Upregulated Acute Systemic Inflammation-Related Genes based on Endotoxin Exposure Provide “Survival Benefit” or Create “High Risk of Death” in Leukaemia and Colon Cancer
Objective: Although endotoxin exposure has been shown to trigger innate immune responses and promote cancer, it has also been shown to prevent cancer formation. In our study, survival analysis was performed to determine whether the upregulated genes triggered by endotoxins have hazardous effects on...
Saved in:
| Main Author: | |
|---|---|
| Format: | Article |
| Language: | English |
| Published: |
Istanbul University Press
2024-12-01
|
| Series: | European Journal of Biology |
| Subjects: | |
| Online Access: | https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/F931BE2904F14B43A98DA27F31EB92D8 |
| Tags: |
Add Tag
No Tags, Be the first to tag this record!
|
| _version_ | 1849723473049419776 |
|---|---|
| author | Gizem Ayna Duran |
| author_facet | Gizem Ayna Duran |
| author_sort | Gizem Ayna Duran |
| collection | DOAJ |
| description | Objective: Although endotoxin exposure has been shown to trigger innate immune responses and promote cancer, it has also been shown to prevent cancer formation. In our study, survival analysis was performed to determine whether the upregulated genes triggered by endotoxins have hazardous effects on cancers or provide a survival benefit. Materials and Methods: Gene intensity values of control and bacterial endotoxin-administered individuals were obtained from the Gene Expression Omnibus database. Using the R “Linear Models for Microarray Data” package, differentially expressed gene analyses were conducted to determine genes that differ between healthy and bacterial endotoxin-administered samples. “ShinyGo 0.80” web-based tool was used to determine the disease types indicated by these genes. The “Kaplan-Meier Plotter” web-based tool was used to conduct survival analysis. Results: Genes that create an innate immune response to bacterial endotoxin exposure and are upregulated differently than in individuals without exposure were identified. According to gene enrichment analyses, the two main types of cancer identified were leukaemia/lymoma and colon cancer. We detected that MLF1, STAT5B, and BCL3 genes led to poor survival; however, the ARHGAP26 gene was protective for acute myeloid leukaemia patients. In the case of colon cancer, SMAD7 and TLR2 genes were determined as leading to “high risk of death”. Conclusion: Once the systemic inflammation-related genes identified in our study are confirmed through laboratory experiments in samples taken from solid tissue in the case of colon cancer and at the level of genes obtained from blood samples in leukemias, genetically targeted treatments will also be possible. |
| format | Article |
| id | doaj-art-462914b7445e40668dc6c6f0b1cd79f7 |
| institution | DOAJ |
| issn | 2618-6144 |
| language | English |
| publishDate | 2024-12-01 |
| publisher | Istanbul University Press |
| record_format | Article |
| series | European Journal of Biology |
| spelling | doaj-art-462914b7445e40668dc6c6f0b1cd79f72025-08-20T03:11:02ZengIstanbul University PressEuropean Journal of Biology2618-61442024-12-0183215215910.26650/EurJBiol.2024.1459040123456Upregulated Acute Systemic Inflammation-Related Genes based on Endotoxin Exposure Provide “Survival Benefit” or Create “High Risk of Death” in Leukaemia and Colon CancerGizem Ayna Duran0https://orcid.org/0000-0002-2168-753Xİzmir Ekonomi Üniversitesi, Izmir, TurkiyeObjective: Although endotoxin exposure has been shown to trigger innate immune responses and promote cancer, it has also been shown to prevent cancer formation. In our study, survival analysis was performed to determine whether the upregulated genes triggered by endotoxins have hazardous effects on cancers or provide a survival benefit. Materials and Methods: Gene intensity values of control and bacterial endotoxin-administered individuals were obtained from the Gene Expression Omnibus database. Using the R “Linear Models for Microarray Data” package, differentially expressed gene analyses were conducted to determine genes that differ between healthy and bacterial endotoxin-administered samples. “ShinyGo 0.80” web-based tool was used to determine the disease types indicated by these genes. The “Kaplan-Meier Plotter” web-based tool was used to conduct survival analysis. Results: Genes that create an innate immune response to bacterial endotoxin exposure and are upregulated differently than in individuals without exposure were identified. According to gene enrichment analyses, the two main types of cancer identified were leukaemia/lymoma and colon cancer. We detected that MLF1, STAT5B, and BCL3 genes led to poor survival; however, the ARHGAP26 gene was protective for acute myeloid leukaemia patients. In the case of colon cancer, SMAD7 and TLR2 genes were determined as leading to “high risk of death”. Conclusion: Once the systemic inflammation-related genes identified in our study are confirmed through laboratory experiments in samples taken from solid tissue in the case of colon cancer and at the level of genes obtained from blood samples in leukemias, genetically targeted treatments will also be possible.https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/F931BE2904F14B43A98DA27F31EB92D8cancerendotoxinsystemic inflammationbioinformaticssurvival |
| spellingShingle | Gizem Ayna Duran Upregulated Acute Systemic Inflammation-Related Genes based on Endotoxin Exposure Provide “Survival Benefit” or Create “High Risk of Death” in Leukaemia and Colon Cancer European Journal of Biology cancer endotoxin systemic inflammation bioinformatics survival |
| title | Upregulated Acute Systemic Inflammation-Related Genes based on Endotoxin Exposure Provide “Survival Benefit” or Create “High Risk of Death” in Leukaemia and Colon Cancer |
| title_full | Upregulated Acute Systemic Inflammation-Related Genes based on Endotoxin Exposure Provide “Survival Benefit” or Create “High Risk of Death” in Leukaemia and Colon Cancer |
| title_fullStr | Upregulated Acute Systemic Inflammation-Related Genes based on Endotoxin Exposure Provide “Survival Benefit” or Create “High Risk of Death” in Leukaemia and Colon Cancer |
| title_full_unstemmed | Upregulated Acute Systemic Inflammation-Related Genes based on Endotoxin Exposure Provide “Survival Benefit” or Create “High Risk of Death” in Leukaemia and Colon Cancer |
| title_short | Upregulated Acute Systemic Inflammation-Related Genes based on Endotoxin Exposure Provide “Survival Benefit” or Create “High Risk of Death” in Leukaemia and Colon Cancer |
| title_sort | upregulated acute systemic inflammation related genes based on endotoxin exposure provide survival benefit or create high risk of death in leukaemia and colon cancer |
| topic | cancer endotoxin systemic inflammation bioinformatics survival |
| url | https://cdn.istanbul.edu.tr/file/JTA6CLJ8T5/F931BE2904F14B43A98DA27F31EB92D8 |
| work_keys_str_mv | AT gizemaynaduran upregulatedacutesystemicinflammationrelatedgenesbasedonendotoxinexposureprovidesurvivalbenefitorcreatehighriskofdeathinleukaemiaandcoloncancer |