Mutational analysis of antibiotic resistance genes in Helicobacter pylori from Ghanaian dyspepsia patients: Implications for treatment strategies
Background: Antibiotic resistance jeopardizes the effectiveness of conventional treatment regimens for Helicobacter pylori infections, and this remains a major global health concern. H. pylori genes mutations negatively affect actions of most first line antibiotics. This study aimed to perform mutat...
Saved in:
| Main Authors: | , , , , , , , , |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Elsevier
2025-06-01
|
| Series: | Aspects of Molecular Medicine |
| Subjects: | |
| Online Access: | http://www.sciencedirect.com/science/article/pii/S2949688825000164 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1850208035843080192 |
|---|---|
| author | Eric Gyamerah Ofori Foster Kyei Emmanuel Ayitey Tagoe Ansumana Sandy Bockarie Samuel Mawuli Adadey Osbourne Quaye Michael Buenor Adinortey Gordon Akanzuwine Awandare Cynthia Ayefoumi Adinortey |
| author_facet | Eric Gyamerah Ofori Foster Kyei Emmanuel Ayitey Tagoe Ansumana Sandy Bockarie Samuel Mawuli Adadey Osbourne Quaye Michael Buenor Adinortey Gordon Akanzuwine Awandare Cynthia Ayefoumi Adinortey |
| author_sort | Eric Gyamerah Ofori |
| collection | DOAJ |
| description | Background: Antibiotic resistance jeopardizes the effectiveness of conventional treatment regimens for Helicobacter pylori infections, and this remains a major global health concern. H. pylori genes mutations negatively affect actions of most first line antibiotics. This study aimed to perform mutational analysis on H. pylori antibiotic resistance genes in Ghanaian patients diagnosed with dyspepsia. Materials and methods: Antrum gastric biopsies were taken from 169 study participants, minced in Brain Heart Infusion broth and cultured. Sensitivity to antibiotics of H. pylori isolates was determined by disc diffusion. Extracted DNA were amplified and antibiotic resistance genes gyrA, pbp1, and rdxA sequenced. Resistance genes were analysed for base and point mutations using online databases and Ugene 45.0 software. Results: Using rapid urease test, H. pylori infection prevalence was estimated to be 61%. Phenotypically, no sensitivity was recorded for metronidazole, amoxicillin, clarithromycin, and amoxicillin-clavulanic acid against the tested isolates. Resistance to levofloxacin was found to be 40% while 20% was recorded for each of tetracycline and ciprofloxacin. Mutations identified included G242 C/A, T254I, and S417T for pbp1 gene in amoxicillin resistance; K2N, Q6H, Q50Stop, E75K, R90K, G98S, H99P, R131K, and A183V for rdxA gene; N87I/T, A97V, M191I, V199 M/A, H200Y, and G208E for gyrA gene in levofloxacin resistance. Conclusions: There is high H. pylori antibiotic resistance in the region with amoxicillin, metronidazole, amoxicillin-clavulanic acid and clarithromycin showing no sensitivity to tested isolates. Tetracycline and ciprofloxacin may be more appropriate therapeutic regimen options against H. pylori. Observed resistance could be due to mutations in rdxA, pbp1, and gyrA genes. |
| format | Article |
| id | doaj-art-465b1100a7eb47fcab726c832491c593 |
| institution | OA Journals |
| issn | 2949-6888 |
| language | English |
| publishDate | 2025-06-01 |
| publisher | Elsevier |
| record_format | Article |
| series | Aspects of Molecular Medicine |
| spelling | doaj-art-465b1100a7eb47fcab726c832491c5932025-08-20T02:10:20ZengElsevierAspects of Molecular Medicine2949-68882025-06-01510007810.1016/j.amolm.2025.100078Mutational analysis of antibiotic resistance genes in Helicobacter pylori from Ghanaian dyspepsia patients: Implications for treatment strategiesEric Gyamerah Ofori0Foster Kyei1Emmanuel Ayitey Tagoe2Ansumana Sandy Bockarie3Samuel Mawuli Adadey4Osbourne Quaye5Michael Buenor Adinortey6Gordon Akanzuwine Awandare7Cynthia Ayefoumi Adinortey8Department of Biology Education, Faculty of Science Education, University of Education, Winneba, GhanaDepartment of Molecular Biology and Biotechnology, School of Biological Sciences, College of Agriculture and Natural Sciences, University of Cape Coast, Cape Coast, GhanaDepartment of Medical Laboratory Science, School of Biomedical and Allied Health Sciences, College of Health and Allied Sciences, University of Ghana, Legon, GhanaDepartment of Internal Medicine, School of Medical Sciences, College of Health and Allied Sciences, University of Cape Coast, Cape Coast, GhanaDepartment of Biochemistry, Cell and Molecular Biology, School of Biological Sciences, College of Basic and Applied Sciences, University of Ghana, Legon, Ghana; West African Centre for Cell Biology and Infectious Pathogens, University of Ghana, Legon, Ghana; University of Cape Town: Rondebosch, Western Cape, Republic of South AfricaDepartment of Biochemistry, Cell and Molecular Biology, School of Biological Sciences, College of Basic and Applied Sciences, University of Ghana, Legon, Ghana; West African Centre for Cell Biology and Infectious Pathogens, University of Ghana, Legon, GhanaDepartment of Biochemistry, School of Biological Sciences, College of Agriculture and Natural Sciences, University of Cape Coast, Cape Coast, GhanaDepartment of Biochemistry, Cell and Molecular Biology, School of Biological Sciences, College of Basic and Applied Sciences, University