Agro-industrial Residues: Low-cost Biomass for the Production of Bioactive Extracellular Polysaccharides by Lactobacillus plantarum in Solid State Fermentation
Agro-residues, such as apple peel, banana peel, orange peel, tapioca peel, and sweet potato peel powders, were screened for the biosynthesis of bioactive extracellular polysaccharide (EPS) in solid-state fermentation. Lactobacillus plantarum utilized sweet potato peel and improved EPS production com...
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North Carolina State University
2025-07-01
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| Online Access: | https://ojs.bioresources.com/index.php/BRJ/article/view/23982 |
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| author | Hissah Abdulrahman Alodaini Rajamony Rajam Sheeja Joseph Joselin Ashraf Atef Hatamleh Munirah Abdullah Al-Dosary Sundaramani Alci Rani Ponnuswamy Vijayaraghavan Selvaraj Arokiyaraj |
| author_facet | Hissah Abdulrahman Alodaini Rajamony Rajam Sheeja Joseph Joselin Ashraf Atef Hatamleh Munirah Abdullah Al-Dosary Sundaramani Alci Rani Ponnuswamy Vijayaraghavan Selvaraj Arokiyaraj |
| author_sort | Hissah Abdulrahman Alodaini |
| collection | DOAJ |
| description | Agro-residues, such as apple peel, banana peel, orange peel, tapioca peel, and sweet potato peel powders, were screened for the biosynthesis of bioactive extracellular polysaccharide (EPS) in solid-state fermentation. Lactobacillus plantarum utilized sweet potato peel and improved EPS production compared with other wastes, so it was used for optimization studies. Compared with the unoptimized medium, the response surface methodology improved EPS production twofold during solid-state fermentation. In the central composite design, the experimental model was significant (p<0.01), and the selected variables (moisture, sucrose and yeast extract) significantly influenced EPS production. The maximum yield was 59.1 mg/g with 64.5% moisture content, 5.74% sucrose, and 1.39% yeast extract. In addition, the extracted EPS exhibited strong antioxidant activity, and it scavenged ABTS, DPPH, and hydroxyl radicals in a concentration-dependent manner. The antibacterial activity of EPS was greatest against Staphylococcus aureus, and the corresponding zone of inhibition was18±1 mm, followed by Pseudomonas aeruginosa(14±1 mm).The results showed that sweet potato peels can be used as a cheap substrate for EPS production in solid-state fermentation. |
| format | Article |
| id | doaj-art-46576424d9764083bb0f095ffef24559 |
| institution | Kabale University |
| issn | 1930-2126 |
| language | English |
| publishDate | 2025-07-01 |
| publisher | North Carolina State University |
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| series | BioResources |
| spelling | doaj-art-46576424d9764083bb0f095ffef245592025-08-20T03:45:03ZengNorth Carolina State UniversityBioResources1930-21262025-07-01203735873772207Agro-industrial Residues: Low-cost Biomass for the Production of Bioactive Extracellular Polysaccharides by Lactobacillus plantarum in Solid State FermentationHissah Abdulrahman Alodaini0Rajamony Rajam Sheeja1Joseph Joselin2Ashraf Atef Hatamleh3Munirah Abdullah Al-Dosary4Sundaramani Alci Rani5Ponnuswamy Vijayaraghavan6Selvaraj Arokiyaraj7Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Botany, Women’s Christian College (Affiliated to Manonmanium Sundaranar University, Tirunelveli - 627 012Department of Botany, Women’s Christian College (Affiliated to Manonmanium Sundaranar University, Tirunelveli - 627 012), Nagercoil, Kanniyakumari District, Tamil Nadu, India – 629 001Department of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Botany and Microbiology, College of Science, King Saud University, P.O. Box 2455, Riyadh 11451, Saudi ArabiaDepartment of Botany, Nesamony Memorial Christian College (Affiliated to Manonmanium Sundaranar University, Tirunelveli - 627 012), Marthandam, Kanniyakumari District, Tamil Nadu, India – 629 165Bioprocess Engineering Division, Smykon Biotech, Nagercoil, Kanniyakumri District, Tamilnadu, India-629001Department of Food Science & Biotechnology, Sejong University, Seoul 05006, South KoreaAgro-residues, such as apple peel, banana peel, orange peel, tapioca peel, and sweet potato peel powders, were screened for the biosynthesis of bioactive extracellular polysaccharide (EPS) in solid-state fermentation. Lactobacillus plantarum utilized sweet potato peel and improved EPS production compared with other wastes, so it was used for optimization studies. Compared with the unoptimized medium, the response surface methodology improved EPS production twofold during solid-state fermentation. In the central composite design, the experimental model was significant (p<0.01), and the selected variables (moisture, sucrose and yeast extract) significantly influenced EPS production. The maximum yield was 59.1 mg/g with 64.5% moisture content, 5.74% sucrose, and 1.39% yeast extract. In addition, the extracted EPS exhibited strong antioxidant activity, and it scavenged ABTS, DPPH, and hydroxyl radicals in a concentration-dependent manner. The antibacterial activity of EPS was greatest against Staphylococcus aureus, and the corresponding zone of inhibition was18±1 mm, followed by Pseudomonas aeruginosa(14±1 mm).The results showed that sweet potato peels can be used as a cheap substrate for EPS production in solid-state fermentation.https://ojs.bioresources.com/index.php/BRJ/article/view/23982agro-wastessweet potatovalorizationsolid-state fermentationextracellular polysaccharide |
| spellingShingle | Hissah Abdulrahman Alodaini Rajamony Rajam Sheeja Joseph Joselin Ashraf Atef Hatamleh Munirah Abdullah Al-Dosary Sundaramani Alci Rani Ponnuswamy Vijayaraghavan Selvaraj Arokiyaraj Agro-industrial Residues: Low-cost Biomass for the Production of Bioactive Extracellular Polysaccharides by Lactobacillus plantarum in Solid State Fermentation BioResources agro-wastes sweet potato valorization solid-state fermentation extracellular polysaccharide |
| title | Agro-industrial Residues: Low-cost Biomass for the Production of Bioactive Extracellular Polysaccharides by Lactobacillus plantarum in Solid State Fermentation |
| title_full | Agro-industrial Residues: Low-cost Biomass for the Production of Bioactive Extracellular Polysaccharides by Lactobacillus plantarum in Solid State Fermentation |
| title_fullStr | Agro-industrial Residues: Low-cost Biomass for the Production of Bioactive Extracellular Polysaccharides by Lactobacillus plantarum in Solid State Fermentation |
| title_full_unstemmed | Agro-industrial Residues: Low-cost Biomass for the Production of Bioactive Extracellular Polysaccharides by Lactobacillus plantarum in Solid State Fermentation |
| title_short | Agro-industrial Residues: Low-cost Biomass for the Production of Bioactive Extracellular Polysaccharides by Lactobacillus plantarum in Solid State Fermentation |
| title_sort | agro industrial residues low cost biomass for the production of bioactive extracellular polysaccharides by lactobacillus plantarum in solid state fermentation |
| topic | agro-wastes sweet potato valorization solid-state fermentation extracellular polysaccharide |
| url | https://ojs.bioresources.com/index.php/BRJ/article/view/23982 |
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