Screening of β-caryophyllene-producing bacteria and optimization of the extraction process of β-caryophyllene
In order to screen the high-yield β-caryophyllene (BCP) strains, the aroma components in light-flavor (Qingxiangxing) Baijiu and Daqu was detected by headspace solid-phase microextraction (HS-SPME)-GC-MS. The β-caryophyllene-producing strains were preliminarily screened by traditional isolation tech...
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Editorial Department of China Brewing
2024-04-01
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| Series: | Zhongguo niangzao |
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| Online Access: | https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2024-43-4-91.pdf |
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| author | MOU Feiyan, HE Yimin, XIA Boyu, DONG Xiaoyuan, CHEN Hui, CHANG Xu, CHEN Maobin, FANG Shangling |
| author_facet | MOU Feiyan, HE Yimin, XIA Boyu, DONG Xiaoyuan, CHEN Hui, CHANG Xu, CHEN Maobin, FANG Shangling |
| author_sort | MOU Feiyan, HE Yimin, XIA Boyu, DONG Xiaoyuan, CHEN Hui, CHANG Xu, CHEN Maobin, FANG Shangling |
| collection | DOAJ |
| description | In order to screen the high-yield β-caryophyllene (BCP) strains, the aroma components in light-flavor (Qingxiangxing) Baijiu and Daqu was detected by headspace solid-phase microextraction (HS-SPME)-GC-MS. The β-caryophyllene-producing strains were preliminarily screened by traditional isolation techniques, Fuqu was inoculated with primary screening strains to re-screening by β-caryophyllene content determination, and identified by molecular biology technique. The extraction conditions of β-caryophyllene in Fuqu were optimized, and the screened strains were applied in small tank fermentation. The results showed that the β-caryophyllene content in the light-flavor original liquor was 0.02 g/L, and the relative content of β-caryophyllene in Daqu was 0.85%. Five strains of mold (SDA1-SDA5) were preliminarily screened, and the strain SDA3 with the highest relative content of β-caryophyllene (1.04%) was identified as Rhizopus delemar. The β-caryophyllene content was 37.98 mg/L when the extraction reagent was anhydrous ethanol and the extraction time was 2 h. Compared with the addition of Daqu alone, the β-caryophyllene content in liquor produced by adding strain SDA3 to enhance Fuqu for small tank fermentation was increased from 13.65 mg/L to 24.65 mg/L. |
| format | Article |
| id | doaj-art-466b3de001aa4a2e94d29dd0764f21ab |
| institution | DOAJ |
| issn | 0254-5071 |
| language | English |
| publishDate | 2024-04-01 |
| publisher | Editorial Department of China Brewing |
| record_format | Article |
| series | Zhongguo niangzao |
| spelling | doaj-art-466b3de001aa4a2e94d29dd0764f21ab2025-08-20T02:55:06ZengEditorial Department of China BrewingZhongguo niangzao0254-50712024-04-01434919710.11882/j.issn.0254-5071.2024.04.014Screening of β-caryophyllene-producing bacteria and optimization of the extraction process of β-caryophylleneMOU Feiyan, HE Yimin, XIA Boyu, DONG Xiaoyuan, CHEN Hui, CHANG Xu, CHEN Maobin, FANG Shangling01. Cooperative Innovation Center of Industrial Fermentation (Ministry of Education & Hubei Province), School of Food and Biological Engineering, Hubei University of Technology, Wuhan 430068, China; ;2. Huanghelou Distillery Co., Ltd., Wuhan 430000, China; ;3. Angel Yeast Co., Ltd., Yichang 443000, ChinaIn order to screen the high-yield β-caryophyllene (BCP) strains, the aroma components in light-flavor (Qingxiangxing) Baijiu and Daqu was detected by headspace solid-phase microextraction (HS-SPME)-GC-MS. The β-caryophyllene-producing strains were preliminarily screened by traditional isolation techniques, Fuqu was inoculated with primary screening strains to re-screening by β-caryophyllene content determination, and identified by molecular biology technique. The extraction conditions of β-caryophyllene in Fuqu were optimized, and the screened strains were applied in small tank fermentation. The results showed that the β-caryophyllene content in the light-flavor original liquor was 0.02 g/L, and the relative content of β-caryophyllene in Daqu was 0.85%. Five strains of mold (SDA1-SDA5) were preliminarily screened, and the strain SDA3 with the highest relative content of β-caryophyllene (1.04%) was identified as Rhizopus delemar. The β-caryophyllene content was 37.98 mg/L when the extraction reagent was anhydrous ethanol and the extraction time was 2 h. Compared with the addition of Daqu alone, the β-caryophyllene content in liquor produced by adding strain SDA3 to enhance Fuqu for small tank fermentation was increased from 13.65 mg/L to 24.65 mg/L.https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2024-43-4-91.pdfβ-caryophyllene|daqu|baijiu|mold|screening|identification|fortified fuqu |
| spellingShingle | MOU Feiyan, HE Yimin, XIA Boyu, DONG Xiaoyuan, CHEN Hui, CHANG Xu, CHEN Maobin, FANG Shangling Screening of β-caryophyllene-producing bacteria and optimization of the extraction process of β-caryophyllene Zhongguo niangzao β-caryophyllene|daqu|baijiu|mold|screening|identification|fortified fuqu |
| title | Screening of β-caryophyllene-producing bacteria and optimization of the extraction process of β-caryophyllene |
| title_full | Screening of β-caryophyllene-producing bacteria and optimization of the extraction process of β-caryophyllene |
| title_fullStr | Screening of β-caryophyllene-producing bacteria and optimization of the extraction process of β-caryophyllene |
| title_full_unstemmed | Screening of β-caryophyllene-producing bacteria and optimization of the extraction process of β-caryophyllene |
| title_short | Screening of β-caryophyllene-producing bacteria and optimization of the extraction process of β-caryophyllene |
| title_sort | screening of β caryophyllene producing bacteria and optimization of the extraction process of β caryophyllene |
| topic | β-caryophyllene|daqu|baijiu|mold|screening|identification|fortified fuqu |
| url | https://manu61.magtech.com.cn/zgnz/fileup/0254-5071/PDF/0254-5071-2024-43-4-91.pdf |
| work_keys_str_mv | AT moufeiyanheyiminxiaboyudongxiaoyuanchenhuichangxuchenmaobinfangshangling screeningofbcaryophylleneproducingbacteriaandoptimizationoftheextractionprocessofbcaryophyllene |