Optimized conditions for extraction, quantification and detection of bioactive compound from Nettle (Urtica dioica L.) using the deep eutectic solvents, ultra-sonication and liquid chromatography-mass spectrometry (LC-DAD-ESI-MS/MS)
The bioactive constituents of Nettle (Urtica dioica L.) plants were extracted by green and environmentally friendly methods using several deep eutectic solvents (DESs) followed by liquid chromatography-mass spectrometry separation and identification. To optimize the extraction process, bio-solvents...
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| Format: | Article |
| Language: | English |
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Taylor & Francis Group
2023-09-01
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| Series: | International Journal of Food Properties |
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| Online Access: | https://www.tandfonline.com/doi/10.1080/10942912.2023.2244194 |
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| author | Hyrije Koraqi Bujar Qazimi Waseem Khalid Jasmina Petreska Stanoeva Aqeela Sehrish Farzana Siddique Cengiz Çesko Khalid Ali Khan Muhammad Abdul Rahim Imtiaz Hussain Eliasse Zongo |
| author_facet | Hyrije Koraqi Bujar Qazimi Waseem Khalid Jasmina Petreska Stanoeva Aqeela Sehrish Farzana Siddique Cengiz Çesko Khalid Ali Khan Muhammad Abdul Rahim Imtiaz Hussain Eliasse Zongo |
| author_sort | Hyrije Koraqi |
| collection | DOAJ |
| description | The bioactive constituents of Nettle (Urtica dioica L.) plants were extracted by green and environmentally friendly methods using several deep eutectic solvents (DESs) followed by liquid chromatography-mass spectrometry separation and identification. To optimize the extraction process, bio-solvents composed of organic acids such as hydrogen bond donors (HBD) and mannitol/menthol/sugars as HBA were selected and coupled with ultrasound-assisted extraction. The maximum bioactive compound contents were TPC 2423 mg GAE/100 g, TFC 134.71 mg CE/100 g, and DPPH 1071.05 mol TE/mL. The optimum extraction parameters to achieve the highest values of phenolic compounds from Nettle (Urtica dioica L.) via UAE-DES were obtained at an extraction temperature 70°C, extraction time of 30 min, and water addition on DES 20%. The ideal experimental conditions were confirmed using the Central Composite Design (CCD) of the Response Surface Methodology (RSM) model to analyze the significance of extraction parameters demonstrating high prediction levels of the bioactive compounds. Liquid chromatography coupled with diode array detection and electrospray ionization tandem mass spectrometry (LC-DAD-ESI-MS/MS) was used to identify the extracts. The results showed that quercetin-3-O-rutinoside, 5-ocaffeoylquinic acid, and caffeoyl malic acid were the three compounds with the highest abundance. For the first time, the extraction of bioactive compounds from nettle using a food-grade DES that contains GRAS components is reported. According to these findings, the best solvent for extracting bioactive substances for food-grade applications is DESCAMAL (citric acid/maltose). |
| format | Article |
| id | doaj-art-4695edb0e6564e72a15a2436b6519900 |
| institution | Kabale University |
| issn | 1094-2912 1532-2386 |
| language | English |
| publishDate | 2023-09-01 |
| publisher | Taylor & Francis Group |
| record_format | Article |
| series | International Journal of Food Properties |
| spelling | doaj-art-4695edb0e6564e72a15a2436b65199002025-01-02T10:41:35ZengTaylor & Francis GroupInternational Journal of Food Properties1094-29121532-23862023-09-012612171218510.1080/10942912.2023.2244194Optimized conditions for extraction, quantification and detection of bioactive compound from Nettle (Urtica dioica L.) using the deep eutectic solvents, ultra-sonication and liquid chromatography-mass spectrometry (LC-DAD-ESI-MS/MS)Hyrije Koraqi0Bujar Qazimi1Waseem Khalid2Jasmina Petreska Stanoeva3Aqeela Sehrish4Farzana Siddique5Cengiz Çesko6Khalid Ali Khan7Muhammad Abdul Rahim8Imtiaz Hussain9Eliasse Zongo10Faculty of Pharmacy, UBT-Higher Education Institution, Pristina, KosovoFaculty of Pharmacy, UBT-Higher Education Institution, Pristina, KosovoUniversity Institute of Food Science and Technology, The University of Lahore, Lahore, PakistanInstitute of Chemistry, Faculty of Natural Sciences and Mathematics, Ss. Cyril and Methodius University in Skopje, Skopje, Republic of North MacedoniaDepartment of Plant and Soil Science, Texas Tech University, Lubbock, USAInstitute of Food Science and Nutrition, University of Sargodha, Sargodha, PakistanFaculty of Food Science and Biotechnology, UBT-Higher Education Institution, Pristina, KosovoUnit of Bee Research and Honey Production, Research Center for