asri1Shod Abdurrachman Dzulkarnain2Achmad Naufal Al Hafidl3Ashabul Kahfi4Mutiara Azfa Nabila5Ferdiansyah Setiawan6Luthfiyah Isyrak7Khofifatul Rahmawati8Universitas Negeri SurabayaUniversitas Negeri SurabayaUniversitas Negeri SurabayaUniversitas Negeri SurabayaUniversitas Negeri SurabayaUniversitas Negeri SurabayaUniversitas Negeri SurabayaUniversitas Negeri SurabayaUniversitas Negeri SurabayaThis research aims to optimize keratin extraction from duck feathers using an eco-friendly ionic liquid-ultrasound-assisted extraction (IL-UAE) method and evaluate its potential applications in tissue engineering. It investigated the effects of deposition pH (1-6), ultrasonication temperature (40-60°C), and time (60-180 min) on extraction yield and physicochemical properties. The results demonstrated the optimal extraction conditions at pH 3, 40°C, and 60 minutes, yielding 82% keratin with a 0.50 mg/mL concentration, while the lowest yield production was found at pH 6 (33%, 0.20 mg/mL). Meanwhile, characterization via FTIR confirmed predominant ?-sheet structures with characteristic peaks at 3250-3300 cm?¹ (N-H/O-H stretching) and 1700-1500 cm?¹ (C=O stretching). SDS-PAGE revealed pure keratin bands (10-15 kDa), while SEM showed layered, porous morphology suitable for biomaterial applications. Thermogravimetric analysis, furthermore, identified three degradation stages occurred at 0-200°C (3.05% loss), 200-400°C (39.37% loss), and 400-700°C (31.13% loss). Amino acid profiling revealed high L-cystine content (153,064.90-156,926.33 mg/kg) with the significant amounts of glycine (63,958.25-64,064.73 mg/kg), L-proline (77,631.16-77,717.42 mg/kg), and L-leucine (59,111.43-59,198.60 mg/kg). In silico molecular docking studies identified leucine as a promising procollagen N-endopeptidase inhibitor (binding energy -5.0 kcal/mol), which controlled the collagen-breaking and forming process. This ability makes keratin potential to be developed as a scaffold for bone tissue regeneration in medical industry.https://cst.kipmi.or.id/journal/article/view/1669keratinduck feathersionic liquidultrasound-assisted extractionprocollagen n-endopeptidase inhibitor |
| spellingShingle | Maria Monica Sianita Nita Kusumawati Mahanani Tri Asri Shod Abdurrachman Dzulkarnain Achmad Naufal Al Hafidl Ashabul Kahfi Mutiara Azfa Nabila Ferdiansyah Setiawan Luthfiyah Isyrak Khofifatul Rahmawati Ionic liquid ultrasound-assisted extraction (IL-UAE) for duck feather keratin and in silico evaluation as a potential procollagen n-endopeptidase inhibitor Communications in Science and Technology keratin duck feathers ionic liquid ultrasound-assisted extraction procollagen n-endopeptidase inhibitor |
| title | Ionic liquid ultrasound-assisted extraction (IL-UAE) for duck feather keratin and in silico evaluation as a potential procollagen n-endopeptidase inhibitor |
| title_full | Ionic liquid ultrasound-assisted extraction (IL-UAE) for duck feather keratin and in silico evaluation as a potential procollagen n-endopeptidase inhibitor |
| title_fullStr | Ionic liquid ultrasound-assisted extraction (IL-UAE) for duck feather keratin and in silico evaluation as a potential procollagen n-endopeptidase inhibitor |
| title_full_unstemmed | Ionic liquid ultrasound-assisted extraction (IL-UAE) for duck feather keratin and in silico evaluation as a potential procollagen n-endopeptidase inhibitor |
| title_short | Ionic liquid ultrasound-assisted extraction (IL-UAE) for duck feather keratin and in silico evaluation as a potential procollagen n-endopeptidase inhibitor |
| title_sort | ionic liquid ultrasound assisted extraction il uae for duck feather keratin and in silico evaluation as a potential procollagen n endopeptidase inhibitor |
| topic | keratin duck feathers ionic liquid ultrasound-assisted extraction procollagen n-endopeptidase inhibitor |
| url | https://cst.kipmi.or.id/journal/article/view/1669 |
| work_keys_str_mv | AT mariamonicasianita ionicliquidultrasoundassistedextractioniluaeforduckfeatherkeratinandinsilicoevaluationasapotentialprocollagennendopeptidaseinhibitor AT nitakusumawati ionicliquidultrasoundassistedextractioniluaeforduckfeatherkeratinandinsilicoevaluationasapotentialprocollagennendopeptidaseinhibitor AT mahananitriasri ionicliquidultrasoundassistedextractioniluaeforduckfeatherkeratinandinsilicoevaluationasapotentialprocollagennendopeptidaseinhibitor AT shodabdurrachmandzulkarnain ionicliquidultrasoundassistedextractioniluaeforduckfeatherkeratinandinsilicoevaluationasapotentialprocollagennendopeptidaseinhibitor AT achmadnaufalalhafidl ionicliquidultrasoundassistedextractioniluaeforduckfeatherkeratinandinsilicoevaluationasapotentialprocollagennendopeptidaseinhibitor AT ashabulkahfi ionicliquidultrasoundassistedextractioniluaeforduckfeatherkeratinandinsilicoevaluationasapotentialprocollagennendopeptidaseinhibitor AT mutiaraazfanabila ionicliquidultrasoundassistedextractioniluaeforduckfeatherkeratinandinsilicoevaluationasapotentialprocollagennendopeptidaseinhibitor AT ferdiansyahsetiawan ionicliquidultrasoundassistedextractioniluaeforduckfeatherkeratinandinsilicoevaluationasapotentialprocollagennendopeptidaseinhibitor AT luthfiyahisyrak ionicliquidultrasoundassistedextractioniluaeforduckfeatherkeratinandinsilicoevaluationasapotentialprocollagennendopeptidaseinhibitor AT khofifatulrahmawati ionicliquidultrasoundassistedextractioniluaeforduckfeatherkeratinandinsilicoevaluationasapotentialprocollagennendopeptidaseinhibitor |