Intracellular Formation of Synthetic Peptide Nanostructures Causes Mitochondrial Disruption and Cell Death in Tumor Spheroids
Abstract Supramolecular assemblies found in nature demonstrate the concept of creating functionality through structure formation. In recent years, these complex natural architectures have inspired the development of materials for the formation of synthetic nanostructures within living cells. These i...
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| Format: | Article |
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Wiley
2025-07-01
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| Series: | Advanced Science |
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| Online Access: | https://doi.org/10.1002/advs.202412606 |
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| author | Sarah Chagri Konrad Maxeiner Maria J. S. A. Silva Lisa Förch Julian Link Patrick Roth Raphael Meyer Jana Fetzer Anke Kaltbeitzel Ingo Lieberwirth Katharina Landfester Manfred Wagner David Y.W. Ng Tanja Weil |
| author_facet | Sarah Chagri Konrad Maxeiner Maria J. S. A. Silva Lisa Förch Julian Link Patrick Roth Raphael Meyer Jana Fetzer Anke Kaltbeitzel Ingo Lieberwirth Katharina Landfester Manfred Wagner David Y.W. Ng Tanja Weil |
| author_sort | Sarah Chagri |
| collection | DOAJ |
| description | Abstract Supramolecular assemblies found in nature demonstrate the concept of creating functionality through structure formation. In recent years, these complex natural architectures have inspired the development of materials for the formation of synthetic nanostructures within living cells. These intracellular assemblies have the potential to modulate cellular processes, yet their specific effects on cellular metabolism and 3D cell networks, such as tumor spheroids, still remain underexplored. Herein, the study correlates the glutathione‐induced formation of synthetic nanostructures inside MDA‐MB‐231 triple‐negative breast cancer cells to the metabolic disruption and mitochondrial degradation observed in 2D cell culture, as well as to cell death and size decrease in a 3D tumor spheroid model. In 2D cell culture, material‐cell interactions are examined through live‐cell imaging and by quantifying changes in mitochondrial respiration. By studying the interplay between glutathione‐responsive cytosolic peptide assembly and the implications on the integrity of the mitochondrial network, as well as on 3D cell networks, the work advances the understanding of how synthetic intracellular nanofibers impact vital functions of living cells. |
| format | Article |
| id | doaj-art-46d5134bb6ef489e95f6bcce7d8bbf8e |
| institution | DOAJ |
| issn | 2198-3844 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advanced Science |
| spelling | doaj-art-46d5134bb6ef489e95f6bcce7d8bbf8e2025-08-20T03:15:35ZengWileyAdvanced Science2198-38442025-07-011225n/an/a10.1002/advs.202412606Intracellular Formation of Synthetic Peptide Nanostructures Causes Mitochondrial Disruption and Cell Death in Tumor SpheroidsSarah Chagri0Konrad Maxeiner1Maria J. S. A. Silva2Lisa Förch3Julian Link4Patrick Roth5Raphael Meyer6Jana Fetzer7Anke Kaltbeitzel8Ingo Lieberwirth9Katharina Landfester10Manfred Wagner11David Y.W. Ng12Tanja Weil13Max Planck Institute for Polymer Research 55128 Mainz GermanyMax Planck Institute for Polymer Research 55128 Mainz GermanyMax Planck Institute for Polymer Research 55128 Mainz GermanyMax Planck Institute for Polymer Research 55128 Mainz GermanyMax Planck Institute for Polymer Research 55128 Mainz GermanyMax Planck Institute for Polymer Research 55128 Mainz GermanyMax Planck Institute for Polymer Research 55128 Mainz GermanyMax Planck Institute for Polymer Research 55128 Mainz GermanyMax Planck Institute for Polymer Research 55128 Mainz GermanyMax Planck Institute for Polymer Research 55128 Mainz GermanyMax Planck Institute for Polymer Research 55128 Mainz GermanyMax Planck Institute for Polymer Research 55128 Mainz GermanyMax Planck Institute for Polymer Research 55128 Mainz GermanyMax Planck Institute for Polymer Research 55128 Mainz GermanyAbstract Supramolecular assemblies found in nature demonstrate the concept of creating functionality through structure formation. In recent years, these complex natural architectures have inspired the development of materials for the formation of synthetic nanostructures within living cells. These intracellular assemblies have the potential to modulate cellular processes, yet their specific effects on cellular metabolism and 3D cell networks, such as tumor spheroids, still remain underexplored. Herein, the study correlates the glutathione‐induced formation of synthetic nanostructures inside MDA‐MB‐231 triple‐negative breast cancer cells to the metabolic disruption and mitochondrial degradation observed in 2D cell culture, as well as to cell death and size decrease in a 3D tumor spheroid model. In 2D cell culture, material‐cell interactions are examined through live‐cell imaging and by quantifying changes in mitochondrial respiration. By studying the interplay between glutathione‐responsive cytosolic peptide assembly and the implications on the integrity of the mitochondrial network, as well as on 3D cell networks, the work advances the understanding of how synthetic intracellular nanofibers impact vital functions of living cells.https://doi.org/10.1002/advs.202412606bioresponsive nanomaterialsmitochondrial disruptionpeptide nanostructuressupramolecular assembliessynthetic intracellular nanostructurestumor spheroids |
| spellingShingle | Sarah Chagri Konrad Maxeiner Maria J. S. A. Silva Lisa Förch Julian Link Patrick Roth Raphael Meyer Jana Fetzer Anke Kaltbeitzel Ingo Lieberwirth Katharina Landfester Manfred Wagner David Y.W. Ng Tanja Weil Intracellular Formation of Synthetic Peptide Nanostructures Causes Mitochondrial Disruption and Cell Death in Tumor Spheroids Advanced Science bioresponsive nanomaterials mitochondrial disruption peptide nanostructures supramolecular assemblies synthetic intracellular nanostructures tumor spheroids |
| title | Intracellular Formation of Synthetic Peptide Nanostructures Causes Mitochondrial Disruption and Cell Death in Tumor Spheroids |
| title_full | Intracellular Formation of Synthetic Peptide Nanostructures Causes Mitochondrial Disruption and Cell Death in Tumor Spheroids |
| title_fullStr | Intracellular Formation of Synthetic Peptide Nanostructures Causes Mitochondrial Disruption and Cell Death in Tumor Spheroids |
| title_full_unstemmed | Intracellular Formation of Synthetic Peptide Nanostructures Causes Mitochondrial Disruption and Cell Death in Tumor Spheroids |
| title_short | Intracellular Formation of Synthetic Peptide Nanostructures Causes Mitochondrial Disruption and Cell Death in Tumor Spheroids |
| title_sort | intracellular formation of synthetic peptide nanostructures causes mitochondrial disruption and cell death in tumor spheroids |
| topic | bioresponsive nanomaterials mitochondrial disruption peptide nanostructures supramolecular assemblies synthetic intracellular nanostructures tumor spheroids |
| url | https://doi.org/10.1002/advs.202412606 |
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