An isothermal titration calorimetry method for monitoring the removal of ethylenediamine tetra-acetic acid from apo-proteins

Superbug infection caused by New Delhi metallo-β-lactamase (NDM-1) has grown into an emerging threat. EDTA is one of the most commonly used Zn(II) clelators for preparation of apo-metallo-β-lactamases (apo-MβLs) and further Co(II)-substituted MβLs for spectroscopic characterization of the enzymes. H...

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Bibliographic Details
Main Authors: Ying Ge, Wen-Ming Wang, Tingjun Liu
Format: Article
Language:English
Published: Elsevier 2025-07-01
Series:Results in Chemistry
Subjects:
Online Access:http://www.sciencedirect.com/science/article/pii/S221171562500356X
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