Efficacy of human heparin-binding EGF-like growth factor in healing experimental chemical burns
According to the WHO, burns are the third most common cause of traumatic skin injuries. Chemical burns are characterized by a complex course and a long healing process. The use of the representatives of the epidermal growth factor family seems to be a promising direction in the treatment of chemical...
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National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry
2025-04-01
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| Series: | The Ukrainian Biochemical Journal |
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| Online Access: | http://ukrbiochemjournal.org/wp-content/uploads/2025/05/Dronko_97_2.pdf |
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| author | L. M. Dronko T. M. Lutsenko O. I. Golembiovska T. Yu. Trokhymchuk M. A. Arkhypova V. A. Dibrova Yu. V. Dibrova S. L. Rybalko S. A. Myakushko A. A. Siromolot O. Yu. Galkin |
| author_facet | L. M. Dronko T. M. Lutsenko O. I. Golembiovska T. Yu. Trokhymchuk M. A. Arkhypova V. A. Dibrova Yu. V. Dibrova S. L. Rybalko S. A. Myakushko A. A. Siromolot O. Yu. Galkin |
| author_sort | L. M. Dronko |
| collection | DOAJ |
| description | According to the WHO, burns are the third most common cause of traumatic skin injuries. Chemical burns are characterized by a complex course and a long healing process. The use of the representatives of the epidermal growth factor family seems to be a promising direction in the treatment of chemical burns. The aim of this study was to evaluate the effectiveness of the recombinant human heparin-binding EGF-like growth factor (rhHB-EGF) in treating burn wounds in mice. The expression of a recombinant human HB-EGF analog was induced in the prokaryotic system E. coli BL21 Star, the protein was isolated, purified and its preparations in PBS or in a form of gel containing sodium hyaluronate and potassium sorbate were obtained. The burn wound was simulated in white non-inbred mice by subcutaneous injection of 10% paraformaldehyde solution, healing was observed for 17 days. The initial wound area was measured on the 7th day after the burn injury when the treatment was started. The mice with burn injury were divided into groups of 3 each – untreated mice (control), treated with different preparations: gel without rhHB-EGF; rhHB-EGF in a gel form; rhHB-EGF in PBS solution. The preparations (100 μl, 1.5 mg of rhHB-EGF) were applied to burn wounds daily for 5 days. Wound area, healing rate and histological patterns of skin samples were estimated. It was shown that groups with burn injury treated with HB-EGF protein (both in solution and gel form) demonstrated an advantage in reducing the wound area and inflammatory cells infiltration, improving healing rate, increasing the proliferative activity of epithelial cells and neovascularization as compared with the untreated group. Thus, the use of rhHB-EGF is a promising direction in the treatment of skin wounds. |
| format | Article |
| id | doaj-art-46bafaf951424514afda3106f5b2fcd8 |
| institution | Kabale University |
| issn | 2409-4943 2413-5003 |
| language | English |
| publishDate | 2025-04-01 |
| publisher | National Academy of Sciences of Ukraine, Palladin Institute of Biochemistry |
| record_format | Article |
| series | The Ukrainian Biochemical Journal |
| spelling | doaj-art-46bafaf951424514afda3106f5b2fcd82025-08-20T03:25:12ZengNational Academy of Sciences of Ukraine, Palladin Institute of BiochemistryThe Ukrainian Biochemical Journal2409-49432413-50032025-04-01972314010.15407/ubj97.02.031Efficacy of human heparin-binding EGF-like growth factor in healing experimental chemical burnsL. M. Dronko 0T. M. Lutsenko 1https://orcid.org/0000-0002-6023-0428O. I. Golembiovska2https://orcid.org/0000-0001-5531-5374T. Yu. Trokhymchuk 3M. A. Arkhypova 4https://orcid.org/0000-0001-5860-2870V. A. Dibrova5https://orcid.org/0000-0003-3452-6257 Yu. V. Dibrova6S. L. Rybalko7https://orcid.org/0000-0002-1913-1380 S. A. Myakushko 8A. A. Siromolot 9https://orcid.org/0000-0002-2180-3545O. Yu. Galkin10https://orcid.org/0000-0002-5309-6099National Technical University of Ukraine “Igor Sikorsky Kyiv Polytechnic Institute”, Kyiv, UkraineNational Technical University of Ukraine “Igor Sikorsky Kyiv Polytechnic Institute”, Kyiv, UkraineNational Technical