Development of a Colloidal Gold Immunochromatographic Strip for the One-Step Evaluation of the Total Content of Rhein and Aloe-Emodin in Rhubarb

In this study, a colloidal gold immunochromatographic strip was developed for simultaneous detection of rhein and aloe-emodin in rhubarb. The cutoff value, defined as the lowest concentration for which the test line was invisible on the strip, was 50 ng mL−1 for both rhein and aloe-emodin. By contra...

Full description

Saved in:
Bibliographic Details
Main Authors: Ping Sun, Xin-Peng Li, Jie Xin, Tao Xue, Bo Zhang, Yan-Juan Liu
Format: Article
Language:English
Published: Wiley 2022-01-01
Series:International Journal of Analytical Chemistry
Online Access:http://dx.doi.org/10.1155/2022/7067245
Tags: Add Tag
No Tags, Be the first to tag this record!
_version_ 1832554831695314944
author Ping Sun
Xin-Peng Li
Jie Xin
Tao Xue
Bo Zhang
Yan-Juan Liu
author_facet Ping Sun
Xin-Peng Li
Jie Xin
Tao Xue
Bo Zhang
Yan-Juan Liu
author_sort Ping Sun
collection DOAJ
description In this study, a colloidal gold immunochromatographic strip was developed for simultaneous detection of rhein and aloe-emodin in rhubarb. The cutoff value, defined as the lowest concentration for which the test line was invisible on the strip, was 50 ng mL−1 for both rhein and aloe-emodin. By contrast, the cutoff value for emodin was 2,000 ng mL−1. No competitive inhibition was observed up to 5,000 ng mL−1 of physcion, chrysophanol, sennoside A, sennoside B, or rhaponticin. Semiquantitative analyses of the total contents of rhein and aloe-emodin in raw drug materials via our colloidal gold immunochromatographic strips produced results agreeable with those determined by HPLC. Taken together, our findings suggest that the implementation of our colloidal gold immunochromatographic strips provides a rapid one-step method for estimating the total contents of rhein and aloe-emodin, which may represent a powerful tool for quality control of rhubarb.
format Article
id doaj-art-46df652f2cb142dca9354060e2e06472
institution Kabale University
issn 1687-8779
language English
publishDate 2022-01-01
publisher Wiley
record_format Article
series International Journal of Analytical Chemistry
spelling doaj-art-46df652f2cb142dca9354060e2e064722025-02-03T05:50:18ZengWileyInternational Journal of Analytical Chemistry1687-87792022-01-01202210.1155/2022/7067245Development of a Colloidal Gold Immunochromatographic Strip for the One-Step Evaluation of the Total Content of Rhein and Aloe-Emodin in RhubarbPing Sun0Xin-Peng Li1Jie Xin2Tao Xue3Bo Zhang4Yan-Juan Liu5School of PharmacySchool of PharmacySchool of PharmacySchool of PharmacySchool of PharmacySchool of PharmacyIn this study, a colloidal gold immunochromatographic strip was developed for simultaneous detection of rhein and aloe-emodin in rhubarb. The cutoff value, defined as the lowest concentration for which the test line was invisible on the strip, was 50 ng mL−1 for both rhein and aloe-emodin. By contrast, the cutoff value for emodin was 2,000 ng mL−1. No competitive inhibition was observed up to 5,000 ng mL−1 of physcion, chrysophanol, sennoside A, sennoside B, or rhaponticin. Semiquantitative analyses of the total contents of rhein and aloe-emodin in raw drug materials via our colloidal gold immunochromatographic strips produced results agreeable with those determined by HPLC. Taken together, our findings suggest that the implementation of our colloidal gold immunochromatographic strips provides a rapid one-step method for estimating the total contents of rhein and aloe-emodin, which may represent a powerful tool for quality control of rhubarb.http://dx.doi.org/10.1155/2022/7067245
spellingShingle Ping Sun
Xin-Peng Li
Jie Xin
Tao Xue
Bo Zhang
Yan-Juan Liu
Development of a Colloidal Gold Immunochromatographic Strip for the One-Step Evaluation of the Total Content of Rhein and Aloe-Emodin in Rhubarb
International Journal of Analytical Chemistry
title Development of a Colloidal Gold Immunochromatographic Strip for the One-Step Evaluation of the Total Content of Rhein and Aloe-Emodin in Rhubarb
title_full Development of a Colloidal Gold Immunochromatographic Strip for the One-Step Evaluation of the Total Content of Rhein and Aloe-Emodin in Rhubarb
title_fullStr Development of a Colloidal Gold Immunochromatographic Strip for the One-Step Evaluation of the Total Content of Rhein and Aloe-Emodin in Rhubarb
title_full_unstemmed Development of a Colloidal Gold Immunochromatographic Strip for the One-Step Evaluation of the Total Content of Rhein and Aloe-Emodin in Rhubarb
title_short Development of a Colloidal Gold Immunochromatographic Strip for the One-Step Evaluation of the Total Content of Rhein and Aloe-Emodin in Rhubarb
title_sort development of a colloidal gold immunochromatographic strip for the one step evaluation of the total content of rhein and aloe emodin in rhubarb
url http://dx.doi.org/10.1155/2022/7067245
work_keys_str_mv AT pingsun developmentofacolloidalgoldimmunochromatographicstripfortheonestepevaluationofthetotalcontentofrheinandaloeemodininrhubarb
AT xinpengli developmentofacolloidalgoldimmunochromatographicstripfortheonestepevaluationofthetotalcontentofrheinandaloeemodininrhubarb
AT jiexin developmentofacolloidalgoldimmunochromatographicstripfortheonestepevaluationofthetotalcontentofrheinandaloeemodininrhubarb
AT taoxue developmentofacolloidalgoldimmunochromatographicstripfortheonestepevaluationofthetotalcontentofrheinandaloeemodininrhubarb
AT bozhang developmentofacolloidalgoldimmunochromatographicstripfortheonestepevaluationofthetotalcontentofrheinandaloeemodininrhubarb
AT yanjuanliu developmentofacolloidalgoldimmunochromatographicstripfortheonestepevaluationofthetotalcontentofrheinandaloeemodininrhubarb