of Ghana, Legon, Ghana; West African Centre for Cell Biology and Infectious Pathogens, University of Ghana, Legon, GhanaDepartment of Molecular Biology and Biotechnology, School of Biological Sciences, College of Agriculture and Natural Sciences, University of Cape Coast, Cape Coast, Ghana; Corresponding author. Department of Molecular Biology and Biotechnology, University of Cape Coast, Ghana.Background: Antibiotic resistance jeopardizes the effectiveness of conventional treatment regimens for Helicobacter pylori infections, and this remains a major global health concern. H. pylori genes mutations negatively affect actions of most first line antibiotics. This study aimed to perform mutational analysis on H. pylori antibiotic resistance genes in Ghanaian patients diagnosed with dyspepsia. Materials and methods: Antrum gastric biopsies were taken from 169 study participants, minced in Brain Heart Infusion broth and cultured. Sensitivity to antibiotics of H. pylori isolates was determined by disc diffusion. Extracted DNA were amplified and antibiotic resistance genes gyrA, pbp1, and rdxA sequenced. Resistance genes were analysed for base and point mutations using online databases and Ugene 45.0 software. Results: Using rapid urease test, H. pylori infection prevalence was estimated to be 61%. Phenotypically, no sensitivity was recorded for metronidazole, amoxicillin, clarithromycin, and amoxicillin-clavulanic acid against the tested isolates. Resistance to levofloxacin was found to be 40% while 20% was recorded for each of tetracycline and ciprofloxacin. Mutations identified included G242 C/A, T254I, and S417T for pbp1 gene in amoxicillin resistance; K2N, Q6H, Q50Stop, E75K, R90K, G98S, H99P, R131K, and A183V for rdxA gene; N87I/T, A97V, M191I, V199 M/A, H200Y, and G208E for gyrA gene in levofloxacin resistance. Conclusions: There is high H. pylori antibiotic resistance in the region with amoxicillin, metronidazole, amoxicillin-clavulanic acid and clarithromycin showing no sensitivity to tested isolates. Tetracycline and ciprofloxacin may be more appropriate therapeutic regimen options against H. pylori. Observed resistance could be due to mutations in rdxA, pbp1, and gyrA genes.http://www.sciencedirect.com/science/article/pii/S2949688825000164MutationAntibioticResistanceSusceptibleHelicobacter pyloriBiopsy |
| spellingShingle | Eric Gyamerah Ofori Foster Kyei Emmanuel Ayitey Tagoe Ansumana Sandy Bockarie Samuel Mawuli Adadey Osbourne Quaye Michael Buenor Adinortey Gordon Akanzuwine Awandare Cynthia Ayefoumi Adinortey Mutational analysis of antibiotic resistance genes in Helicobacter pylori from Ghanaian dyspepsia patients: Implications for treatment strategies Aspects of Molecular Medicine Mutation Antibiotic Resistance Susceptible Helicobacter pylori Biopsy |
| title | Mutational analysis of antibiotic resistance genes in Helicobacter pylori from Ghanaian dyspepsia patients: Implications for treatment strategies |
| title_full | Mutational analysis of antibiotic resistance genes in Helicobacter pylori from Ghanaian dyspepsia patients: Implications for treatment strategies |
| title_fullStr | Mutational analysis of antibiotic resistance genes in Helicobacter pylori from Ghanaian dyspepsia patients: Implications for treatment strategies |
| title_full_unstemmed | Mutational analysis of antibiotic resistance genes in Helicobacter pylori from Ghanaian dyspepsia patients: Implications for treatment strategies |
| title_short | Mutational analysis of antibiotic resistance genes in Helicobacter pylori from Ghanaian dyspepsia patients: Implications for treatment strategies |
| title_sort | mutational analysis of antibiotic resistance genes in helicobacter pylori from ghanaian dyspepsia patients implications for treatment strategies |
| topic | Mutation Antibiotic Resistance Susceptible Helicobacter pylori Biopsy |
| url | http://www.sciencedirect.com/science/article/pii/S2949688825000164 |
| work_keys_str_mv | AT ericgyamerahofori mutationalanalysisofantibioticresistancegenesinhelicobacterpylorifromghanaiandyspepsiapatientsimplicationsfortreatmentstrategies AT fosterkyei mutationalanalysisofantibioticresistancegenesinhelicobacterpylorifromghanaiandyspepsiapatientsimplicationsfortreatmentstrategies AT emmanuelayiteytagoe mutationalanalysisofantibioticresistancegenesinhelicobacterpylorifromghanaiandyspepsiapatientsimplicationsfortreatmentstrategies AT ansumanasandybockarie mutationalanalysisofantibioticresistancegenesinhelicobacterpylorifromghanaiandyspepsiapatientsimplicationsfortreatmentstrategies AT samuelmawuliadadey mutationalanalysisofantibioticresistancegenesinhelicobacterpylorifromghanaiandyspepsiapatientsimplicationsfortreatmentstrategies AT osbournequaye mutationalanalysisofantibioticresistancegenesinhelicobacterpylorifromghanaiandyspepsiapatientsimplicationsfortreatmentstrategies AT michaelbuenoradinortey mutationalanalysisofantibioticresistancegenesinhelicobacterpylorifromghanaiandyspepsiapatientsimplicationsfortreatmentstrategies AT gordonakanzuwineawandare mutationalanalysisofantibioticresistancegenesinhelicobacterpylorifromghanaiandyspepsiapatientsimplicationsfortreatmentstrategies AT cynthiaayefoumiadinortey mutationalanalysisofantibioticresistancegenesinhelicobacterpylorifromghanaiandyspepsiapatientsimplicationsfortreatmentstrategies |