University of Ukraine “Igor Sikorsky Kyiv Polytechnic Institute”, Kyiv, UkraineSІ “L.V. Gromashevskyi Institute of Epidemiology and Infectious Diseases of the NAMS of Ukraine”, Kyiv, UkraineSІ “L.V. Gromashevskyi Institute of Epidemiology and Infectious Diseases of the NAMS of Ukraine”, Kyiv, UkraineSІ “L.V. Gromashevskyi Institute of Epidemiology and Infectious Diseases of the NAMS of Ukraine”, Kyiv, UkraineSІ “L.V. Gromashevskyi Institute of Epidemiology and Infectious Diseases of the NAMS of Ukraine”, Kyiv, UkraineSІ “L.V. Gromashevskyi Institute of Epidemiology and Infectious Diseases of the NAMS of Ukraine”, Kyiv, UkraineESC “Institute of Biology and Medicine”, Taras Shevchenko National University of Kyiv, UkraineESC “Institute of Biology and Medicine”, Taras Shevchenko National University of Kyiv, Ukraine; Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv, Ukraine National Technical University of Ukraine “Igor Sikorsky Kyiv Polytechnic Institute”, Kyiv, Ukraine; Palladin Institute of Biochemistry, National Academy of Sciences of Ukraine, Kyiv, UkraineAccording to the WHO, burns are the third most common cause of traumatic skin injuries. Chemical burns are characterized by a complex course and a long healing process. The use of the representatives of the epidermal growth factor family seems to be a promising direction in the treatment of chemical burns. The aim of this study was to evaluate the effectiveness of the recombinant human heparin-binding EGF-like growth factor (rhHB-EGF) in treating burn wounds in mice. The expression of a recombinant human HB-EGF analog was induced in the prokaryotic system E. coli BL21 Star, the protein was isolated, purified and its preparations in PBS or in a form of gel containing sodium hyaluronate and potassium sorbate were obtained. The burn wound was simulated in white non-inbred mice by subcutaneous injection of 10% paraformaldehyde solution, healing was observed for 17 days. The initial wound area was measured on the 7th day after the burn injury when the treatment was started. The mice with burn injury were divided into groups of 3 each – untreated mice (control), treated with different preparations: gel without rhHB-EGF; rhHB-EGF in a gel form; rhHB-EGF in PBS solution. The preparations (100 μl, 1.5 mg of rhHB-EGF) were applied to burn wounds daily for 5 days. Wound area, healing rate and histological patterns of skin samples were estimated. It was shown that groups with burn injury treated with HB-EGF protein (both in solution and gel form) demonstrated an advantage in reducing the wound area and inflammatory cells infiltration, improving healing rate, increasing the proliferative activity of epithelial cells and neovascularization as compared with the untreated group. Thus, the use of rhHB-EGF is a promising direction in the treatment of skin wounds.http://ukrbiochemjournal.org/wp-content/uploads/2025/05/Dronko_97_2.pdfchemical burnsheparin-binding egf-like growth factorhistological analysisskin regenerationwound healing |
| spellingShingle | L. M. Dronko T. M. Lutsenko O. I. Golembiovska T. Yu. Trokhymchuk M. A. Arkhypova V. A. Dibrova Yu. V. Dibrova S. L. Rybalko S. A. Myakushko A. A. Siromolot O. Yu. Galkin Efficacy of human heparin-binding EGF-like growth factor in healing experimental chemical burns The Ukrainian Biochemical Journal chemical burns heparin-binding egf-like growth factor histological analysis skin regeneration wound healing |
| title | Efficacy of human heparin-binding EGF-like growth factor in healing experimental chemical burns |
| title_full | Efficacy of human heparin-binding EGF-like growth factor in healing experimental chemical burns |
| title_fullStr | Efficacy of human heparin-binding EGF-like growth factor in healing experimental chemical burns |
| title_full_unstemmed | Efficacy of human heparin-binding EGF-like growth factor in healing experimental chemical burns |
| title_short | Efficacy of human heparin-binding EGF-like growth factor in healing experimental chemical burns |
| title_sort | efficacy of human heparin binding egf like growth factor in healing experimental chemical burns |
| topic | chemical burns heparin-binding egf-like growth factor histological analysis skin regeneration wound healing |
| url | http://ukrbiochemjournal.org/wp-content/uploads/2025/05/Dronko_97_2.pdf |
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