Advanced Materials Science (RCAMS), King Khalid University, Abha, Saudi ArabiaDepartment of Food Science, Faculty of Life Science, Government College University Faisalabad, Faisalabad, Punjab, PakistanDepartment of Food Science and Technology, Faculty of Agriculture, University of Poonch, Rawalakot, PakistanLaboratoire de Recherche Et d’Enseignement En Santé Et Biotechnologies Animales, Université Nazi BONI, Bobo Dioulasso, Burkina FasoThe bioactive constituents of Nettle (Urtica dioica L.) plants were extracted by green and environmentally friendly methods using several deep eutectic solvents (DESs) followed by liquid chromatography-mass spectrometry separation and identification. To optimize the extraction process, bio-solvents composed of organic acids such as hydrogen bond donors (HBD) and mannitol/menthol/sugars as HBA were selected and coupled with ultrasound-assisted extraction. The maximum bioactive compound contents were TPC 2423 mg GAE/100 g, TFC 134.71 mg CE/100 g, and DPPH 1071.05 mol TE/mL. The optimum extraction parameters to achieve the highest values of phenolic compounds from Nettle (Urtica dioica L.) via UAE-DES were obtained at an extraction temperature 70°C, extraction time of 30 min, and water addition on DES 20%. The ideal experimental conditions were confirmed using the Central Composite Design (CCD) of the Response Surface Methodology (RSM) model to analyze the significance of extraction parameters demonstrating high prediction levels of the bioactive compounds. Liquid chromatography coupled with diode array detection and electrospray ionization tandem mass spectrometry (LC-DAD-ESI-MS/MS) was used to identify the extracts. The results showed that quercetin-3-O-rutinoside, 5-ocaffeoylquinic acid, and caffeoyl malic acid were the three compounds with the highest abundance. For the first time, the extraction of bioactive compounds from nettle using a food-grade DES that contains GRAS components is reported. According to these findings, the best solvent for extracting bioactive substances for food-grade applications is DESCAMAL (citric acid/maltose).https://www.tandfonline.com/doi/10.1080/10942912.2023.2244194Green extraction methoddeep eutectic solventsNettle (Urtica dioica L.)ultrasound-assisted extractioncentral composite designliquid chromatography-mass spectrometry |
| spellingShingle | Hyrije Koraqi Bujar Qazimi Waseem Khalid Jasmina Petreska Stanoeva Aqeela Sehrish Farzana Siddique Cengiz Çesko Khalid Ali Khan Muhammad Abdul Rahim Imtiaz Hussain Eliasse Zongo Optimized conditions for extraction, quantification and detection of bioactive compound from Nettle (Urtica dioica L.) using the deep eutectic solvents, ultra-sonication and liquid chromatography-mass spectrometry (LC-DAD-ESI-MS/MS) International Journal of Food Properties Green extraction method deep eutectic solvents Nettle (Urtica dioica L.) ultrasound-assisted extraction central composite design liquid chromatography-mass spectrometry |
| title | Optimized conditions for extraction, quantification and detection of bioactive compound from Nettle (Urtica dioica L.) using the deep eutectic solvents, ultra-sonication and liquid chromatography-mass spectrometry (LC-DAD-ESI-MS/MS) |
| title_full | Optimized conditions for extraction, quantification and detection of bioactive compound from Nettle (Urtica dioica L.) using the deep eutectic solvents, ultra-sonication and liquid chromatography-mass spectrometry (LC-DAD-ESI-MS/MS) |
| title_fullStr | Optimized conditions for extraction, quantification and detection of bioactive compound from Nettle (Urtica dioica L.) using the deep eutectic solvents, ultra-sonication and liquid chromatography-mass spectrometry (LC-DAD-ESI-MS/MS) |
| title_full_unstemmed | Optimized conditions for extraction, quantification and detection of bioactive compound from Nettle (Urtica dioica L.) using the deep eutectic solvents, ultra-sonication and liquid chromatography-mass spectrometry (LC-DAD-ESI-MS/MS) |
| title_short | Optimized conditions for extraction, quantification and detection of bioactive compound from Nettle (Urtica dioica L.) using the deep eutectic solvents, ultra-sonication and liquid chromatography-mass spectrometry (LC-DAD-ESI-MS/MS) |
| title_sort | optimized conditions for extraction quantification and detection of bioactive compound from nettle urtica dioica l using the deep eutectic solvents ultra sonication and liquid chromatography mass spectrometry lc dad esi ms ms |
| topic | Green extraction method deep eutectic solvents Nettle (Urtica dioica L.) ultrasound-assisted extraction central composite design liquid chromatography-mass spectrometry |
| url | https://www.tandfonline.com/doi/10.1080/10942912.2023.2244194 |
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