Whole genome sequencing and phylogenetic analysis of dengue virus in Central Nepal from 2022 to 2023

Abstract Background In Nepal, dengue is an emerging disease of growing concern as outbreaks are increasing in both size and geographic reach and beginning to affect areas previously thought dengue-free. Dengue genomic surveillance has previously been limited within Nepal; however, with the increase...

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Main Authors: Margaret Chi, Nishan Katuwal, Aastha Shrestha, Surendra Kumar Madhup, Dipesh Tamrakar, Rajeev Shrestha
Format: Article
Language:English
Published: BMC 2025-03-01
Series:BMC Global and Public Health
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Online Access:https://doi.org/10.1186/s44263-025-00135-z
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author Margaret Chi
Nishan Katuwal
Aastha Shrestha
Surendra Kumar Madhup
Dipesh Tamrakar
Rajeev Shrestha
author_facet Margaret Chi
Nishan Katuwal
Aastha Shrestha
Surendra Kumar Madhup
Dipesh Tamrakar
Rajeev Shrestha
author_sort Margaret Chi
collection DOAJ
description Abstract Background In Nepal, dengue is an emerging disease of growing concern as outbreaks are increasing in both size and geographic reach and beginning to affect areas previously thought dengue-free. Dengue genomic surveillance has previously been limited within Nepal; however, with the increase in accessibility to sequencing technologies since the COVID-19 pandemic, it has recently become more feasible. Methods This hospital-based retrospective study utilized banked samples from the 2022 and 2023 dengue seasons from Dhulikhel Hospital/Kathmandu University Hospital in Central Nepal. Next-generation sequencing was performed to obtain whole genome sequences of dengue virus which were analyzed phylogenetically using a maximum likelihood GTR + G model. Mutations were evaluated across viral particle region using the GISAID DengueServer. Results We obtained 41 full-length sequences of DENV from 80 PCR-positive samples, including 24 sequences (58.5%) from 2022 and 17 sequences (41.5%) from 2023. We identified a shift in the majority serotype of our samples from DENV1 in 2022 to DENV3 in 2023, though 3 out of the 4 serotypes were identified in both years. Phylogenetic analysis revealed clusters within genotype III of DENV1 and genotype III of DENV3 closely related to strains from an outbreak of DENV in northern India in 2018–2019. DENV2 sequences fell into the cosmopolitan genotype IV-A1 and IV-B2 clades and were related to sequences from South and Southeast Asia and the USA, pointing to the global nature of dengue transmission. NS3 showed the highest frequency of mutation, whereas NS2B, NS4, NS5, and E were the most conserved. The most common mutations found were substitutions L17M and T20I in the 2 K peptide. A high number of mutations were observed in DENV3, followed by DENV2, with some mutations being unique to specific serotypes and others matching previously reported strains. Conclusions We identified possible clade shifts in the DENV1 and 2 populations and a rising prevalence of DENV3. Our study showed a high level of serotype diversity of DENV circulating in Central Nepal. Furthermore, our results indicate that DENV populations in Nepal are related to a geographically diverse set of sequences but are most strongly influenced by Indian strains of DENV.
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spelling doaj-art-4648df132cf044d48e59a0ff9f2a232e2025-08-20T03:05:50ZengBMCBMC Global and Public Health2731-913X2025-03-013111210.1186/s44263-025-00135-zWhole genome sequencing and phylogenetic analysis of dengue virus in Central Nepal from 2022 to 2023Margaret Chi0Nishan Katuwal1Aastha Shrestha2Surendra Kumar Madhup3Dipesh Tamrakar4Rajeev Shrestha5Center for Infectious Disease Research and Surveillance, Dhulikhel Hospital Kathmandu University HospitalCenter for Infectious Disease Research and Surveillance, Dhulikhel Hospital Kathmandu University HospitalCenter for Infectious Disease Research and Surveillance, Dhulikhel Hospital Kathmandu University HospitalDepartment of Microbiology, Dhulikhel Hospital Kathmandu University HospitalCenter for Infectious Disease Research and Surveillance, Dhulikhel Hospital Kathmandu University HospitalCenter for Infectious Disease Research and Surveillance, Dhulikhel Hospital Kathmandu University HospitalAbstract Background In Nepal, dengue is an emerging disease of growing concern as outbreaks are increasing in both size and geographic reach and beginning to affect areas previously thought dengue-free. Dengue genomic surveillance has previously been limited within Nepal; however, with the increase in accessibility to sequencing technologies since the COVID-19 pandemic, it has recently become more feasible. Methods This hospital-based retrospective study utilized banked samples from the 2022 and 2023 dengue seasons from Dhulikhel Hospital/Kathmandu University Hospital in Central Nepal. Next-generation sequencing was performed to obtain whole genome sequences of dengue virus which were analyzed phylogenetically using a maximum likelihood GTR + G model. Mutations were evaluated across viral particle region using the GISAID DengueServer. Results We obtained 41 full-length sequences of DENV from 80 PCR-positive samples, including 24 sequences (58.5%) from 2022 and 17 sequences (41.5%) from 2023. We identified a shift in the majority serotype of our samples from DENV1 in 2022 to DENV3 in 2023, though 3 out of the 4 serotypes were identified in both years. Phylogenetic analysis revealed clusters within genotype III of DENV1 and genotype III of DENV3 closely related to strains from an outbreak of DENV in northern India in 2018–2019. DENV2 sequences fell into the cosmopolitan genotype IV-A1 and IV-B2 clades and were related to sequences from South and Southeast Asia and the USA, pointing to the global nature of dengue transmission. NS3 showed the highest frequency of mutation, whereas NS2B, NS4, NS5, and E were the most conserved. The most common mutations found were substitutions L17M and T20I in the 2 K peptide. A high number of mutations were observed in DENV3, followed by DENV2, with some mutations being unique to specific serotypes and others matching previously reported strains. Conclusions We identified possible clade shifts in the DENV1 and 2 populations and a rising prevalence of DENV3. Our study showed a high level of serotype diversity of DENV circulating in Central Nepal. Furthermore, our results indicate that DENV populations in Nepal are related to a geographically diverse set of sequences but are most strongly influenced by Indian strains of DENV.https://doi.org/10.1186/s44263-025-00135-zDengueWhole genome sequencingPhylogeneticsGenomicsNeglected tropical diseases
spellingShingle Margaret Chi
Nishan Katuwal
Aastha Shrestha
Surendra Kumar Madhup
Dipesh Tamrakar
Rajeev Shrestha
Whole genome sequencing and phylogenetic analysis of dengue virus in Central Nepal from 2022 to 2023
BMC Global and Public Health
Dengue
Whole genome sequencing
Phylogenetics
Genomics
Neglected tropical diseases
title Whole genome sequencing and phylogenetic analysis of dengue virus in Central Nepal from 2022 to 2023
title_full Whole genome sequencing and phylogenetic analysis of dengue virus in Central Nepal from 2022 to 2023
title_fullStr Whole genome sequencing and phylogenetic analysis of dengue virus in Central Nepal from 2022 to 2023
title_full_unstemmed Whole genome sequencing and phylogenetic analysis of dengue virus in Central Nepal from 2022 to 2023
title_short Whole genome sequencing and phylogenetic analysis of dengue virus in Central Nepal from 2022 to 2023
title_sort whole genome sequencing and phylogenetic analysis of dengue virus in central nepal from 2022 to 2023
topic Dengue
Whole genome sequencing
Phylogenetics
Genomics
Neglected tropical diseases
url https://doi.org/10.1186/s44263-025-00135-z
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