Unraveling Genomic and Pathogenic Features of <i>Aeromonas ichthyocola</i> sp. nov., <i>Aeromonas mytilicola</i> sp. nov., and <i>Aeromonas mytilicola</i> subsp. <i>aquatica</i> subsp. nov.
The Gram-negative genus <i>Aeromonas</i> contains diverse bacterial species that are prevalent in aquatic environments. This present study describes three novel <i>Aeromonas</i> strains: <i>A. ichthyocola</i> sp. nov. A-5<sup>T</sup> and <i>A. my...
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2025-03-01
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| author | Nihed Ajmi Muhammed Duman Batuhan Coskun Ceren Esen Oner Sonmez Gorkem Tasci Orkide Coskuner-Weber Hilal Ay Digdem Yoyen-Ermis Artun Yibar Andrew P. Desbois Izzet Burcin Saticioglu |
| author_facet | Nihed Ajmi Muhammed Duman Batuhan Coskun Ceren Esen Oner Sonmez Gorkem Tasci Orkide Coskuner-Weber Hilal Ay Digdem Yoyen-Ermis Artun Yibar Andrew P. Desbois Izzet Burcin Saticioglu |
| author_sort | Nihed Ajmi |
| collection | DOAJ |
| description | The Gram-negative genus <i>Aeromonas</i> contains diverse bacterial species that are prevalent in aquatic environments. This present study describes three novel <i>Aeromonas</i> strains: <i>A. ichthyocola</i> sp. nov. A-5<sup>T</sup> and <i>A. mytilicola</i> subsp. <i>aquatica</i> subsp. nov. A-8<sup>T</sup> isolated from rainbow trout (<i>Oncorhynchus mykiss</i>), and <i>A. mytilicola</i> sp. nov. A-7<sup>T</sup> isolated from mussels (<i>Mytilus galloprovincialis</i>), respectively. Genomic analyses revealed that strains A-5<sup>T</sup> and A-7<sup>T</sup> shared the highest <i>16S rRNA</i> gene sequence similarity with <i>A. rivipollensis</i> P2G1<sup>T</sup> (99.7% and 99.8%, respectively), while strain A-8<sup>T</sup> exhibited 99.7% identity with <i>A. media</i> RM<sup>T</sup>. Together with morphological, physiological, and biochemical data, genome-based analyses provided additional evidence for species differentiation. Digital DNA–DNA hybridization (dDDH; 56.8−65.9%) and average nucleotide identity (ANI; 94.2–95.7%) values fell below the species delineation thresholds, confirming that these isolates represent distinct taxa. Pathogenicity assays using greater wax moth (<i>Galleria mellonella</i>) larvae demonstrated strain-specific virulence profiles. Further genomic analyses identified biosynthetic gene clusters for nonribosomal peptides (NRPs) and ribosomally synthesized and post-translationally modified peptides (RiPPs), which often have roles in secondary metabolite production. Ecological analyses, based on genomic comparisons and metagenomic database searches, revealed the adaptability of the strains to diverse habitats, including freshwater, wastewater, and activated sludge. Based on the genetic and phenotypic data, the novel taxa <i>Aeromonas ichthyocola</i> sp. nov. A-5ᵀ (LMG 33534ᵀ = DSM 117488ᵀ), <i>Aeromonas mytilicola</i> sp. nov. A-7ᵀ (LMG 33536ᵀ = DSM 117490ᵀ), and <i>Aeromonas mytilicola</i> subsp. <i>aquatica</i> subsp. nov. A-8ᵀ (LMG 33537ᵀ = DSM 117493ᵀ) are proposed. |
| format | Article |
| id | doaj-art-466ef3ff01404feebe1abd97d6c9c7a4 |
| institution | DOAJ |
| issn | 2076-2615 |
| language | English |
| publishDate | 2025-03-01 |
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| spelling | doaj-art-466ef3ff01404feebe1abd97d6c9c7a42025-08-20T03:06:32ZengMDPI AGAnimals2076-26152025-03-0115794810.3390/ani15070948Unraveling Genomic and Pathogenic Features of <i>Aeromonas ichthyocola</i> sp. nov., <i>Aeromonas mytilicola</i> sp. nov., and <i>Aeromonas mytilicola</i> subsp. <i>aquatica</i> subsp. nov.Nihed Ajmi0Muhammed Duman1Batuhan Coskun2Ceren Esen3Oner Sonmez4Gorkem Tasci5Orkide Coskuner-Weber6Hilal Ay7Digdem Yoyen-Ermis8Artun Yibar9Andrew P. Desbois10Izzet Burcin Saticioglu11Department of Aquatic Animal Diseases, Graduate School of Health Science, Bursa Uludag University, Bursa 16059, TurkeyDepartment of Aquatic Animal Diseases, Faculty of Veterinary Medicine, Bursa Uludag University, Bursa 16059, TurkeyInstitute of Aquaculture, University of Stirling, Stirling FK9 4LA, UKDepartment of Immunology, Faculty of Medicine, Bursa Uludag University, Bursa 16059, TurkeyDepartment of Biochemistry, School of Veterinary Medicine, Bursa Uludag University, Bursa 16059, TurkeyDepartment of Aquatic Animal Diseases, Graduate School of Health Science, Bursa Uludag University, Bursa 16059, TurkeyMolecular Biotechnology, Turkish-German University, Sahinkaya Caddesi, No. 106, Beykoz, Istanbul 34820, TurkeyDepartment of