BMP4/LIF or RA/Forskolin Suppresses the Proliferation of Neural Stem Cells Derived from Adult Monkey Brain
Monkeys are much closer to human and are the most common nonhuman primates which are used in biomedical studies. Neural progenitor cells can originate from the hippocampus of adult monkeys. Despite a few reports, the detailed properties of monkey neural stem cells (NSCs) and their responses to cytok...
Saved in:
| Main Authors: | , , , , , , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Wiley
2017-01-01
|
| Series: | Stem Cells International |
| Online Access: | http://dx.doi.org/10.1155/2017/7012405 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1832551458865676288 |
|---|---|
| author | Xinxin Han Liming Yu Qingqing Chen Min Wang Jie Ren Guangming Wang Yihong Chen Lixia Lu Haibin Tian Li Chen Ying Zhang Yuehua Liu Hua He Zhengliang Gao |
| author_facet | Xinxin Han Liming Yu Qingqing Chen Min Wang Jie Ren Guangming Wang Yihong Chen Lixia Lu Haibin Tian Li Chen Ying Zhang Yuehua Liu Hua He Zhengliang Gao |
| author_sort | Xinxin Han |
| collection | DOAJ |
| description | Monkeys are much closer to human and are the most common nonhuman primates which are used in biomedical studies. Neural progenitor cells can originate from the hippocampus of adult monkeys. Despite a few reports, the detailed properties of monkey neural stem cells (NSCs) and their responses to cytokine are still unclear. Here, we derive NSCs from an adult monkey brain and demonstrate that BMP4 inhibits cell proliferation and affects cell morphology of monkey NSCs. Combined treatment of BMP4 and LIF or RA and Forskolin represses the proliferation of monkey NSCs. We also show that BMP4 may promote monkey NSC quiescence. Our study therefore provides implications for NSC-based cell therapy of brain injury in the future. |
| format | Article |
| id | doaj-art-46747b46803843fbb3b0af07ba4ec708 |
| institution | Kabale University |
| issn | 1687-966X 1687-9678 |
| language | English |
| publishDate | 2017-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Stem Cells International |
| spelling | doaj-art-46747b46803843fbb3b0af07ba4ec7082025-02-03T06:01:21ZengWileyStem Cells International1687-966X1687-96782017-01-01201710.1155/2017/70124057012405BMP4/LIF or RA/Forskolin Suppresses the Proliferation of Neural Stem Cells Derived from Adult Monkey BrainXinxin Han0Liming Yu1Qingqing Chen2Min Wang3Jie Ren4Guangming Wang5Yihong Chen6Lixia Lu7Haibin Tian8Li Chen9Ying Zhang10Yuehua Liu11Hua He12Zhengliang Gao13Shanghai Stomatological Hospital, Fudan University, Shanghai 200001, ChinaShanghai Stomatological Hospital, Fudan University, Shanghai 200001, ChinaShanghai Stomatological Hospital, Fudan University, Shanghai 200001, ChinaSchool of Medicine, Jiaxing University, Zhejiang 314001, ChinaShanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai 200092, ChinaShanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai 200092, ChinaChangzheng Hospital, Second Hospital Affiliated with Second Military Medical University, Shanghai 200003, ChinaShanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai 200092, ChinaShanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai 200092, ChinaShanghai Stomatological Hospital, Fudan University, Shanghai 200001, ChinaShanghai Stomatological Hospital, Fudan University, Shanghai 200001, ChinaShanghai Stomatological Hospital, Fudan University, Shanghai 200001, ChinaChangzheng Hospital, Second Hospital Affiliated with Second Military Medical University, Shanghai 200003, ChinaShanghai Tenth People’s Hospital, Tongji University School of Medicine, Shanghai 200092, ChinaMonkeys are much closer to human and are the most common nonhuman primates which are used in biomedical studies. Neural progenitor cells can originate from the hippocampus of adult monkeys. Despite a few reports, the detailed properties of monkey neural stem cells (NSCs) and their responses to cytokine are still unclear. Here, we derive NSCs from an adult monkey brain and demonstrate that BMP4 inhibits cell proliferation and affects cell morphology of monkey NSCs. Combined treatment of BMP4 and LIF or RA and Forskolin represses the proliferation of monkey NSCs. We also show that BMP4 may promote monkey NSC quiescence. Our study therefore provides implications for NSC-based cell therapy of brain injury in the future.http://dx.doi.org/10.1155/2017/7012405 |
| spellingShingle | Xinxin Han Liming Yu Qingqing Chen Min Wang Jie Ren Guangming Wang Yihong Chen Lixia Lu Haibin Tian Li Chen Ying Zhang Yuehua Liu Hua He Zhengliang Gao BMP4/LIF or RA/Forskolin Suppresses the Proliferation of Neural Stem Cells Derived from Adult Monkey Brain Stem Cells International |
| title | BMP4/LIF or RA/Forskolin Suppresses the Proliferation of Neural Stem Cells Derived from Adult Monkey Brain |
| title_full | BMP4/LIF or RA/Forskolin Suppresses the Proliferation of Neural Stem Cells Derived from Adult Monkey Brain |
| title_fullStr | BMP4/LIF or RA/Forskolin Suppresses the Proliferation of Neural Stem Cells Derived from Adult Monkey Brain |
| title_full_unstemmed | BMP4/LIF or RA/Forskolin Suppresses the Proliferation of Neural Stem Cells Derived from Adult Monkey Brain |
| title_short | BMP4/LIF or RA/Forskolin Suppresses the Proliferation of Neural Stem Cells Derived from Adult Monkey Brain |
| title_sort | bmp4 lif or ra forskolin suppresses the proliferation of neural stem cells derived from adult monkey brain |
| url | http://dx.doi.org/10.1155/2017/7012405 |
| work_keys_str_mv | AT xinxinhan bmp4liforraforskolinsuppressestheproliferationofneuralstemcellsderivedfromadultmonkeybrain AT limingyu bmp4liforraforskolinsuppressestheproliferationofneuralstemcellsderivedfromadultmonkeybrain AT qingqingchen bmp4liforraforskolinsuppressestheproliferationofneuralstemcellsderivedfromadultmonkeybrain AT minwang bmp4liforraforskolinsuppressestheproliferationofneuralstemcellsderivedfromadultmonkeybrain AT jieren bmp4liforraforskolinsuppressestheproliferationofneuralstemcellsderivedfromadultmonkeybrain AT guangmingwang bmp4liforraforskolinsuppressestheproliferationofneuralstemcellsderivedfromadultmonkeybrain AT yihongchen bmp4liforraforskolinsuppressestheproliferationofneuralstemcellsderivedfromadultmonkeybrain AT lixialu bmp4liforraforskolinsuppressestheproliferationofneuralstemcellsderivedfromadultmonkeybrain AT haibintian bmp4liforraforskolinsuppressestheproliferationofneuralstemcellsderivedfromadultmonkeybrain AT lichen bmp4liforraforskolinsuppressestheproliferationofneuralstemcellsderivedfromadultmonkeybrain AT yingzhang bmp4liforraforskolinsuppressestheproliferationofneuralstemcellsderivedfromadultmonkeybrain AT yuehualiu bmp4liforraforskolinsuppressestheproliferationofneuralstemcellsderivedfromadultmonkeybrain AT huahe bmp4liforraforskolinsuppressestheproliferationofneuralstemcellsderivedfromadultmonkeybrain AT zhenglianggao bmp4liforraforskolinsuppressestheproliferationofneuralstemcellsderivedfromadultmonkeybrain |