Synthesis and Application of Nanomagnetic Immobilized Phospholipase C
The nanomagnetic carrier (Fe3O4@SiO2@p(GMA)) was prepared by atom transfer radical polymerization, and then, the free phospholipase C (PLC) was immobilized on it proved by the results of FT-IR analysis. The enzyme loading was 135.64 mg/g, the enzyme activity was 8560.7 U/g, the average particle size...
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| Main Authors: | , , , , , , , |
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Journal of Chemistry |
| Online Access: | http://dx.doi.org/10.1155/2019/5951793 |
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| _version_ | 1849406967202709504 |
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| author | Yang Jiang Jing Du Honglin Tang Xin Zhang Wenbin Li Liqi Wang Lianzhou Jiang Dianyu Yu |
| author_facet | Yang Jiang Jing Du Honglin Tang Xin Zhang Wenbin Li Liqi Wang Lianzhou Jiang Dianyu Yu |
| author_sort | Yang Jiang |
| collection | DOAJ |
| description | The nanomagnetic carrier (Fe3O4@SiO2@p(GMA)) was prepared by atom transfer radical polymerization, and then, the free phospholipase C (PLC) was immobilized on it proved by the results of FT-IR analysis. The enzyme loading was 135.64 mg/g, the enzyme activity was 8560.7 U/g, the average particle size was 99.86 ± 0.80 nm, and the specific saturation magnetization was 16.00 ± 0.20 emu/g. PLC-Fe3O4@SiO2@p(GMA) showed the highest activities at the pH of 7.5, and tolerance temperature raised to 65°C in soybean lecithin emulsion. Enzymatic degumming with PLC-Fe3O4@SiO2@p(GMA) under the conditions of the enzyme dosage of 110 mg/kg, reaction temperature of 65°C, pH of 7.5, and reaction time of 2.5 h resulted in residual phosphorus of 64.7 mg/kg, 1,2-diacylglycerol (1,2-DAG) contents of 1.07%, and oil yield of 98.1%. Moreover, PLC-Fe3O4@SiO2@p(GMA) still possessed more than 80% of its initial activity after 5 cycles. |
| format | Article |
| id | doaj-art-4696a040f8ba459bb9b1c8e5af82bdab |
| institution | Kabale University |
| issn | 2090-9063 2090-9071 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Journal of Chemistry |
| spelling | doaj-art-4696a040f8ba459bb9b1c8e5af82bdab2025-08-20T03:36:13ZengWileyJournal of Chemistry2090-90632090-90712019-01-01201910.1155/2019/59517935951793Synthesis and Application of Nanomagnetic Immobilized Phospholipase CYang Jiang0Jing Du1Honglin Tang2Xin Zhang3Wenbin Li4Liqi Wang5Lianzhou Jiang6Dianyu Yu7School of Food Science, Northeast Agricultural University, Harbin 150030, ChinaThe Key Laboratory of Soybean Biology in Chinese Ministry of Education, Northeast Agricultural University, Harbin 150030, ChinaSchool of Food Science, Northeast Agricultural University, Harbin 150030, ChinaSchool of Food Science, Northeast Agricultural University, Harbin 150030, ChinaThe Key Laboratory of Soybean Biology in Chinese Ministry of Education, Northeast Agricultural University, Harbin 150030, ChinaSchool of Computer and Information Engineering, Harbin University of Commerce, Harbin 150028, ChinaSchool of Food Science, Northeast Agricultural University, Harbin 150030, ChinaSchool of Food Science, Northeast Agricultural University, Harbin 150030, ChinaThe nanomagnetic carrier (Fe3O4@SiO2@p(GMA)) was prepared by atom transfer radical polymerization, and then, the free phospholipase C (PLC) was immobilized on it proved by the results of FT-IR analysis. The enzyme loading was 135.64 mg/g, the enzyme activity was 8560.7 U/g, the average particle size was 99.86 ± 0.80 nm, and the specific saturation magnetization was 16.00 ± 0.20 emu/g. PLC-Fe3O4@SiO2@p(GMA) showed the highest activities at the pH of 7.5, and tolerance temperature raised to 65°C in soybean lecithin emulsion. Enzymatic degumming with PLC-Fe3O4@SiO2@p(GMA) under the conditions of the enzyme dosage of 110 mg/kg, reaction temperature of 65°C, pH of 7.5, and reaction time of 2.5 h resulted in residual phosphorus of 64.7 mg/kg, 1,2-diacylglycerol (1,2-DAG) contents of 1.07%, and oil yield of 98.1%. Moreover, PLC-Fe3O4@SiO2@p(GMA) still possessed more than 80% of its initial activity after 5 cycles.http://dx.doi.org/10.1155/2019/5951793 |
| spellingShingle | Yang Jiang Jing Du Honglin Tang Xin Zhang Wenbin Li Liqi Wang Lianzhou Jiang Dianyu Yu Synthesis and Application of Nanomagnetic Immobilized Phospholipase C Journal of Chemistry |
| title | Synthesis and Application of Nanomagnetic Immobilized Phospholipase C |
| title_full | Synthesis and Application of Nanomagnetic Immobilized Phospholipase C |
| title_fullStr | Synthesis and Application of Nanomagnetic Immobilized Phospholipase C |
| title_full_unstemmed | Synthesis and Application of Nanomagnetic Immobilized Phospholipase C |
| title_short | Synthesis and Application of Nanomagnetic Immobilized Phospholipase C |
| title_sort | synthesis and application of nanomagnetic immobilized phospholipase c |
| url | http://dx.doi.org/10.1155/2019/5951793 |
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