C-Reactive Protein Knockout Attenuates Temporomandibular Joint Inflammation in Rats
Background. C-reactive protein (CRP), a biomarker of inflammation, is highly expressed in osteoarthritis- (OA-) related diseases, but its exact role remains unknown. In this study, we evaluated the biological effect of CRP on temporomandibular joint (TMJ) inflammation. Methods. Freund’s complete adj...
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| Format: | Article |
| Language: | English |
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Wiley
2022-01-01
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| Series: | Journal of Immunology Research |
| Online Access: | http://dx.doi.org/10.1155/2022/8613986 |
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| author | Yao He Mengjiao Zhou Zixiang Jian Lingli Fang Lan Huang Jinlin Song |
| author_facet | Yao He Mengjiao Zhou Zixiang Jian Lingli Fang Lan Huang Jinlin Song |
| author_sort | Yao He |
| collection | DOAJ |
| description | Background. C-reactive protein (CRP), a biomarker of inflammation, is highly expressed in osteoarthritis- (OA-) related diseases, but its exact role remains unknown. In this study, we evaluated the biological effect of CRP on temporomandibular joint (TMJ) inflammation. Methods. Freund’s complete adjuvant (CFA) was used to induce TMJ inflammation in CRP-knockout (CRP-/-) and control rats. Degenerative changes in the TMJ were compared to elucidate the role of CRP in TMJ inflammation. In addition, inflammatory cytokines, macrophage activation, and osteoclast differentiation were evaluated by real-time quantitative polymerase chain reaction, immunohistochemistry, and tartrate-resistant phosphatase staining to explore the potential regulatory mechanism. Results. Compared to the control, CFA induced TMJ inflammation, which increased systemic and local CRP expression. Furthermore, CRP-/- rats exhibited less severe inflammatory symptoms. The downregulation of proinflammatory cytokines (interleukin- (IL-) 1β and IL-6) and upregulation of the anti-inflammatory cytokine IL-10 were detected in CRP-/- rats, which also exhibited reduced macrophage activation and osteoclast differentiation. Conclusion. These results indicated that controlling the highly elevated levels of CRP during inflammation could modify the cytokine profile, macrophage activation, and osteoclast differentiation, thus, providing beneficial effects for TMJ-OA prevention and treatment. |
| format | Article |
| id | doaj-art-46cd9313b5b14fd19cd20876c8eb5ff0 |
| institution | Kabale University |
| issn | 2314-7156 |
| language | English |
| publishDate | 2022-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Immunology Research |
| spelling | doaj-art-46cd9313b5b14fd19cd20876c8eb5ff02025-02-03T06:05:31ZengWileyJournal of Immunology Research2314-71562022-01-01202210.1155/2022/8613986C-Reactive Protein Knockout Attenuates Temporomandibular Joint Inflammation in RatsYao He0Mengjiao Zhou1Zixiang Jian2Lingli Fang3Lan Huang4Jinlin Song5Department of OrthodonticsDepartment of OrthodonticsDepartment of OrthodonticsDepartment of OrthodonticsDepartment of OrthodonticsDepartment of OrthodonticsBackground. C-reactive protein (CRP), a biomarker of inflammation, is highly expressed in osteoarthritis- (OA-) related diseases, but its exact role remains unknown. In this study, we evaluated the biological effect of CRP on temporomandibular joint (TMJ) inflammation. Methods. Freund’s complete adjuvant (CFA) was used to induce TMJ inflammation in CRP-knockout (CRP-/-) and control rats. Degenerative changes in the TMJ were compared to elucidate the role of CRP in TMJ inflammation. In addition, inflammatory cytokines, macrophage activation, and osteoclast differentiation were evaluated by real-time quantitative polymerase chain reaction, immunohistochemistry, and tartrate-resistant phosphatase staining to explore the potential regulatory mechanism. Results. Compared to the control, CFA induced TMJ inflammation, which increased systemic and local CRP expression. Furthermore, CRP-/- rats exhibited less severe inflammatory symptoms. The downregulation of proinflammatory cytokines (interleukin- (IL-) 1β and IL-6) and upregulation of the anti-inflammatory cytokine IL-10 were detected in CRP-/- rats, which also exhibited reduced macrophage activation and osteoclast differentiation. Conclusion. These results indicated that controlling the highly elevated levels of CRP during inflammation could modify the cytokine profile, macrophage activation, and osteoclast differentiation, thus, providing beneficial effects for TMJ-OA prevention and treatment.http://dx.doi.org/10.1155/2022/8613986 |
| spellingShingle | Yao He Mengjiao Zhou Zixiang Jian Lingli Fang Lan Huang Jinlin Song C-Reactive Protein Knockout Attenuates Temporomandibular Joint Inflammation in Rats Journal of Immunology Research |
| title | C-Reactive Protein Knockout Attenuates Temporomandibular Joint Inflammation in Rats |
| title_full | C-Reactive Protein Knockout Attenuates Temporomandibular Joint Inflammation in Rats |
| title_fullStr | C-Reactive Protein Knockout Attenuates Temporomandibular Joint Inflammation in Rats |
| title_full_unstemmed | C-Reactive Protein Knockout Attenuates Temporomandibular Joint Inflammation in Rats |
| title_short | C-Reactive Protein Knockout Attenuates Temporomandibular Joint Inflammation in Rats |
| title_sort | c reactive protein knockout attenuates temporomandibular joint inflammation in rats |
| url | http://dx.doi.org/10.1155/2022/8613986 |
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