Screening of a Soil Tyrosianse Producing Bacterial Strain HM24 and Comparison of its Tyrosinase Activity in Different Mediums Containing Natural Sources of L-tyrosine

Tyrosinases are essential enzymes with diverse applications, such as breaking down phenolic compounds and producing melanin. Actinobacteria are one of the most notable tyrosinase producers. This study investigated tyrosinase-producing actinobacterial strains and evaluated enzyme activity in differen...

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Main Authors: Seyed Muhammad Hamid Malekpour, Moj Khaleghi, Alireza Akhtarpour
Format: Article
Language:English
Published: University of Mazandaran 2024-09-01
Series:Journal of Genetic Resources
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Online Access:https://sc.journals.umz.ac.ir/article_5051_87d6b6977df9b0763bddf3d2e737a976.pdf
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author Seyed Muhammad Hamid Malekpour
Moj Khaleghi
Alireza Akhtarpour
author_facet Seyed Muhammad Hamid Malekpour
Moj Khaleghi
Alireza Akhtarpour
author_sort Seyed Muhammad Hamid Malekpour
collection DOAJ
description Tyrosinases are essential enzymes with diverse applications, such as breaking down phenolic compounds and producing melanin. Actinobacteria are one of the most notable tyrosinase producers. This study investigated tyrosinase-producing actinobacterial strains and evaluated enzyme activity in different sources of L-tyrosine. Twenty strains were isolated from the soil of Langar, Kerman, Iran, with characteristics of actinobacteria (gram-positive filamentous or rod-shaped bacteria and powdery consistency colonies that stick firmly to agar surface). All strains were cultured in a medium with L-tyrosine to assess tyrosinase production. The best tyrosinase-producing strain was selected based on dark-colored colony formation. Also, enzyme activity was evaluated in the four mediums with different L-tyrosine sources (Tyrosine, Glucose-Yeast-Peptone (GYP), Soybean, and Peanut). On the other, the pigment produced by actinobacteria was investigated for significant properties such as Sun Protection Factor (SPF) and antioxidant activity. The results showed that the HM24 strain is the best strain for producing tyrosinase. The results showed that the HM24 strain was the best tyrosinase-producing strain, and the most enzyme activity was in the peanut medium as well as in the soybean meal substrate. According to the 16S rRNA sequencing, the HM24 strain belonged to the genus Microbacterium with 99.97% identity. The pigment produced by HM24 demonstrated an SPF of 134.36 and a 69% DPPH reduction, which indicated strong antioxidant activity. These findings underscore the significant potential of actinobacterial strains in various industrial applications. Considering the production of tyrosinase enzyme by strain HM24, its ability to efficiently utilize natural L-tyrosine sources, and the biological properties of the resulting pigment, this strain holds significant potential for applications in various industrial processes.
