The updated duplex fluorescence quantitative RT-PCR assay for simultaneous detection of PRRSV-1 and PRRSV-2

The updated duplex fluorescence quantitative RT-PCR assay for simultaneous detection of PRRSV-1 and PRRSV-2

IntroductionPorcine reproductive and respiratory syndrome (PRRS) is one of the most economically devastating infectious diseases in the global swine industry. With the continuous mutation and recombination of PRRSV, existing detection methods frequently result in false negatives, further complicatin...

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Bibliographic Details
Main Authors:	Xiaoxiao Tian, Haojie Wang, Zeqing Liu, Ziyi Wei, Yongbo Yang, Haiwei Wang, Guoqing Liu, Hao Song, Xinyi Huang, Tongqing An
Format:	Article
Language:	English
Published:	Frontiers Media S.A. 2025-06-01
Series:	Frontiers in Cellular and Infection Microbiology
Subjects:	PRRSV-1 PRRSV-2 duplex fluorescence quantitative RT-PCR swine detection
Online Access:	https://www.frontiersin.org/articles/10.3389/fcimb.2025.1616898/full
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