The Influence of Mg2+ Ions on the In Vitro Efficacy of Chitosan-Titanium Dioxide Nanotubes (CTNTs) Scaffolds
Low mechanical strength and lack of osteoconductive cues are problems associated with chitosan-based scaffolds. This research aimed to fabricate reinforced chitosan-titanium dioxide (TiO2) nanotubes (CTNTs) scaffolds attributed to the enhanced biocompatibility and physical properties of TiO2 nanotub...
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| Format: | Article |
| Language: | English |
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Wiley
2019-01-01
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| Series: | Advances in Polymer Technology |
| Online Access: | http://dx.doi.org/10.1155/2019/9679627 |
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| author | Siew Shee Lim Shui Wei Kho Nehemiah Li Heng Ang Cheng Heng Pang Hwei-San Loh |
| author_facet | Siew Shee Lim Shui Wei Kho Nehemiah Li Heng Ang Cheng Heng Pang Hwei-San Loh |
| author_sort | Siew Shee Lim |
| collection | DOAJ |
| description | Low mechanical strength and lack of osteoconductive cues are problems associated with chitosan-based scaffolds. This research aimed to fabricate reinforced chitosan-titanium dioxide (TiO2) nanotubes (CTNTs) scaffolds attributed to the enhanced biocompatibility and physical properties of TiO2 nanotubes (TNTs). The incorporation of hydrothermally synthesized TNTs at weight percent of 16 into chitosan was achieved via direct blending and lyophilization. CTNTs scaffolds were further subjected to 24-h adsorption in MgCl2 solutions of 0.5 mM, 1 mM, 2.5 mM, and 5 mM at physiological pH. The adsorption affinity of CTNTs towards Mg2+ ions was high and mainly attributed to the macroporosity of scaffolds and nanocavities of TNTs. The maximum monolayer adsorption capacity of CTNTs for Mg2+ ions was 8.8 mg/g scaffolds. Its adsorption isotherm fitted well with Langmuir isotherm by showing R2 of 0.9995. Fluorescence-based staining, cell viability, and alkaline phosphatase assays indicated that the adsorbed Mg2+ ions onto CTNTs scaffolds aided in promoting higher proliferation and early differentiation of MG63 cells than scaffolds without Mg2+ ions in a concentration-dependent manner. Based on current results, CTNTs scaffolds with Mg2+ ions may be a potential biomaterial for bone regeneration. |
| format | Article |
| id | doaj-art-4649fdc006514f389b44e79b39458d5f |
| institution | Kabale University |
| issn | 0730-6679 1098-2329 |
| language | English |
| publishDate | 2019-01-01 |
| publisher | Wiley |
| record_format | Article |
| series | Advances in Polymer Technology |
| spelling | doaj-art-4649fdc006514f389b44e79b39458d5f2025-02-03T01:11:53ZengWileyAdvances in Polymer Technology0730-66791098-23292019-01-01201910.1155/2019/96796279679627The Influence of Mg2+ Ions on the In Vitro Efficacy of Chitosan-Titanium Dioxide Nanotubes (CTNTs) ScaffoldsSiew Shee Lim0Shui Wei Kho1Nehemiah Li Heng Ang2Cheng Heng Pang3Hwei-San Loh4Department of Chemical and Environmental Engineering, University of Nottingham Malaysia Campus, Jalan Broga 43500 Semenyih, Selangor, MalaysiaDepartment of Chemical and Environmental Engineering, University of Nottingham Malaysia Campus, Jalan Broga 43500 Semenyih, Selangor, MalaysiaDepartment of Chemical and Environmental Engineering, University of Nottingham Malaysia Campus, Jalan Broga 43500 Semenyih, Selangor, MalaysiaDepartment of Chemical and Environmental Engineering, University of Nottingham Ningbo China, 199, Taikang East Road, Ningbo 315100, ChinaSchool of Biosciences, University of Nottingham Malaysia Campus, Jalan Broga 43500 Semenyih, Selangor, MalaysiaLow mechanical strength and lack of osteoconductive cues are problems associated with chitosan-based scaffolds. This research aimed to fabricate reinforced chitosan-titanium dioxide (TiO2) nanotubes (CTNTs) scaffolds attributed to the enhanced biocompatibility and physical properties of TiO2 nanotubes (TNTs). The incorporation of hydrothermally synthesized TNTs at weight percent of 16 into chitosan was achieved via direct blending and lyophilization. CTNTs scaffolds were further subjected to 24-h adsorption in MgCl2 solutions of 0.5 mM, 1 mM, 2.5 mM, and 5 mM at physiological pH. The adsorption affinity of CTNTs towards Mg2+ ions was high and mainly attributed to the macroporosity of scaffolds and nanocavities of TNTs. The maximum monolayer adsorption capacity of CTNTs for Mg2+ ions was 8.8 mg/g scaffolds. Its adsorption isotherm fitted well with Langmuir isotherm by showing R2 of 0.9995. Fluorescence-based staining, cell viability, and alkaline phosphatase assays indicated that the adsorbed Mg2+ ions onto CTNTs scaffolds aided in promoting higher proliferation and early differentiation of MG63 cells than scaffolds without Mg2+ ions in a concentration-dependent manner. Based on current results, CTNTs scaffolds with Mg2+ ions may be a potential biomaterial for bone regeneration.http://dx.doi.org/10.1155/2019/9679627 |
| spellingShingle | Siew Shee Lim Shui Wei Kho Nehemiah Li Heng Ang Cheng Heng Pang Hwei-San Loh The Influence of Mg2+ Ions on the In Vitro Efficacy of Chitosan-Titanium Dioxide Nanotubes (CTNTs) Scaffolds Advances in Polymer Technology |
| title | The Influence of Mg2+ Ions on the In Vitro Efficacy of Chitosan-Titanium Dioxide Nanotubes (CTNTs) Scaffolds |
| title_full | The Influence of Mg2+ Ions on the In Vitro Efficacy of Chitosan-Titanium Dioxide Nanotubes (CTNTs) Scaffolds |
| title_fullStr | The Influence of Mg2+ Ions on the In Vitro Efficacy of Chitosan-Titanium Dioxide Nanotubes (CTNTs) Scaffolds |
| title_full_unstemmed | The Influence of Mg2+ Ions on the In Vitro Efficacy of Chitosan-Titanium Dioxide Nanotubes (CTNTs) Scaffolds |
| title_short | The Influence of Mg2+ Ions on the In Vitro Efficacy of Chitosan-Titanium Dioxide Nanotubes (CTNTs) Scaffolds |
| title_sort | influence of mg2 ions on the in vitro efficacy of chitosan titanium dioxide nanotubes ctnts scaffolds |
| url | http://dx.doi.org/10.1155/2019/9679627 |
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