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spelling doaj-art-4624652ac923426fb7fff3913c1377622025-01-27T06:11:46ZengUniversity of MazandaranJournal of Genetic Resources2423-42572588-25892024-09-0110212113010.22080/jgr.2024.27382.13965051Screening of a Soil Tyrosianse Producing Bacterial Strain HM24 and Comparison of its Tyrosinase Activity in Different Mediums Containing Natural Sources of L-tyrosineSeyed Muhammad Hamid Malekpour0Moj Khaleghi1Alireza Akhtarpour2Department of Biology, Faculty of Science, Shahid Bahonar University of Kerman, Kerman, IranDepartment of Biology, Faculty of Science, Shahid Bahonar University of Kerman, Kerman, IranDepartment of Biology, Faculty of Science, Shahid Bahonar University of Kerman, Kerman, IranTyrosinases are essential enzymes with diverse applications, such as breaking down phenolic compounds and producing melanin. Actinobacteria are one of the most notable tyrosinase producers. This study investigated tyrosinase-producing actinobacterial strains and evaluated enzyme activity in different sources of L-tyrosine. Twenty strains were isolated from the soil of Langar, Kerman, Iran, with characteristics of actinobacteria (gram-positive filamentous or rod-shaped bacteria and powdery consistency colonies that stick firmly to agar surface). All strains were cultured in a medium with L-tyrosine to assess tyrosinase production. The best tyrosinase-producing strain was selected based on dark-colored colony formation. Also, enzyme activity was evaluated in the four mediums with different L-tyrosine sources (Tyrosine, Glucose-Yeast-Peptone (GYP), Soybean, and Peanut). On the other, the pigment produced by actinobacteria was investigated for significant properties such as Sun Protection Factor (SPF) and antioxidant activity. The results showed that the HM24 strain is the best strain for producing tyrosinase. The results showed that the HM24 strain was the best tyrosinase-producing strain, and the most enzyme activity was in the peanut medium as well as in the soybean meal substrate. According to the 16S rRNA sequencing, the HM24 strain belonged to the genus Microbacterium with 99.97% identity. The pigment produced by HM24 demonstrated an SPF of 134.36 and a 69% DPPH reduction, which indicated strong antioxidant activity. These findings underscore the significant potential of actinobacterial strains in various industrial applications. Considering the production of tyrosinase enzyme by strain HM24, its ability to efficiently utilize natural L-tyrosine sources, and the biological properties of the resulting pigment, this strain holds significant potential for applications in various industrial processes.https://sc.journals.umz.ac.ir/article_5051_87d6b6977df9b0763bddf3d2e737a976.pdfactinobacteriaantioxidantl-tyrosinespftyrosinase
spellingShingle Seyed Muhammad Hamid Malekpour
Moj Khaleghi
Alireza Akhtarpour
Screening of a Soil Tyrosianse Producing Bacterial Strain HM24 and Comparison of its Tyrosinase Activity in Different Mediums Containing Natural Sources of L-tyrosine
Journal of Genetic Resources
actinobacteria
antioxidant
l-tyrosine
spf
tyrosinase
title Screening of a Soil Tyrosianse Producing Bacterial Strain HM24 and Comparison of its Tyrosinase Activity in Different Mediums Containing Natural Sources of L-tyrosine
title_full Screening of a Soil Tyrosianse Producing Bacterial Strain HM24 and Comparison of its Tyrosinase Activity in Different Mediums Containing Natural Sources of L-tyrosine
title_fullStr Screening of a Soil Tyrosianse Producing Bacterial Strain HM24 and Comparison of its Tyrosinase Activity in Different Mediums Containing Natural Sources of L-tyrosine
title_full_unstemmed Screening of a Soil Tyrosianse Producing Bacterial Strain HM24 and Comparison of its Tyrosinase Activity in Different Mediums Containing Natural Sources of L-tyrosine
title_short Screening of a Soil Tyrosianse Producing Bacterial Strain HM24 and Comparison of its Tyrosinase Activity in Different Mediums Containing Natural Sources of L-tyrosine
title_sort screening of a soil tyrosianse producing bacterial strain hm24 and comparison of its tyrosinase activity in different mediums containing natural sources of l tyrosine
topic actinobacteria
antioxidant
l-tyrosine
spf
tyrosinase
url https://sc.journals.umz.ac.ir/article_5051_87d6b6977df9b0763bddf3d2e737a976.pdf
work_keys_str_mv AT seyedmuhammadhamidmalekpour screeningofasoiltyrosianseproducingbacterialstrainhm24andcomparisonofitstyrosinaseactivityindifferentmediumscontainingnaturalsourcesofltyrosine
AT mojkhaleghi screeningofasoiltyrosianseproducingbacterialstrainhm24andcomparisonofitstyrosinaseactivityindifferentmediumscontainingnaturalsourcesofltyrosine
AT alirezaakhtarpour screeningofasoiltyrosianseproducingbacterialstrainhm24andcomparisonofitstyrosinaseactivityindifferentmediumscontainingnaturalsourcesofltyrosine