Integrative Analysis of Transcriptome and Metabolome Reveals the Regulatory Network Governing Aroma Formation in Grape
The aroma metabolites in grape berries have received attention in recent years, but a global analysis of gene-regulated metabolites is still lacking. In this study, three grape cultivars, “Kyoho”, “Adenauer Rose”, and “Mei Xiangbao”, were used to determine the differential accumulation of metabolite...
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MDPI AG
2024-10-01
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| Series: | Horticulturae |
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| author | Liping Huang Yue Zhu Min Wang Zhili Xun Xiaohe Ma Qifeng Zhao |
| author_facet | Liping Huang Yue Zhu Min Wang Zhili Xun Xiaohe Ma Qifeng Zhao |
| author_sort | Liping Huang |
| collection | DOAJ |
| description | The aroma metabolites in grape berries have received attention in recent years, but a global analysis of gene-regulated metabolites is still lacking. In this study, three grape cultivars, “Kyoho”, “Adenauer Rose”, and “Mei Xiangbao”, were used to determine the differential accumulation of metabolites and identify candidate genes related to grape berry aroma. A total of 27,228 genes were detected from the transcriptome, and 128 differentially accumulated metabolites (DAMs) were identified. Terpenoids and ester were the major substances in these three cultivars. KEGG enrichment showed that 12, 8, and 5 compounds were significantly enriched during the maturation process of these three grape cultivars, with most being terpenoids. A combined transcriptome and metabolome analysis found that the associated genes and metabolites were enriched in the following pathways: “Glycine, serine, and threonine metabolism”, “Cysteine and methionine metabolism”, “Tyrosine metabolism”, “Phenylalanine metabolism”, and “Phenylalanine, tyrosine, and tryptophan biosynthesis”. Seven structural genes (<i>VvOMR1</i>, <i>VvGLYK</i>, <i>VvLPD2</i>, <i>VvAK2</i>, <i>VvSHM7</i>, <i>VvASP3</i>, and <i>VvASP1</i>) and four transcription factors (<i>Vv</i>ERF053, <i>Vv</i>ERF4, <i>Vv</i>MYB46, and <i>Vv</i>MYB340) related to grape berry aroma accumulation were discovered. Our findings provide new insights into grape aroma formation and regulatory mechanism research, and the results will be beneficial for grape aroma breeding in the future. |
| format | Article |
| id | doaj-art-4665860dcd6044f59b8a6afb2b11d998 |
| institution | OA Journals |
| issn | 2311-7524 |
| language | English |
| publishDate | 2024-10-01 |
| publisher | MDPI AG |
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| series | Horticulturae |
| spelling | doaj-art-4665860dcd6044f59b8a6afb2b11d9982025-08-20T01:53:56ZengMDPI AGHorticulturae2311-75242024-10-011011115910.3390/horticulturae10111159Integrative Analysis of Transcriptome and Metabolome Reveals the Regulatory Network Governing Aroma Formation in GrapeLiping Huang0Yue Zhu1Min Wang2Zhili Xun3Xiaohe Ma4Qifeng Zhao5Pomology Institute, Shanxi Agricultural University, Jinzhong 030801, ChinaPomology Institute, Shanxi Agricultural University, Jinzhong 030801, ChinaPomology Institute, Shanxi Agricultural University, Jinzhong 030801, ChinaPomology Institute, Shanxi Agricultural University, Jinzhong 030801, ChinaPomology Institute, Shanxi Agricultural University, Jinzhong 030801, ChinaPomology Institute, Shanxi Agricultural University, Jinzhong 030801, ChinaThe aroma metabolites in grape berries have received attention in recent years, but a global analysis of gene-regulated metabolites is still lacking. In this study, three grape cultivars, “Kyoho”, “Adenauer Rose”, and “Mei Xiangbao”, were used to determine the differential accumulation of metabolites and identify candidate genes related to grape berry aroma. A total of 27,228 genes were detected from the transcriptome, and 128 differentially accumulated metabolites (DAMs) were identified. Terpenoids and ester were the major substances in these three cultivars. KEGG enrichment showed that 12, 8, and 5 compounds were significantly enriched during the maturation process of these three grape cultivars, with most being terpenoids. A combined transcriptome and metabolome analysis found that the associated genes and metabolites were enriched in the following pathways: “Glycine, serine, and threonine metabolism”, “Cysteine and methionine metabolism”, “Tyrosine metabolism”, “Phenylalanine metabolism”, and “Phenylalanine, tyrosine, and tryptophan biosynthesis”. Seven structural genes (<i>VvOMR1</i>, <i>VvGLYK</i>, <i>VvLPD2</i>, <i>VvAK2</i>, <i>VvSHM7</i>, <i>VvASP3</i>, and <i>VvASP1</i>) and four transcription factors (<i>Vv</i>ERF053, <i>Vv</i>ERF4, <i>Vv</i>MYB46, and <i>Vv</i>MYB340) related to grape berry aroma accumulation were discovered. Our findings provide new insights into grape aroma formation and regulatory mechanism research, and the results will be beneficial for grape aroma breeding in the future.https://www.mdpi.com/2311-7524/10/11/1159aromametabolometranscriptomecandidate genesgrape breedingregulatory mechanism |
| spellingShingle | Liping Huang Yue Zhu Min Wang Zhili Xun Xiaohe Ma Qifeng Zhao Integrative Analysis of Transcriptome and Metabolome Reveals the Regulatory Network Governing Aroma Formation in Grape Horticulturae aroma metabolome transcriptome candidate genes grape breeding regulatory mechanism |
| title | Integrative Analysis of Transcriptome and Metabolome Reveals the Regulatory Network Governing Aroma Formation in Grape |
| title_full | Integrative Analysis of Transcriptome and Metabolome Reveals the Regulatory Network Governing Aroma Formation in Grape |
| title_fullStr | Integrative Analysis of Transcriptome and Metabolome Reveals the Regulatory Network Governing Aroma Formation in Grape |
| title_full_unstemmed | Integrative Analysis of Transcriptome and Metabolome Reveals the Regulatory Network Governing Aroma Formation in Grape |
| title_short | Integrative Analysis of Transcriptome and Metabolome Reveals the Regulatory Network Governing Aroma Formation in Grape |
| title_sort | integrative analysis of transcriptome and metabolome reveals the regulatory network governing aroma formation in grape |
| topic | aroma metabolome transcriptome candidate genes grape breeding regulatory mechanism |
| url | https://www.mdpi.com/2311-7524/10/11/1159 |
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