Molecular Biology and Genetics, Faculty of Arts and Science, Yildiz Technical University, Istanbul 34220, TurkeyDepartment of Immunology, Faculty of Medicine, Bursa Uludag University, Bursa 16059, TurkeyDepartment of Food Hygiene and Technology, Faculty of Veterinary Medicine, Bursa Uludag University, Bursa 16059, TurkeyInstitute of Aquaculture, University of Stirling, Stirling FK9 4LA, UKDepartment of Aquatic Animal Diseases, Faculty of Veterinary Medicine, Bursa Uludag University, Bursa 16059, TurkeyThe Gram-negative genus <i>Aeromonas</i> contains diverse bacterial species that are prevalent in aquatic environments. This present study describes three novel <i>Aeromonas</i> strains: <i>A. ichthyocola</i> sp. nov. A-5<sup>T</sup> and <i>A. mytilicola</i> subsp. <i>aquatica</i> subsp. nov. A-8<sup>T</sup> isolated from rainbow trout (<i>Oncorhynchus mykiss</i>), and <i>A. mytilicola</i> sp. nov. A-7<sup>T</sup> isolated from mussels (<i>Mytilus galloprovincialis</i>), respectively. Genomic analyses revealed that strains A-5<sup>T</sup> and A-7<sup>T</sup> shared the highest <i>16S rRNA</i> gene sequence similarity with <i>A. rivipollensis</i> P2G1<sup>T</sup> (99.7% and 99.8%, respectively), while strain A-8<sup>T</sup> exhibited 99.7% identity with <i>A. media</i> RM<sup>T</sup>. Together with morphological, physiological, and biochemical data, genome-based analyses provided additional evidence for species differentiation. Digital DNA–DNA hybridization (dDDH; 56.8−65.9%) and average nucleotide identity (ANI; 94.2–95.7%) values fell below the species delineation thresholds, confirming that these isolates represent distinct taxa. Pathogenicity assays using greater wax moth (<i>Galleria mellonella</i>) larvae demonstrated strain-specific virulence profiles. Further genomic analyses identified biosynthetic gene clusters for nonribosomal peptides (NRPs) and ribosomally synthesized and post-translationally modified peptides (RiPPs), which often have roles in secondary metabolite production. Ecological analyses, based on genomic comparisons and metagenomic database searches, revealed the adaptability of the strains to diverse habitats, including freshwater, wastewater, and activated sludge. Based on the genetic and phenotypic data, the novel taxa <i>Aeromonas ichthyocola</i> sp. nov. A-5ᵀ (LMG 33534ᵀ = DSM 117488ᵀ), <i>Aeromonas mytilicola</i> sp. nov. A-7ᵀ (LMG 33536ᵀ = DSM 117490ᵀ), and <i>Aeromonas mytilicola</i> subsp. <i>aquatica</i> subsp. nov. A-8ᵀ (LMG 33537ᵀ = DSM 117493ᵀ) are proposed.https://www.mdpi.com/2076-2615/15/7/948<i>Aeromonas</i><i>Galleria mellonella</i>genome analysistaxonomy |
| spellingShingle | Nihed Ajmi Muhammed Duman Batuhan Coskun Ceren Esen Oner Sonmez Gorkem Tasci Orkide Coskuner-Weber Hilal Ay Digdem Yoyen-Ermis Artun Yibar Andrew P. Desbois Izzet Burcin Saticioglu Unraveling Genomic and Pathogenic Features of <i>Aeromonas ichthyocola</i> sp. nov., <i>Aeromonas mytilicola</i> sp. nov., and <i>Aeromonas mytilicola</i> subsp. <i>aquatica</i> subsp. nov. Animals <i>Aeromonas</i> <i>Galleria mellonella</i> genome analysis taxonomy |
| title | Unraveling Genomic and Pathogenic Features of <i>Aeromonas ichthyocola</i> sp. nov., <i>Aeromonas mytilicola</i> sp. nov., and <i>Aeromonas mytilicola</i> subsp. <i>aquatica</i> subsp. nov. |
| title_full | Unraveling Genomic and Pathogenic Features of <i>Aeromonas ichthyocola</i> sp. nov., <i>Aeromonas mytilicola</i> sp. nov., and <i>Aeromonas mytilicola</i> subsp. <i>aquatica</i> subsp. nov. |
| title_fullStr | Unraveling Genomic and Pathogenic Features of <i>Aeromonas ichthyocola</i> sp. nov., <i>Aeromonas mytilicola</i> sp. nov., and <i>Aeromonas mytilicola</i> subsp. <i>aquatica</i> subsp. nov. |
| title_full_unstemmed | Unraveling Genomic and Pathogenic Features of <i>Aeromonas ichthyocola</i> sp. nov., <i>Aeromonas mytilicola</i> sp. nov., and <i>Aeromonas mytilicola</i> subsp. <i>aquatica</i> subsp. nov. |
| title_short | Unraveling Genomic and Pathogenic Features of <i>Aeromonas ichthyocola</i> sp. nov., <i>Aeromonas mytilicola</i> sp. nov., and <i>Aeromonas mytilicola</i> subsp. <i>aquatica</i> subsp. nov. |
| title_sort | unraveling genomic and pathogenic features of i aeromonas ichthyocola i sp nov i aeromonas mytilicola i sp nov and i aeromonas mytilicola i subsp i aquatica i subsp nov |
| topic | <i>Aeromonas</i> <i>Galleria mellonella</i> genome analysis taxonomy |
| url | https://www.mdpi.com/2076-2615/15/7/948 |
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