Advances in the Development and Application of Human Organoids: Techniques, Applications, and Future Perspectives
Organoids are three-dimensional (3D) cell cultures derived from human pluripotent stem cells or adult stem cells that recapitulate the cellular heterogeneity, structure, and function of human organs. These microstructures are invaluable for biomedical research due to their ability to closely mimic t...
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| Format: | Article |
| Language: | English |
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SAGE Publishing
2025-01-01
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| Series: | Cell Transplantation |
| Online Access: | https://doi.org/10.1177/09636897241303271 |
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| author | Zhangcheng Zhu Yiwen Cheng Xia Liu Wenwen Ding Jiaming Liu Zongxin Ling Lingbin Wu |
| author_facet | Zhangcheng Zhu Yiwen Cheng Xia Liu Wenwen Ding Jiaming Liu Zongxin Ling Lingbin Wu |
| author_sort | Zhangcheng Zhu |
| collection | DOAJ |
| description | Organoids are three-dimensional (3D) cell cultures derived from human pluripotent stem cells or adult stem cells that recapitulate the cellular heterogeneity, structure, and function of human organs. These microstructures are invaluable for biomedical research due to their ability to closely mimic the complexity of native tissues while retaining human genetic material. This fidelity to native organ systems positions organoids as a powerful tool for advancing our understanding of human biology and for enhancing preclinical drug testing. Recent advancements have led to the successful development of a variety of organoid types, reflecting a broad range of human organs and tissues. This progress has expanded their application across several domains, including regenerative medicine, where organoids offer potential for tissue replacement and repair; disease modeling, which allows for the study of disease mechanisms and progression in a controlled environment; drug discovery and evaluation, where organoids provide a more accurate platform for testing drug efficacy and safety; and microecological research, where they contribute to understanding the interactions between microbes and host tissues. This review provides a comprehensive overview of the historical development of organoid technology, highlights the key achievements and ongoing challenges in the field, and discusses the current and emerging applications of organoids in both laboratory research and clinical practice. |
| format | Article |
| id | doaj-art-46e59483c18d4d8091f8a5318df7a33d |
| institution | Kabale University |
| issn | 1555-3892 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | SAGE Publishing |
| record_format | Article |
| series | Cell Transplantation |
| spelling | doaj-art-46e59483c18d4d8091f8a5318df7a33d2025-01-28T22:03:46ZengSAGE PublishingCell Transplantation1555-38922025-01-013410.1177/09636897241303271Advances in the Development and Application of Human Organoids: Techniques, Applications, and Future PerspectivesZhangcheng Zhu0Yiwen Cheng1Xia Liu2Wenwen Ding3Jiaming Liu4Zongxin Ling5Lingbin Wu6Department of Preventive Medicine, School of Public Health and Management, Wenzhou Medical University, Wenzhou, ChinaCollaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, ChinaDepartment of Intensive Care Unit, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, ChinaDepartment of Anesthesiology, Affiliated Hospital of Nantong University, Nantong, ChinaDepartment of Preventive Medicine, School of Public Health and Management, Wenzhou Medical University, Wenzhou, ChinaCollaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases, State Key Laboratory for Diagnosis and Treatment of Infectious Diseases, National Clinical Research Center for Infectious Diseases, The First Affiliated Hospital, School of Medicine, Zhejiang University, Hangzhou, ChinaDepartment of Laboratory Medicine, Lishui Second People’s Hospital, Lishui, ChinaOrganoids are three-dimensional (3D) cell cultures derived from human pluripotent stem cells or adult stem cells that recapitulate the cellular heterogeneity, structure, and function of human organs. These microstructures are invaluable for biomedical research due to their ability to closely mimic the complexity of native tissues while retaining human genetic material. This fidelity to native organ systems positions organoids as a powerful tool for advancing our understanding of human biology and for enhancing preclinical drug testing. Recent advancements have led to the successful development of a variety of organoid types, reflecting a broad range of human organs and tissues. This progress has expanded their application across several domains, including regenerative medicine, where organoids offer potential for tissue replacement and repair; disease modeling, which allows for the study of disease mechanisms and progression in a controlled environment; drug discovery and evaluation, where organoids provide a more accurate platform for testing drug efficacy and safety; and microecological research, where they contribute to understanding the interactions between microbes and host tissues. This review provides a comprehensive overview of the historical development of organoid technology, highlights the key achievements and ongoing challenges in the field, and discusses the current and emerging applications of organoids in both laboratory research and clinical practice.https://doi.org/10.1177/09636897241303271 |
| spellingShingle | Zhangcheng Zhu Yiwen Cheng Xia Liu Wenwen Ding Jiaming Liu Zongxin Ling Lingbin Wu Advances in the Development and Application of Human Organoids: Techniques, Applications, and Future Perspectives Cell Transplantation |
| title | Advances in the Development and Application of Human Organoids: Techniques, Applications, and Future Perspectives |
| title_full | Advances in the Development and Application of Human Organoids: Techniques, Applications, and Future Perspectives |
| title_fullStr | Advances in the Development and Application of Human Organoids: Techniques, Applications, and Future Perspectives |
| title_full_unstemmed | Advances in the Development and Application of Human Organoids: Techniques, Applications, and Future Perspectives |
| title_short | Advances in the Development and Application of Human Organoids: Techniques, Applications, and Future Perspectives |
| title_sort | advances in the development and application of human organoids techniques applications and future perspectives |
| url | https://doi.org/10.1177/09636897241303271 |
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