The Impact of MSTN Gene Editing on Meat Quality and Metabolomics: A Comparative Study Among Three Breeds of MSTN-Edited and Non-Edited Cattle
Myostatin (MSTN) serves as a negative regulatory factor for muscle development. A reduction in MSTN gene expression can enhance muscle mass and increase meat production. However, whether it will impact meat quality traits remains one of the major concerns in the cattle breeding industry. To explore...
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2024-12-01
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| author | Shan Luo Yuanqing Liu Lige Bu Dezheng Wang Zhaoyu Wen Yuqing Yang Yanan Xu Di Wu Guangpeng Li Lei Yang |
| author_facet | Shan Luo Yuanqing Liu Lige Bu Dezheng Wang Zhaoyu Wen Yuqing Yang Yanan Xu Di Wu Guangpeng Li Lei Yang |
| author_sort | Shan Luo |
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| description | Myostatin (MSTN) serves as a negative regulatory factor for muscle development. A reduction in MSTN gene expression can enhance muscle mass and increase meat production. However, whether it will impact meat quality traits remains one of the major concerns in the cattle breeding industry. To explore the meat quality traits of MSTN gene-edited cattle, this study compared the meat quality traits of three MSTN gene-edited cattle breeds with those of non-gene-edited cattle, including Luxi, Angus, and Mongolian cattle, and further conducted an analysis in combination with metabolomics. We found that MSTN gene-edited cattle were superior to non-gene-edited cattle in terms of meat pH, shear force, and intramuscular fat content. However, no significant differences were observed in water-holding capacity, water content, and protein content of the meat. Metabolomics analysis revealed three upregulated differential metabolites common to MSTN gene-edited and non-gene-edited cattle across three breeds, namely lactoyl-valine, 3-phenylactic acid, and lactoyl-methionine. Some studies have indicated that these metabolites can improve the meat’s flavor. In this study, we compared the meat quality traits and metabolomics data between MSTN gene-edited and non-gene-edited cattle, and found that the meat quality of MSTN gene-edited cattle was superior to that of non-gene-edited cattle. |
| format | Article |
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| institution | Kabale University |
| issn | 2076-2615 |
| language | English |
| publishDate | 2024-12-01 |
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| spelling | doaj-art-4663c060808c44cd82f0c476ad6e10022025-01-10T13:13:53ZengMDPI AGAnimals2076-26152024-12-011514710.3390/ani15010047The Impact of MSTN Gene Editing on Meat Quality and Metabolomics: A Comparative Study Among Three Breeds of MSTN-Edited and Non-Edited CattleShan Luo0Yuanqing Liu1Lige Bu2Dezheng Wang3Zhaoyu Wen4Yuqing Yang5Yanan Xu6Di Wu7Guangpeng Li8Lei Yang9State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010021, ChinaState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010021, ChinaState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010021, ChinaState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010021, ChinaState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010021, ChinaState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010021, ChinaState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010021, ChinaState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010021, ChinaState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010021, ChinaState Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock, College of Life Science, Inner Mongolia University, Hohhot 010021, ChinaMyostatin (MSTN) serves as a negative regulatory factor for muscle development. A reduction in MSTN gene expression can enhance muscle mass and increase meat production. However, whether it will impact meat quality traits remains one of the major concerns in the cattle breeding industry. To explore the meat quality traits of MSTN gene-edited cattle, this study compared the meat quality traits of three MSTN gene-edited cattle breeds with those of non-gene-edited cattle, including Luxi, Angus, and Mongolian cattle, and further conducted an analysis in combination with metabolomics. We found that MSTN gene-edited cattle were superior to non-gene-edited cattle in terms of meat pH, shear force, and intramuscular fat content. However, no significant differences were observed in water-holding capacity, water content, and protein content of the meat. Metabolomics analysis revealed three upregulated differential metabolites common to MSTN gene-edited and non-gene-edited cattle across three breeds, namely lactoyl-valine, 3-phenylactic acid, and lactoyl-methionine. Some studies have indicated that these metabolites can improve the meat’s flavor. In this study, we compared the meat quality traits and metabolomics data between MSTN gene-edited and non-gene-edited cattle, and found that the meat quality of MSTN gene-edited cattle was superior to that of non-gene-edited cattle.https://www.mdpi.com/2076-2615/15/1/47MSTNgene editingmeat quality traitsmetabolomics |
| spellingShingle | Shan Luo Yuanqing Liu Lige Bu Dezheng Wang Zhaoyu Wen Yuqing Yang Yanan Xu Di Wu Guangpeng Li Lei Yang The Impact of MSTN Gene Editing on Meat Quality and Metabolomics: A Comparative Study Among Three Breeds of MSTN-Edited and Non-Edited Cattle Animals MSTN gene editing meat quality traits metabolomics |
| title | The Impact of MSTN Gene Editing on Meat Quality and Metabolomics: A Comparative Study Among Three Breeds of MSTN-Edited and Non-Edited Cattle |
| title_full | The Impact of MSTN Gene Editing on Meat Quality and Metabolomics: A Comparative Study Among Three Breeds of MSTN-Edited and Non-Edited Cattle |
| title_fullStr | The Impact of MSTN Gene Editing on Meat Quality and Metabolomics: A Comparative Study Among Three Breeds of MSTN-Edited and Non-Edited Cattle |
| title_full_unstemmed | The Impact of MSTN Gene Editing on Meat Quality and Metabolomics: A Comparative Study Among Three Breeds of MSTN-Edited and Non-Edited Cattle |
| title_short | The Impact of MSTN Gene Editing on Meat Quality and Metabolomics: A Comparative Study Among Three Breeds of MSTN-Edited and Non-Edited Cattle |
| title_sort | impact of mstn gene editing on meat quality and metabolomics a comparative study among three breeds of mstn edited and non edited cattle |
| topic | MSTN gene editing meat quality traits metabolomics |
| url | https://www.mdpi.com/2076-2615/15/1/47 |
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