Towards a comprehensive view of wetland benthic communities
Benthic prokaryotic communities, utmost important for wetlands and marine environments functioning, are influenced by physical-chemical parameters and interactions with other communities, especially micro-eukaryotes and meiofauna. Thus, a holistic view of the benthic community is necessary to fully...
Saved in:
| Main Authors: | , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-01-01
|
| Series: | Current Research in Microbial Sciences |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2666517425000537 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850213218184593408 |
|---|---|
| author | Clélia Duran Christine Dupuy Hélène Agogué Robert Duran Cristiana Cravo-Laureau |
| author_facet | Clélia Duran Christine Dupuy Hélène Agogué Robert Duran Cristiana Cravo-Laureau |
| author_sort | Clélia Duran |
| collection | DOAJ |
| description | Benthic prokaryotic communities, utmost important for wetlands and marine environments functioning, are influenced by physical-chemical parameters and interactions with other communities, especially micro-eukaryotes and meiofauna. Thus, a holistic view of the benthic community is necessary to fully understand their organization and functioning. This study assesses the implementation of a comprehensive view, using mock communities and environmental samples. A DNA extraction strategy combining two procedures is proposed: one to obtain DNA from micro-organisms, using 0.25 g of sediment, and the other from meiofauna, using 0.25 g of sieving refluxes from 5 g of sediment. Three conditions were considered to create mock communities: (i) varying eukaryotes’ abundance, (ii) adding meiofauna from salted or freshwater wetlands, and (iii) including or not a sediment matrix. Most organisms composing the mock communities were detected, except a filamentous cyanobacteria. All mock communities showed similar composition indicating that sediment did not affect the DNA extraction. This result also demonstrated that sieving, necessary to enrich meiofauna from sediment, does not significantly affect any of the communities. For the environmental samples investigated, most of the taxa usually described in the literature were retrieved in the salted, brackish and freshwater marshes sediment. The proposed approach was successful in analysing organisms from the three domains of life in a unique environmental sample, providing a holistic view of the benthic community. Furthermore, the significant differences observed between samples from the three different marshes, indicated that our approach can be used for conducting successful ecological studies, especially useful for understanding benthic communities’ interactions. |
| format | Article |
| id | doaj-art-46c92ac9b82c4ebaaeda559d8e68b99d |
| institution | OA Journals |
| issn | 2666-5174 |
| language | English |
| publishDate | 2025-01-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Current Research in Microbial Sciences |
| spelling | doaj-art-46c92ac9b82c4ebaaeda559d8e68b99d2025-08-20T02:09:11ZengElsevierCurrent Research in Microbial Sciences2666-51742025-01-01810039110.1016/j.crmicr.2025.100391Towards a comprehensive view of wetland benthic communitiesClélia Duran0Christine Dupuy1Hélène Agogué2Robert Duran3Cristiana Cravo-Laureau4Universite de Pau et des Pays de l'Adour, UPPA, CNRS, IPREM, Pau, France; UMR 7266 LIENSs (Littoral Environnement et Sociétés), CNRS - La Rochelle Université, La Rochelle, FranceUMR 7266 LIENSs (Littoral Environnement et Sociétés), CNRS - La Rochelle Université, La Rochelle, FranceUMR 7266 LIENSs (Littoral Environnement et Sociétés), CNRS - La Rochelle Université, La Rochelle, FranceUniversite de Pau et des Pays de l'Adour, UPPA, CNRS, IPREM, Pau, FranceUniversite de Pau et des Pays de l'Adour, UPPA, CNRS, IPREM, Pau, France; Corresponding author at: Université de Pau, Avenue de l'Université, Bâtiment IBEAS, BP 1155 - 64013 PAU CEDEX, France.Benthic prokaryotic communities, utmost important for wetlands and marine environments functioning, are influenced by physical-chemical parameters and interactions with other communities, especially micro-eukaryotes and meiofauna. Thus, a holistic view of the benthic community is necessary to fully understand their organization and functioning. This study assesses the implementation of a comprehensive view, using mock communities and environmental samples. A DNA extraction strategy combining two procedures is proposed: one to obtain DNA from micro-organisms, using 0.25 g of sediment, and the other from meiofauna, using 0.25 g of sieving refluxes from 5 g of sediment. Three conditions were considered to create mock communities: (i) varying eukaryotes’ abundance, (ii) adding meiofauna from salted or freshwater wetlands, and (iii) including or not a sediment matrix. Most organisms composing the mock communities were detected, except a filamentous cyanobacteria. All mock communities showed similar composition indicating that sediment did not affect the DNA extraction. This result also demonstrated that sieving, necessary to enrich meiofauna from sediment, does not significantly affect any of the communities. For the environmental samples investigated, most of the taxa usually described in the literature were retrieved in the salted, brackish and freshwater marshes sediment. The proposed approach was successful in analysing organisms from the three domains of life in a unique environmental sample, providing a holistic view of the benthic community. Furthermore, the significant differences observed between samples from the three different marshes, indicated that our approach can be used for conducting successful ecological studies, especially useful for understanding benthic communities’ interactions.http://www.sciencedirect.com/science/article/pii/S2666517425000537Benthic community diversityMetabarcodingMarshesCoastal sedimentMock community |
| spellingShingle | Clélia Duran Christine Dupuy Hélène Agogué Robert Duran Cristiana Cravo-Laureau Towards a comprehensive view of wetland benthic communities Current Research in Microbial Sciences Benthic community diversity Metabarcoding Marshes Coastal sediment Mock community |
| title | Towards a comprehensive view of wetland benthic communities |
| title_full | Towards a comprehensive view of wetland benthic communities |
| title_fullStr | Towards a comprehensive view of wetland benthic communities |
| title_full_unstemmed | Towards a comprehensive view of wetland benthic communities |
| title_short | Towards a comprehensive view of wetland benthic communities |
| title_sort | towards a comprehensive view of wetland benthic communities |
| topic | Benthic community diversity Metabarcoding Marshes Coastal sediment Mock community |
| url | http://www.sciencedirect.com/science/article/pii/S2666517425000537 |
| work_keys_str_mv | AT cleliaduran towardsacomprehensiveviewofwetlandbenthiccommunities AT christinedupuy towardsacomprehensiveviewofwetlandbenthiccommunities AT heleneagogue towardsacomprehensiveviewofwetlandbenthiccommunities AT robertduran towardsacomprehensiveviewofwetlandbenthiccommunities AT cristianacravolaureau towardsacomprehensiveviewofwetlandbenthiccommunities |